scholarly journals Achieving sub-diffraction spatial resolution using combined Fourier transform spectroscopy and nonlinear optical microscopy

2021 ◽  
Author(s):  
Megan A Steves ◽  
Kenneth Knappenberger, Jr.
Keyword(s):  
Fourier Transform ◽  
Optical Microscopy ◽  
Spatial Resolution ◽  
Nonlinear Optical ◽  
Fourier Transform Spectroscopy ◽  
Nonlinear Optical Microscopy

Aggregation-Induced Nonlinear Optical Effects of AIEgen Nanocrystals for Ultra-Deep in Vivo Bio-Imaging

2019 ◽  
Author(s):  
Zheng Zheng ◽  
Dongyu Li ◽  
zhiyang liu ◽  
Hui-Qing Peng ◽  
Herman H.-Y. Sung ◽  
...  
Keyword(s):  
Optical Microscopy ◽  
Spatial Resolution ◽  
Nonlinear Optical ◽  
Near Infrared ◽  
Simple Method ◽  
Nonlinear Optical Microscopy ◽  
Optical Effects ◽  
Nonlinear Optical Effects ◽  
Photon Fluorescence

<p><a></a>Nonlinear optical microscopy has become a powerful tool in bioimaging research due to its unique capabilities of deep optical sectioning, high spatial resolution imaging and three-dimensional reconstruction of biological specimens. Developing organic fluorescent probes with strong nonlinear optical effects, in particular third-harmonic generation (THG), is promising for exploiting nonlinear microscopic imaging for biomedical applications. Herein, we succesfully demonstrate a simple method for preparing organic nanocrystals based on an aggregation-induced emission (AIE) luminogen (DCCN) with bright near-infrared emission. Under femtosecond laser excitation, the high-order nonlinear optical effects of DCCN were studied in three distinct systems, including monomolecules in solution, amorphous nanopaticles, and crystaline nanopaticles. Results revealed aggregation-induced nonlinear optical (AINLO) effects, including two-photon fluorescence (2PF), three-photon fluorescence (3PF) and THG, of DCCN in nanopaticles, especially for the crystaline nanopaticles. Taking advantage of the strong 2PF and THG properties, the nanocrystals of DCCN have been successfully applied for 2PF microscopy at 1040 nm NIR-II excitation and THG microscopy at 1560 nm NIR-II excitation, respectively, to reconstruct the 3D vasculature of the mouse cerebral vasculature. Impressively, the THG microscopy could provide much higher spatial resolution and brightness than the 2PF microscopy and could visualize small vessels with diameters of ~2.7 μm at deepest depth of 800 μm in mouse brain, which is among the largest penetration depth and best spatial resolution of in vivo THG vasculature imaging. Thus, this is expected to inspire new insights into development of advanced AIE materials with multiple nonlinearity, in particular THG, for multimodal nonlinear optical microscopy.<br></p>

Aggregation-Induced Nonlinear Optical Effects of AIEgen Nanocrystals for Ultra-Deep in Vivo Bio-Imaging

2019 ◽  
Author(s):  
Zheng Zheng ◽  
Dongyu Li ◽  
zhiyang liu ◽  
Hui-Qing Peng ◽  
Herman H.-Y. Sung ◽  
...  
Keyword(s):  
Optical Microscopy ◽  
Spatial Resolution ◽  
Nonlinear Optical ◽  
Near Infrared ◽  
Simple Method ◽  
Nonlinear Optical Microscopy ◽  
Optical Effects ◽  
Nonlinear Optical Effects ◽  
Photon Fluorescence

