scholarly journals Regulatory region in choline acetyltransferase gene directs developmental and tissue-specific expression in transgenic mice.

1995 ◽  
Vol 92 (9) ◽  
pp. 4046-4050 ◽  
Author(s):  
P. Lonnerberg ◽  
U. Lendahl ◽  
H. Funakoshi ◽  
L. Arhlund-Richter ◽  
H. Persson ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Choline Acetyltransferase ◽  
Regulatory Region ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression

scholarly journals An upstream regulatory region mediates high-level, tissue-specific expression of the human alpha 1(I) collagen gene in transgenic mice.

1991 ◽  
Vol 11 (4) ◽  
pp. 2066-2074 ◽  
Author(s):  
J L Slack ◽  
D J Liska ◽  
P Bornstein
Keyword(s):  
Transgenic Mice ◽  
Regulatory Region ◽  
Collagen Gene ◽  
Flanking Sequence ◽  
Specific Expression ◽  
Tissue Specific ◽  
Endogenous Gene ◽  
Tissue Specific Expression ◽  
First Intron ◽  
High Level

Studies in vitro have not adequately resolved the role of intronic and upstream elements in regulating expression of the alpha 1(I) collagen gene. To address this issue, we generated 12 separate lines of transgenic mice with alpha 1(I) collagen-human growth hormone (hGH) constructs containing different amounts of 5'-flanking sequence, with or without most of the first intron. Transgenes driven by 2.3 kb of alpha 1(I) 5'-flanking sequence, whether or not they contained the first intron, were expressed at a high level and in a tissue-specific manner in seven out of seven independent lines of transgenic mice. In most tissues, the transgene was expressed at levels approaching that of the endogenous alpha 1(I) gene and was regulated identically with the endogenous gene as animals aged. However, in lung, expression of the transgene was anomalously high, and in muscle, expression was lower than that of the endogenous gene, suggesting that in these tissues other regions of the gene may participate in directing appropriate expression. Five lines of mice were generated containing transgenes driven by 0.44 kb of alpha 1(I) 5'-flanking sequence (with or without the first intron), and expression was detected in four out of five of these lines. The level of expression of the 0.44-kb constructs in the major collagen-producing tissues was 15- to 500-fold lower than that observed with the longer 2.3-kb promoter. While transgenes containing the 0.44-kb promoter and the first intron retained a modest degree of tissue-specific expression, those without the first intron lacked tissue specificity and were poorly expressed in all tissues except lung.(ABSTRACT TRUNCATED AT 250 WORDS)

An upstream regulatory region mediates high-level, tissue-specific expression of the human alpha 1(I) collagen gene in transgenic mice

1991 ◽  
Vol 11 (4) ◽  
pp. 2066-2074
Author(s):  
J L Slack ◽  
D J Liska ◽  
P Bornstein
Keyword(s):  
Transgenic Mice ◽  
Regulatory Region ◽  
Collagen Gene ◽  
Flanking Sequence ◽  
Specific Expression ◽  
Tissue Specific ◽  
Endogenous Gene ◽  
Tissue Specific Expression ◽  
First Intron ◽  
High Level

Studies in vitro have not adequately resolved the role of intronic and upstream elements in regulating expression of the alpha 1(I) collagen gene. To address this issue, we generated 12 separate lines of transgenic mice with alpha 1(I) collagen-human growth hormone (hGH) constructs containing different amounts of 5'-flanking sequence, with or without most of the first intron. Transgenes driven by 2.3 kb of alpha 1(I) 5'-flanking sequence, whether or not they contained the first intron, were expressed at a high level and in a tissue-specific manner in seven out of seven independent lines of transgenic mice. In most tissues, the transgene was expressed at levels approaching that of the endogenous alpha 1(I) gene and was regulated identically with the endogenous gene as animals aged. However, in lung, expression of the transgene was anomalously high, and in muscle, expression was lower than that of the endogenous gene, suggesting that in these tissues other regions of the gene may participate in directing appropriate expression. Five lines of mice were generated containing transgenes driven by 0.44 kb of alpha 1(I) 5'-flanking sequence (with or without the first intron), and expression was detected in four out of five of these lines. The level of expression of the 0.44-kb constructs in the major collagen-producing tissues was 15- to 500-fold lower than that observed with the longer 2.3-kb promoter. While transgenes containing the 0.44-kb promoter and the first intron retained a modest degree of tissue-specific expression, those without the first intron lacked tissue specificity and were poorly expressed in all tissues except lung.(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals Tissue-specific expression in the salivary glands of transgenic mice

1992 ◽  
Vol 20 (9) ◽  
pp. 2249-2255 ◽  
Author(s):  
Thomas R. Mikkelsen ◽  
Jakob Brandt ◽  
H.Jakob Larsen ◽  
Birte B. Larsen ◽  
Knud Poulsen ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Salivary Glands ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression

