Molecular Characterization of South American Bovine Herpesvirus-1 Isolates with Monoclonal Antibodies and SDS-PAGE

1993 ◽  
Vol 40 (1-10) ◽  
pp. 125-130 ◽  
Author(s):  
A. Suarez Heinlein ◽  
A. E. Metzler ◽  
R. Weiblen ◽  
P. Berrios ◽  
A. A. Schudel ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Molecular Characterization ◽  
Bovine Herpesvirus ◽  
South American ◽  
Bovine Herpesvirus 1 ◽  
Sds Page ◽  
Herpesvirus 1

scholarly journals First Report on the Detection and Molecular Characterization of Bovine Herpesvirus 1 from a Clinical case of Infectious Bovine Rhinotracheitis in Pakistan

2021 ◽  
Vol 41 (01) ◽  
pp. 160-162
Author(s):  
Aayesha Riaz
Keyword(s):  
Molecular Characterization ◽  
Nested Pcr ◽  
Bovine Herpesvirus ◽  
Nasal Discharge ◽  
Infectious Bovine Rhinotracheitis ◽  
Bovine Herpesvirus 1 ◽  
Nasal Swabs ◽  
Herpesvirus 1 ◽  
First Time

Bovine herpesvirus 1 (BHV-1) is a noteworthy reason for many Cattle/Buffalo diseases. Infectious bovine rhinotracheitis (IBR) is one of the diseases which are caused by the BHV-1. In the present study a cow which was suspected of IBR was examined. The animal was suffering from fever and respiratory distress along with rhinitis (red nose), in appetence, and dyspnea. The nasal mucosa and muzzle were distinctly inflamed, with nasal discharge. DNA from blood samples and nasal swabs were subjected to nested PCR using glycoprotein B gene (gB) Primers. The samples were found positive for BHV-1 gB gene. Sequencing and phylogenetic analysis revealed close similarities with other BHV-1 gB gene sequences. The accession numbers assigned to this pioneer sequences in GenBank are MT449510 (BHV-1-Pak 1) and MT449511 (BHV-1-Pak 2). In this study, we reported for the first time the detection of DNA of BHV-1 through nested PCR assay and molecular characterization of BHV-1 gB gene in Pakistan. This study will be useful in further diagnoses of BHV-1 in Pakistan and in development of BHV-1 vaccine to reduce economical losses due to IBR

Characterization of monoclonal antibodies against bovine herpesvirus 1 gD fusion protein expressed in E. coli

1999 ◽  
Vol 83 (1-2) ◽  
pp. 83-89 ◽  
Author(s):  
Japhet R.S Lyaku ◽  
Patricia K McKenna ◽  
Robert A Fredrickson ◽  
Frederick S.B Kibenge
Keyword(s):  
Monoclonal Antibodies ◽  
Fusion Protein ◽  
Bovine Herpesvirus ◽  
Bovine Herpesvirus 1 ◽  
E Coli ◽  
Herpesvirus 1

Phylogenetic analysis and characterization of bovine herpesvirus-1 in cattle of China, 2016–2019

2020 ◽  
Vol 85 ◽  
pp. 104416 ◽  
Author(s):  
Yulong Zhou ◽  
Xuyang Li ◽  
Yachao Ren ◽  
Xilin Hou ◽  
Yu Liu ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Bovine Herpesvirus ◽  
Bovine Herpesvirus 1 ◽  
Herpesvirus 1

scholarly journals Detection of Bovine Herpesvirus-1-Specific IgM Using a Capture Enzyme Immune Assay with Isotype-Specific Monoclonal Antibodies

1989 ◽  
Vol 1 (2) ◽  
pp. 139-145 ◽  
Author(s):  
Fernando Osorio ◽  
Subramaniam Srikumaran ◽  
Marvin Rhodes ◽  
David Christensen ◽  
Pushpa Srikumaran
Keyword(s):  
Monoclonal Antibodies ◽  
Solid Phase ◽  
Bovine Herpesvirus ◽  
Immunoglobulin M ◽  
Viral Reactivation ◽  
Clinical Samples ◽  
Serum Samples ◽  
Bovine Herpesvirus 1 ◽  
Igm Antibody ◽  
Herpesvirus 1

The detection of virus-specific immunoglobulin M (IgM) antibodies in acute-phase serum samples offers the possibility of making an accurate and rapid serologic diagnosis. We have developed a solid-phase capture assay that uses murine monoclonal antibodies specific for bovine IgM to separate the whole IgM fraction of a bovine serum sample. The IgM specific for bovine herpesvirus-1 (BHV-1) is then detected by the addition of viral antigen, which in turn is detected by BHV-1-specific monoclonal antibodies conjugated to horseradish peroxidase. A BHV-1 IgM antibody response was detected during the early postinfection period (7–40 days PI). Bovine herpesvirus-1 IgM antibody was not detected in sera taken from 3 animals following dexamethasone-induced viral reactivation. This method compares favorably with viral isolation, antigen detection in the clinical samples, and paired serology in the diagnosis of BHV-1 infection at a herd level.

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