Characterization of Lactobacillus sake isolates from dry-cured sausages by restriction fragment length polymorphism analysis of the 16S rRNA gene

1998 ◽  
Vol 84 (4) ◽  
pp. 600-606 ◽  
Author(s):  
Y. Sanz ◽  
M. Hernandez ◽  
M.A. Ferrus ◽  
J. Hernandez
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Fragment Length Polymorphism ◽  
Rrna Gene ◽  
Polymorphism Analysis ◽  
Lactobacillus Sake ◽  
The 16S Rrna Gene ◽  
Restriction Fragment Length

scholarly journals Application of New Primer-Enzyme Combinations to Terminal Restriction Fragment Length Polymorphism Profiling of Bacterial Populations in Human Feces

2003 ◽  
Vol 69 (2) ◽  
pp. 1251-1262 ◽  
Author(s):  
Koji Nagashima ◽  
Takayoshi Hisada ◽  
Maremi Sato ◽  
Jun Mochizuki
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Rrna Gene ◽  
The 16S Rrna Gene ◽  
Restriction Fragment Length

ABSTRACT New primer-enzyme combinations for terminal restriction fragment length polymorphism (T-RFLP) targeting of the 16S rRNA gene were constructed by using the T-RFLP analysis program (designated TAP T-RFLP) located at the Ribosomal Database Project website, and their performance was examined empirically. By using the fluorescently labeled 516f primer (Escherichia coli positions 516 to 532) and 1510r primer (positions 1510 to 1492), the 16S rRNA gene was amplified from human fecal DNA. The resulting amplified product was digested with RsaI plus BfaI or with BslI. When the T-RFLP was carried out with fecal DNAs from eight individuals, eight predominant operational taxonomic units (OTUs) were detected with RsaI and BfaI digestion and 14 predominant OTUs were detected with BslI digestion. The distribution of the OTUs was consistent with the results of the computer simulations with TAP T-RFLP. The T-RFLP analyses of the fecal DNAs from individuals gave characteristic profiles, while the variability of the T-RFLP profiles between duplicate DNA preparations from the same samples were minimal. This new T-RFLP method made it easy to predict what kind of intestinal bacterial group corresponded to each OTU on the basis of the terminal restriction fragment length compared with the conventional T-RFLP and, moreover, made it possible to identify the bacterial species that an OTU represents by cloning and sequencing.

scholarly journals Rapid Identification of Campylobacter,Arcobacter, and Helicobacter Isolates by PCR-Restriction Fragment Length Polymorphism Analysis of the 16S rRNA Gene

1999 ◽  
Vol 37 (12) ◽  
pp. 4158-4160 ◽  
Author(s):  
Stephen M. Marshall ◽  
Pasquale L. Melito ◽  
David L. Woodward ◽  
Wendy M. Johnson ◽  
Frank G. Rodgers ◽  
...  
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Rrna Gene ◽  
The 16S Rrna Gene ◽  
Restriction Fragment Length

A rapid two-step identification scheme based on PCR-restriction fragment length polymorphism (PCR-RFLP) analysis of the 16S rRNA gene was developed in order to differentiate isolates belonging to theCampylobacter, Arcobacter, andHelicobacter genera. For 158 isolates (26 reference cultures and 132 clinical isolates), specific RFLP patterns were obtained and species were successfully identified by this assay.

Polymerase Chain Reaction–Restriction Fragment Length Polymorphism Analysis of a 16S rRNA Gene Fragment for Authentication of Four Clam Species

2002 ◽  
Vol 65 (4) ◽  
pp. 692-695 ◽  
Author(s):  
ALICIA FERNÁNDEZ ◽  
TERESA GARCÍA ◽  
ISABEL GONZÁLEZ ◽  
LUIS ASENSIO ◽  
MIGUEL ÁNGEL RODRÍGUEZ ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Restriction Fragment Length Polymorphism ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Fragment Length Polymorphism ◽  
Rrna Gene ◽  
Polymorphism Analysis ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Restriction Fragment Length

Specific identification of four clam species, Ruditapes decussatus (grooved carpet shell), Venerupis pullastra (pullet carpet shell), Ruditapes philippinarum (Japanese carpet shell), and Venerupis rhomboides (yellow carpet shell), was achieved by polymerase chain reaction–restriction fragment length polymorphism analysis of a fragment of the mitochondrial 16S rRNA gene. Amplification of DNA isolated from the foot muscle produced fragments of 511 bp for V. pullastra, 523 bp for R. decussatus, 545 bp for R. philippinarum, and 502 bp for V. rhomboides. The restriction profiles obtained by agarose gel electrophoresis when amplicons were digested with endonucleases BsmAI and BsrI allowed unequivocal identification of the four clam species. This approach would be less costly, simpler, and quicker than conventional sequencing of polymerase chain reaction products followed by detailed comparison of individual sequences, especially when large numbers of samples need to be analyzed.

Sign in / Sign up

Export Citation Format

Share Document