Cross-Linking Interferes with Assessing Sulfur Mustard-Induced DNA Damage in Human Peripheral Blood Lymphocytes Using the Comet Assay

2004 ◽  
Vol 14 (3) ◽  
pp. 195-202 ◽  
Author(s):  
Janet Moser ◽  
Claire F. Levine ◽  
Delvena R. Thomas-Dunmeyer ◽  
William J. Smith
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Peripheral Blood ◽  
Sulfur Mustard ◽  
Human Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Cross Linking ◽  
Blood Lymphocytes ◽  
Human Peripheral Blood Lymphocytes

scholarly journals Genotoxic effects of diazinon on human peripheral blood lymphocytes / Genotoksično djelovanje diazinona na limfocite ljudske periferne krvi

2015 ◽  
Vol 66 (2) ◽  
pp. 153-158 ◽  
Author(s):  
Fulya Dilek Gökalp Muranli ◽  
Martin Kanev ◽  
Kezban Ozdemir
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Human Lymphocytes ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes ◽  
Human Peripheral Blood Lymphocytes ◽  
Current Assessment ◽  
High Level

Abstract The aim of this study was to evaluate the genetic damage in human peripheral blood lymphocytes following 24 and 48- hour exposure to a commercial diazinon formulation Basudin 60EM® at concentrations between 0.01 and 40 μg mL-1. For this purpose we used the micronucleus (MN), fluorescence in situ hybridization (FISH), and alkaline single cell gel electrophoresis (comet) assay. Diazinon significantly increased the frequency of micronucleated cells compared to control. Forty-eight-hour exposure increased this frequency even at lower concentrations (0.01-10 μg mL-1). The FISH results revealed aneugenic effects at 10 μg mL-1. The comet assay also confirmed DNA damage at concentrations between 10 and 40 μg mL-1. Our findings have confirmed the genotoxic potential of diazinon and its cytotoxic effect on human lymphocytes. The increased DNA damage in our study raises concern about the current assessment of the health risk posed by this pesticide and calls for a high level of caution in agricultural and household use.

scholarly journals Evaluation of Genotoxic and Cytotoxic Effects in Human Peripheral Blood Lymphocytes ExposedIn Vitroto Neonicotinoid Insecticides News

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
María Elena Calderón-Segura ◽  
Sandra Gómez-Arroyo ◽  
Rafael Villalobos-Pietrini ◽  
Carmen Martínez-Valenzuela ◽  
Yolanda Carbajal-López ◽  
...  
Keyword(s):  
Dna Damage ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Blue Dye ◽  
Cytotoxic Effects ◽  
Blood Lymphocytes ◽  
Neonicotinoid Insecticides ◽  
Human Peripheral Blood Lymphocytes

Calypso (thiacloprid), Poncho (clothianidin), Gaucho (imidacloprid), and Jade (imidacloprid) are commercial neonicotinoid insecticides, a new class of agrochemicals in México. However, genotoxic and cytotoxic studies have not been performed. In the present study, human peripheral blood lymphocytes (PBL) were exposedin vitroto different concentrations of the four insecticides. The genotoxic and cytotoxic effects were evaluated using the alkaline comet and trypan blue dye exclusion assays. DNA damage was evaluated using two genotoxicity parameters: tail length and comet frequency. Exposure to9.5×10-6to5.7×10-5 M Jade;2.8×10-4to1.7×10-3 M Gaucho;0.6×10-1to1.4×10-1 M Calypso;1.2×10-1to9.5×10-1 M Poncho for 2 h induced a significant increase DNA damage with a concentration-dependent relationship. Jade was the most genotoxic of the four insecticides studied. Cytotoxicity was observed in cells exposed to18×10-3 M Jade,2.0×10-3 M Gaucho,2.0×10-1 M Calypso, 1.07 M Poncho, and cell death occurred at30×10-3 M Jade,3.3×10-3 M Gaucho,2.8×10-1 M Calypso, and 1.42 M Poncho. This study provides the first report of genotoxic and cytotoxic effects in PBL followingin vitroexposure to commercial neonicotinoid insecticides.

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