Tissue-specific expression of the rat pancreatic elastase I gene in transgenic mice

Cell ◽  
1984 ◽  
Vol 38 (3) ◽  
pp. 639-646 ◽  
Author(s):  
Galvin H. Swift ◽  
Robert E. Hammer ◽  
Raymond J. MacDonald ◽  
Ralph L. Brinster
Keyword(s):  
Transgenic Mice ◽  
Specific Expression ◽  
Tissue Specific ◽  
Pancreatic Elastase ◽  
Tissue Specific Expression ◽  
I Gene

Rat and Mouse Proopio-melanocortin Gene Sequences Target Tissue-Specific Expression to the Pituitary Gland but not to the Hypothalamus of Transgenic Mice

1993 ◽  
Vol 58 (4) ◽  
pp. 373-380 ◽  
Author(s):  
Marcelo Rubinstein ◽  
Marty Mortrud ◽  
Bin Liu ◽  
Malcolm J. Low
Keyword(s):  
Transgenic Mice ◽  
Pituitary Gland ◽  
Target Tissue ◽  
Gene Sequences ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression

scholarly journals Tissue-specific expression of a FMR1/β-galactosidase fusion gene in transgenic mice

1995 ◽  
Vol 4 (3) ◽  
pp. 359-366 ◽  
Author(s):  
Martin Hergersberg ◽  
Koichi Matsuo ◽  
Max Gassmann ◽  
Walter Schaffner ◽  
Bernhard Lüscher ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Fusion Gene ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression

scholarly journals Efficient tissue-specific expression of bovine alpha-lactalbumin in transgenic mice

1989 ◽  
Vol 186 (1-2) ◽  
pp. 43-48 ◽  
Author(s):  
Jean-Luc VILOTTE ◽  
Solange SOULIER ◽  
Marie-George STINNAKRE ◽  
Micheline MASSOUD ◽  
Jean-Claude MERCIER
Keyword(s):  
Transgenic Mice ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression ◽  
Alpha Lactalbumin

scholarly journals Tissue-specific expression of the rat β-casein gene in transgenic mice

1988 ◽  
Vol 16 (3) ◽  
pp. 1027-1041 ◽  
Author(s):  
Kuo-Fen Lee ◽  
Francesco J. DeMayo ◽  
Suzanne H. Atiee ◽  
Jeffrey M. Rosen
Keyword(s):  
Transgenic Mice ◽  
Specific Expression ◽  
Tissue Specific ◽  
Casein Gene ◽  
Tissue Specific Expression

scholarly journals A 5'-upstream region of a bovine keratin 6 gene confers tissue-specific expression and hyperproliferation-related induction in transgenic mice.

1995 ◽  
Vol 92 (11) ◽  
pp. 4783-4787 ◽  
Author(s):  
A. Ramirez ◽  
M. Vidal ◽  
A. Bravo ◽  
F. Larcher ◽  
J. L. Jorcano
Keyword(s):  
Transgenic Mice ◽  
Upstream Region ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression ◽  
Keratin 6

Differential expression of the closely linked KISS1, REN, and FLJ10761 genes in transgenic mice

2004 ◽  
Vol 17 (1) ◽  
pp. 4-10 ◽  
Author(s):  
Ravi Nistala ◽  
Xiaoji Zhang ◽  
Curt D. Sigmund
Keyword(s):  
Transgenic Mice ◽  
Human Chromosome ◽  
Expression Profiles ◽  
Expression Patterns ◽  
Artificial Chromosome ◽  
Chromosome 1 ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression ◽  
Human Chromosome 1

We previously reported the development and characterization of transgenic mice containing a large 160-kb P1 artificial chromosome (PAC) encompassing the renin (REN) locus from human chromosome 1. Here we demonstrate that PAC160 not only encodes REN, but also complete copies of the next upstream (KISS1) and downstream ( FLJ10761 ) gene along human chromosome 1. Incomplete copies of the second upstream (PEPP3) and downstream (SOX13) genes are also present. The gene order PEPP3-KISS1-REN-FLJ10761-SOX13 is conserved in mice containing either one or two copies of the REN locus. Despite the close localization of KISS1, REN, and FLJ10761 , they each exhibit distinct, yet overlapping tissue-specific expression profiles in humans. The tissue-specific expression patterns of REN and FLJ10761 were retained in transgenic mice containing PAC160. Expression of REN and FLJ10761 were also proportional to copy number. Expression of KISS1 in PAC160 mice showed both similarities and differences to humans. These data suggest that expression of gene blocks encoded on large genomic clones are retained when the clones are used to generate transgenic mice. Genomic elements which act to insulate genes from their neighbors are also apparently retained.

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