scholarly journals A novel intermediate filament-associated protein, NAPA-73, that binds to different filament types at different stages of nervous system development.

1986 ◽  
Vol 102 (1) ◽  
pp. 246-251 ◽  
Author(s):  
G Ciment ◽  
A Ressler ◽  
P C Letourneau ◽  
J A Weston
Keyword(s):  
Monoclonal Antibody ◽  
Intermediate Filament ◽  
System Development ◽  
Cell Types ◽  
Embryonic Cell ◽  
Nervous System Development ◽  
Intermediate Filament Protein ◽  
Mature Neurons ◽  
Filament Protein ◽  
Vimentin Intermediate Filament

The antigen recognized by the E/C8-monoclonal antibody is expressed in various avian embryonic cell types known also to express neurofilament (NF) immunoreactivity. To determine whether the E/C8-antigen corresponds to any of the known NF components, we compared their subcellular locations, immunocross-reactivities, and electrophoretic behaviors. We found that the E/C8-antibody binds to NF bundles in electron microscope preparations of neurons, but does not correspond to any of the known NF proteins by immunological or electrophoretic criteria. Immunoadsorption with the monoclonal antibody resulted in co-purification of a 73,000-D protein with one of the known NF proteins in homogenates from 20-d embryonic chick brains, but with vimentin intermediate filament protein in similarly prepared homogenates from 4-d embryonic chicks. We suggest that the E/C8-antigen is an intermediate filament-associated protein that binds to different filament types at different stages of development. We have named it NAPA-73, an acronym for neurofilament-associated protein, avian-specific, 73,000 D, on the basis of its binding specificity in mature neurons.

scholarly journals A Monoclonal Antibody Recognizes a Form of Intermediate Filament Protein in Rat Sertoli Cells That Is Not Present in Seminiferous Peritubular Cells1

1986 ◽  
Vol 35 (1) ◽  
pp. 227-238 ◽  
Author(s):  
Abraham L. Kierszenbaum ◽  
James A. Crowell ◽  
Robert B. Shabanowitz ◽  
Eric P. Smith ◽  
W. Austin Spruill ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Sertoli Cells ◽  
Intermediate Filament ◽  
Intermediate Filament Protein ◽  
Filament Protein

scholarly journals Reexpression of glial fibrillary acidic protein rescues the ability of astrocytoma cells to form processes in response to neurons.

1994 ◽  
Vol 127 (3) ◽  
pp. 813-823 ◽  
Author(s):  
W J Chen ◽  
R K Liem
Keyword(s):  
Glial Fibrillary Acidic Protein ◽  
Intermediate Filament ◽  
Nervous System Development ◽  
Acidic Protein ◽  
Stable Processes ◽  
Intermediate Filament Protein ◽  
Granule Neurons ◽  
Astrocytoma Cells ◽  
Filament Protein ◽  
U251 Cells

Astroglial cells play an important role in orchestrating the migration and positioning of neurons during central nervous system development. Primary astroglia, as well as astrocytoma cells will extend long stable processes when co-cultured with granule neurons. In order to determine the function of the glial fibrillary acidic protein (GFAP), the major intermediate filament protein in astroglia and astrocytoma cells, we suppressed the expression of GFAP by stable transfection of an anti-sense GFAP construct in human astrocytoma U251MG cells. The resulting AS2-U251 cells can no longer extend stable processes in the presence of granule neurons. To show that this effect is due specifically to the absence of GFAP, we reintroduced a fully encoding rat brain GFAP cDNA into these AS2-U251 cells. The resulting rat GFAP appeared as a filamentous network and the reexpression of GFAP rescued the ability of these astrocytoma cells to form stable processes when co-cultured with neurons. From these results, it is clear that the glial specific intermediate filament protein, GFAP, is required for process extension of these astrocytoma cells in response to granule neurons.

Vimentin intermediate filaments in fish melanophores

1987 ◽  
Vol 88 (5) ◽  
pp. 649-655
Author(s):  
F.K. Gyoeva ◽  
E.V. Leonova ◽  
V.I. Rodionov ◽  
V.I. Gelfand
Keyword(s):  
Intermediate Filaments ◽  
Intermediate Filament ◽  
Polyclonal Antibodies ◽  
Cell Types ◽  
Immunofluorescence Staining ◽  
Intermediate Filament Protein ◽  
Transmission Electron ◽  
Filament Protein ◽  
Pterophyllum Scalare ◽  
Cell Centre

The distribution and chemical composition of intermediate filaments in cultured melanophores of two teleost species - Gymnocorymbus ternetzi and Pterophyllum scalare - were studied by immunofluorescence staining and immunoblotting techniques. The immunofluorescence staining of the melanophores with monoclonal and polyclonal antibodies to the intermediate filament protein vimentin revealed a system of fibrils radiating from the cell centre. These fibrils were resistant to 0.6 M-KCl and nocodazole treatments as has been found in other cell types. Transmission electron microscopy confirmed the presence of intermediate filaments in melanophores. Immunoblotting experiments showed the presence of the intermediate filament protein vimentin in melanophore lysates. Therefore, teleost melanophores possess a developed radial system of vimentin intermediate filaments.

scholarly journals Phosphorylation of the peripherin 58-kDa neuronal intermediate filament protein

1989 ◽  
Vol 264 (8) ◽  
pp. 4619-4627
Author(s):  
J M Aletta ◽  
M L Shelanski ◽  
L A Greene
Keyword(s):  
Intermediate Filament ◽  
Intermediate Filament Protein ◽  
Filament Protein
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