<p><a></a>Nonlinear optical microscopy has become a powerful tool in bioimaging research due to its unique capabilities of deep optical sectioning, high spatial resolution imaging and three-dimensional reconstruction of biological specimens. Developing organic fluorescent probes with strong nonlinear optical effects, in particular third-harmonic generation (THG), is promising for exploiting nonlinear microscopic imaging for biomedical applications. Herein, we succesfully demonstrate a simple method for preparing organic nanocrystals based on an aggregation-induced emission (AIE) luminogen (DCCN) with bright near-infrared emission. Under femtosecond laser excitation, the high-order nonlinear optical effects of DCCN were studied in three distinct systems, including monomolecules in solution, amorphous nanopaticles, and crystaline nanopaticles. Results revealed aggregation-induced nonlinear optical (AINLO) effects, including two-photon fluorescence (2PF), three-photon fluorescence (3PF) and THG, of DCCN in nanopaticles, especially for the crystaline nanopaticles. Taking advantage of the strong 2PF and THG properties, the nanocrystals of DCCN have been successfully applied for 2PF microscopy at 1040 nm NIR-II excitation and THG microscopy at 1560 nm NIR-II excitation, respectively, to reconstruct the 3D vasculature of the mouse cerebral vasculature. Impressively, the THG microscopy could provide much higher spatial resolution and brightness than the 2PF microscopy and could visualize small vessels with diameters of ~2.7 μm at deepest depth of 800 μm in mouse brain, which is among the largest penetration depth and best spatial resolution of in vivo THG vasculature imaging. Thus, this is expected to inspire new insights into development of advanced AIE materials with multiple nonlinearity, in particular THG, for multimodal nonlinear optical microscopy.<br></p>

Nonlinear optical microscopy for artworks physics

2021 ◽  
Author(s):  
Alice Dal Fovo ◽  
Marta Castillejo ◽  
Raffaella Fontana
Keyword(s):  
Optical Microscopy ◽  
Nonlinear Optical ◽  
Nonlinear Optical Microscopy

scholarly journals Implementation of spatial overlap modulation nonlinear optical microscopy using an electro-optic deflector

2013 ◽  
Vol 4 (10) ◽  
pp. 1937 ◽  
Author(s):  
Keisuke Isobe ◽  
Hiroyuki Kawano ◽  
Akiko Kumagai ◽  
Atsushi Miyawaki ◽  
Katsumi Midorikawa
Keyword(s):  
Optical Microscopy ◽  
Nonlinear Optical ◽  
Nonlinear Optical Microscopy ◽  
Spatial Overlap ◽  
Electro Optic

scholarly journals Nonlinear optical microscopy and computational analysis of intrinsic signatures in breast cancer

2009 ◽  
Author(s):  
C.T. Rueden ◽  
M.W. Conklin ◽  
P.P. Provenzano ◽  
P.J. Keely ◽  
K.W. Eliceiri
Keyword(s):  
Breast Cancer ◽  
Optical Microscopy ◽  
Nonlinear Optical ◽  
Computational Analysis ◽  
Nonlinear Optical Microscopy ◽  
Intrinsic Signatures

Label-Free Characterization of Collagen Crosslinking in Bone-Engineered Materials Using Nonlinear Optical Microscopy

2021 ◽  
pp. 1-11
Author(s):  
Chao-Wei Hung ◽  
Nirmal Mazumder ◽  
Dan-Jae Lin ◽  
Wei-Liang Chen ◽  
Shih-Ting Lin ◽  
...  
Keyword(s):  
Optical Microscopy ◽  
Nonlinear Optical ◽  
Label Free ◽  
Nonlinear Optical Microscopy ◽  
Collagen Crosslinking ◽  
Engineered Materials

Abstract

Assessment of colloid response by nonlinear optical microscopy after preoperative radiochemotherapy for rectal carcinoma

2014 ◽  
Vol 20 (5) ◽  
pp. 051009 ◽  
Author(s):  
Lianhuang Li ◽  
Zhifen Chen ◽  
Xingfu Wang ◽  
Shuangmu Zhuo ◽  
Hongsheng Li ◽  
...  
Keyword(s):  
Optical Microscopy ◽  
Rectal Carcinoma ◽  
Nonlinear Optical ◽  
Preoperative Radiochemotherapy ◽  
Nonlinear Optical Microscopy

scholarly journals In situ 3D characterization of historical coatings and wood using multimodal nonlinear optical microscopy

2012 ◽  
Vol 20 (22) ◽  
pp. 24623 ◽  
Author(s):  
Gaël Latour ◽  
Jean-Philippe Echard ◽  
Marie Didier ◽  
Marie-Claire Schanne-Klein
Keyword(s):  
Optical Microscopy ◽  
Nonlinear Optical ◽  
Nonlinear Optical Microscopy
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