Artificial seed production of Pangasiid catfish in the Mekong Delta, Vietnam.

Author(s):  
Thanh Phuong Nguyen

Abstract The two pangasiid catfish, Pangasius bocourti and P. hypophthalmus, are widely cultured in the Mekong Delta, Vietnam. However, seed supply until recently was dependent on fry and fingerlings caught from the Mekong River. In 1994, a research programme was carried out on artificial seed production of these two species in captive conditions. In 1995, about 30,000 fingerlings were first produced in An Giang and Can Tho provinces and a proper technique of induced breeding, progressive human chorionic gonadotropin (hCG) treatment, was determined. This technique, involving two steps of hCG injections, successfully induced ovulation in 66% of 67 treated females of P. bocourti. Application of this technique to other pangasiid catfish species such as P. conchophilus, P. hypophthalmus, P. mekongensis, and P. larnaudii also resulted in breeding success. Research on larval rearing has also been conducted to improve the growth and survival of pangasiid larvae. Extension of research findings of induced breeding and larval rearing has allowed mass seed production of pangasiid catfish in the Mekong Delta.

2014 ◽  
Vol 6 (1) ◽  
Author(s):  
Suko Ismi

Application technology for grouper seed production was conducted by dissemination activities, aimed for dissemination technology in several places having aquaculture prospects in order to facilitate seed supply. The research was conducted  in 2012 collaborated with the Department of Fisheries and Maritime Affairs Lamongan Regency and Fisheries Academy  of Sidoarjo. Application technology for grouper seed production was conducted in Tunggu village, Paciran district, Lamongan, East Java. Grouper seeds which maintained were tiger, cantik, and cantang. Seed productions were performed from egg to juvenile with size of ±3.0 cm,in which the eggs  were transported from hatchery in Bali and larval rearing was conducted based on the produced technology. The results showed that survival rate of tiger grouper was 8.3%, cantang grouper was 11,6%, and cantik grouper was 12.4%.  The results showed that R/C ratio was >1, in which the business was profitable. The technology can be applied to the community and grouper seed production  can support aquaculture activities. Keywords: Grouper seed, production, technology, R/C ratio, aquaculture


2014 ◽  
Vol 20 ◽  
pp. 99-108 ◽  
Author(s):  
MS Islam ◽  
MA Bari

Context: The application of encapsulated shoot tips and nodal segments may contribute to the protection of rare and threatened medicinal plants. Although the artificial seed technique has been reported for more than two decades, for medicinal plants this method has not been developed sufficiently. The main limitations in conventional propagation of some species with medicinal value are: reduced endosperm, low germination rate and seedless varieties. The above mentioned reasons indicate the need for the production of artificial seeds as a technique which combines the advantages of clonal multiplication with those of seed propagation and storage. Objectives: The objective of the present investigation was to standardize artificial seed production technology taking shoot tip and nodal explants in Mentha arvensis and its in vitro regeneration Materials and Methods: Sodium alginate beads were produced by encapsulation of shoot tip and nodal segments of the plant M. arvensis. MS medium was used as basal medium with agar and sodium alginate was used as gelling agent accompanied by CaCl2 solution. Results: Different concentrations and combinations of BAP, Kin and NAA were used in alginate bead in MS basal medium. Among the different concentrations of phytohormone, highest 80% of shoot formation was observed in MS medium containing 2.0 mg/l BAP + 0.2 mg/l NAA from nodal segments of M. arvensis. Highest average number of shoot 9.87 ± 0.58 formation was obtained in the same medium but highest length of shoot 6.27 ± 0.29 cm was found in the medium having 1.0 mg/l BAP + 0.5 mg/l NAA. Conclusion: The present investigation clearly established and demonstrated the method of obtaining the artificial seed production in M. arvensis supported by different hormone concentrations DOI: http://dx.doi.org/10.3329/jbs.v20i0.17722 J. bio-sci.  20:  99-108, 2012


2016 ◽  
Vol 82 (2) ◽  
pp. 151-151
Author(s):  
MAKOTO OSADA ◽  
KAZUE NAGASAWA ◽  
DAISUKE TAKAHASHI ◽  
KEISUKE TAKAHASHI ◽  
NAOKI ITOH

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