Aliphatic Nitrile Hydratase fromArthrobactersp. J-1 Purification and Characterization

1982 ◽  
Vol 46 (5) ◽  
pp. 1165-1174 ◽  
Author(s):  
Yasuhisa Asano ◽  
Kinya Fujishiro ◽  
Yoshiki Tani ◽  
Hideaki Yamada
Keyword(s):  
Nitrile Hydratase ◽  
Purification And Characterization ◽  
Aliphatic Nitrile

scholarly journals Aliphatic nitrile hydratase from Arthrobacter sp. J-1 purification and characterization.

1982 ◽  
Vol 46 (5) ◽  
pp. 1165-1174 ◽  
Author(s):  
Yasuhisa ASANO ◽  
Kinya FUJISHIRO ◽  
Yoshiki TANI ◽  
Hideaki YAMADA
Keyword(s):  
Nitrile Hydratase ◽  
Purification And Characterization ◽  
Aliphatic Nitrile

Nocardia globerula NHB-2: a versatile nitrile-degrading organism

2005 ◽  
Vol 51 (8) ◽  
pp. 705-708 ◽  
Author(s):  
Tek Chand Bhalla ◽  
Harish Kumar
Keyword(s):  
Nitrile Hydratase ◽  
Enrichment Culture ◽  
Nutrient Broth ◽  
Amidase Activity ◽  
Degrading Bacterium ◽  
Nitrilase Activity ◽  
Aliphatic Nitrile ◽  
Aromatic Amide ◽  
Aliphatic Nitriles ◽  
Hydrolysis Of

A versatile nitrile-degrading bacterium was isolated by enrichment culture from the soil of a forest near Manali, Himachal Pradesh, India, and was identified as Nocardia globerula. This organism contains 3 enzymes with nitrile-degrading activity: nitrilase, nitrile hydratase, and amidase. Nocardia globerula NHB-2 cells grown on nutrient broth supplemented with 1% glucose and 0.1% yeast extract exhibited nitrile hydratase–amidase activity specific for saturated aliphatic nitriles or amide, while addition of acetonitrile in nutrient broth yielded cells with nitrile hydratase–amidase that in addition to saturated aliphatic nitriles–amide also hydrolyzed aromatic amide. Nocardia globerula NHB-2 cultivated on nutrient broth containing propionitrile exhibited nitrilase activity that hydrolyzed aromatic nitrile and unsaturated aliphatic nitrile. The versatility of this organism in the hydrolysis of various nitriles and amides makes it a potential bioresource for use in organic synthesis.Key words: Nocardia globerula NHB-2, nitrilase, nitrile hydratase, amidase, nitrile–amide degradation.

scholarly journals Nitrile hydratase of Pseudomonas chlororaphis B23. Purification and characterization

1987 ◽  
Vol 162 (3) ◽  
pp. 691-698 ◽  
Author(s):  
Toru NAGASAWA ◽  
Hironori NANBA ◽  
Koichiro RYUNO ◽  
Koji TAKEUCHI ◽  
Hideaki YAMADA
Keyword(s):  
Nitrile Hydratase ◽  
Pseudomonas Chlororaphis ◽  
Purification And Characterization

Purification and characterization of the enantioselective nitrile hydratase fromRhodococcus sp. AJ270

2007 ◽  
Vol 2 (6) ◽  
pp. 717-724 ◽  
Author(s):  
Liya Song ◽  
Meixiang Wang ◽  
Xiuqing Yang ◽  
Shijun Qian
Keyword(s):  
Nitrile Hydratase ◽  
Purification And Characterization

Chaperone-assisted expression, purification, and characterization of recombinant nitrile hydratase NI1 from Comamonas testosteroni

2003 ◽  
Vol 29 (1) ◽  
pp. 70-76 ◽  
Author(s):  
Julie M. Stevens ◽  
Narsing Rao Saroja ◽  
Maryse Jaouen ◽  
Maya Belghazi ◽  
Jean-Marie Schmitter ◽  
...  
Keyword(s):  
Nitrile Hydratase ◽  
Comamonas Testosteroni ◽  
Purification And Characterization
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