Phosphorylation of Sae2 Mediates Forkhead-associated (FHA) Domain-specific Interaction and Regulates Its DNA Repair Function

The RAD1 and RAD10 genes of Saccharomyces cerevisiae are two of at least seven genes which are known to be required for damage-specific recognition and/or damage-specific incision of DNA during nucleotide excision repair. RAD1 and RAD10 are also involved in a specialized mitotic recombination pathway. We have previously reported the purification of the RAD10 protein to homogeneity (L. Bardwell, H. Burtscher, W. A. Weiss, C. M. Nicolet, and E. C. Friedberg, Biochemistry 29:3119-3126, 1990). In the present studies we show that the RAD1 protein, produced by in vitro transcription and translation of the cloned gene, specifically coimmunoprecipitates with the RAD10 protein translated in vitro or purified from yeast. Conversely, in vitro-translated RAD10 protein specifically coimmunoprecipitates with the RAD1 protein. The sites of this stable and specific interaction have been mapped to the C-terminal regions of both polypeptides. This portion of RAD10 protein is evolutionarily conserved. These results are the first biochemical evidence of a specific association between any eukaryotic proteins genetically identified as belonging to a recombination or DNA repair pathway and suggest that the RAD1 and RAD10 proteins act at the same or consecutive biochemical steps in both nucleotide excision repair and mitotic recombination.

Download Full-text

Characterization of the specific interaction between archaeal FHA domain-containing protein and the promoter of a flagellar-like gene-cluster and its regulation by phosphorylation

Biochemical and Biophysical Research Communications ◽

10.1016/j.bbrc.2011.03.011 ◽

2011 ◽

Vol 407 (1) ◽

pp. 242-247 ◽

Cited By ~ 17

Author(s):

Xin Duan ◽

Zheng-Guo He

Keyword(s):

Gene Cluster ◽

Specific Interaction ◽

Fha Domain

Download Full-text

Stable and specific association between the yeast recombination and DNA repair proteins RAD1 and RAD10 in vitro.

Molecular and Cellular Biology ◽

10.1128/mcb.12.7.3041 ◽

1992 ◽

Vol 12 (7) ◽

pp. 3041-3049 ◽

Cited By ~ 47

Author(s):

L Bardwell ◽

A J Cooper ◽

E C Friedberg

Keyword(s):

Dna Repair ◽

Nucleotide Excision Repair ◽

Excision Repair ◽

Specific Interaction ◽

Mitotic Recombination ◽

Nucleotide Excision ◽

Specific Association ◽

Recombination Pathway ◽

Cloned Gene

The RAD1 and RAD10 genes of Saccharomyces cerevisiae are two of at least seven genes which are known to be required for damage-specific recognition and/or damage-specific incision of DNA during nucleotide excision repair. RAD1 and RAD10 are also involved in a specialized mitotic recombination pathway. We have previously reported the purification of the RAD10 protein to homogeneity (L. Bardwell, H. Burtscher, W. A. Weiss, C. M. Nicolet, and E. C. Friedberg, Biochemistry 29:3119-3126, 1990). In the present studies we show that the RAD1 protein, produced by in vitro transcription and translation of the cloned gene, specifically coimmunoprecipitates with the RAD10 protein translated in vitro or purified from yeast. Conversely, in vitro-translated RAD10 protein specifically coimmunoprecipitates with the RAD1 protein. The sites of this stable and specific interaction have been mapped to the C-terminal regions of both polypeptides. This portion of RAD10 protein is evolutionarily conserved. These results are the first biochemical evidence of a specific association between any eukaryotic proteins genetically identified as belonging to a recombination or DNA repair pathway and suggest that the RAD1 and RAD10 proteins act at the same or consecutive biochemical steps in both nucleotide excision repair and mitotic recombination.

Download Full-text

Domain-Specific Design of 3D Interaction Techniques: An Approach for Designing Useful Virtual Environment Applications

Presence Teleoperators & Virtual Environments ◽

10.1162/pres.18.5.370 ◽

2009 ◽

Vol 18 (5) ◽

pp. 370-386 ◽

Cited By ~ 8

Author(s):

Jian Chen ◽

Doug A Bowman

Keyword(s):

Virtual Environment ◽

Interaction Design ◽

Domain Knowledge ◽

Structural Engineering ◽

Specific Interaction ◽

Engineering Application ◽

Interaction Techniques ◽

3D Interaction ◽

Specific Design ◽

Domain Specific

Few production virtual environment (VE) applications involve complex three-dimensional (3D) interaction. Our long-term collaboration with architects and engineers in designing 3D user interfaces (3D UIs) has revealed some of the causes: existing interaction tasks and/or techniques are either too generic when isolated from the application context, or too specific to be reusable. We propose a new design approach called domain-specific design (DSD) that sits between the generic and specific design approaches, with an emphasis on using domain knowledge in 3D interaction techniques. We also describe an interaction design framework encompassing generic, domain-specific, and application-specific interaction tasks and techniques. This framework can be used by designers to think of ways to produce domain-specific interaction techniques. We present a particular DSD method, and demonstrate its use for the design of cloning techniques in a structural engineering application. Results from empirical studies demonstrate that interaction techniques produced with domain knowledge in mind outperformed other techniques by improving task efficiency, work flow, and usefulness of the 3D UI.

Download Full-text