Serological and molecular characterization of mouse anti-idiotypic monoclonal antibodies elicited with the syngeneic Anti-HLA-A2,28 monoclonal antibody CR11-351

SummaryFor identification of lipopolysaccharide (LPS)-associated epitopes ofLegionella pneumophilaserogroup 1, LPS of strain Philadelphia 1 was investigated using monoclonal antibodies (MAbs). The O-specific chain of LPS is a homopolymer of 5-acetamidino-7-acetamido-8-O-acetyl-3,5,7,9-tetradeoxy-D-glycero-L-galacto-nonulosonic acid. At least four immunoaccessible epitopes were recognized by different MAbs on the intact LPS. AfterO-deacetylation of LPS, the reactivity of one of the MAbs (MAb 3/1) was lost, indicating thus that the corresponding epitope is associated with the 8-O-acetyl group. Since the reactivity pattern of the MAb 3/1 is identical with those of the MAb 2 which was considered as a virulence marker for serogroup 1, this epitope may be involved in mediating virulence inL. pneumophila. Four MAbs specific to strains of serogroup 1 other than the monoclonal subtype Philadelphia recognized epitopes on theO-deacetylated LPS of strain Philadelphia 1 and, therefore, the virulence-associated epitope blocks recognition of the immunodeterminants that are accessible on the intact LPS of the strains lacking this epitope.

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Production and characterization of monoclonal antibodies to the edta extract of Leptospira interrogans, serovar icterohaemorrhagiae

Revista da Sociedade Brasileira de Medicina Tropical ◽

10.1590/s0037-86821996000500012 ◽

1996 ◽

Vol 29 (5) ◽

pp. 483-489

Author(s):

Lilian Terezinha de Queiroz Leite ◽

Mauricio Resende ◽

Wanderley de Souza ◽

Elizabeth R.S. Camargos ◽

Matilde Cota Koury

Keyword(s):

Monoclonal Antibody ◽

Monoclonal Antibodies ◽

Western Blot ◽

Silver Staining ◽

Colloidal Gold ◽

Leptospira Interrogans ◽

Outer Envelope ◽

Lethal Challenge ◽

Sds Page

Monoclonal antibodies (MABs) ivere produced against an etbylenediaminetetraacetate (EDTA) extract of Leptospira interrogans serovar icterohaemorrhagiae being characterized by gel precipitation as IgM and IgG (IgGl and IgG2b). The EDTA extract was detected as several bands by silver staining in SDS-PAGE. In the Western blot the bands around 20 KDa reacted with a monoclonal antibody, 47B4D6, and was oxidized by periodate and was not digested by pronase, suggesting that the determinant is of carbohydrate nature, lmmunocytochemistry, using colloidal gold labeling, showed that an EDTA extract determinant recognized by monoclonal antibody 47B4D6, is localized under the outer envelope of serovar icterohaemorrhagiae. Hoe AIAB raised against the EDTA extract was not able to protect hamsters from lethal challenge with virulent homologous leptospires.

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Molecular Characterization of Variable Heavy and Light Chain Regions of Five HIV Type 1-Specific Human Monoclonal Antibodies*

AIDS Research and Human Retroviruses ◽

10.1089/aid.1994.10.1639 ◽

1994 ◽

Vol 10 (12) ◽

pp. 1639-1649 ◽

Cited By ~ 11

Author(s):

ERIC M.M. van der DONK ◽

MARTIN SCHUTTEN ◽

ALBERT D.M.E. OSTERHAUS ◽

ROGER W.J. van der HEIJDEN

Keyword(s):

Monoclonal Antibodies ◽

Molecular Characterization ◽

Light Chain ◽

Human Monoclonal Antibodies ◽

Variable Heavy ◽

Hiv Type 1

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Isolation of monoclonal antibodies specific for products of avian oncogene myb.

Molecular and Cellular Biology ◽

10.1128/mcb.4.12.2843 ◽

1984 ◽

Vol 4 (12) ◽

pp. 2843-2850 ◽

Cited By ~ 28

Author(s):

G I Evan ◽

G K Lewis ◽

J M Bishop

Keyword(s):

Monoclonal Antibody ◽

Monoclonal Antibodies ◽

Nuclear Protein ◽

Gene Products ◽

Viral Oncogenes ◽

Myb Gene

We isolated a series of monoclonal antibodies which were raised against a bacterially expressed protein, bp37v-myb, and coded for by part of the avian v-myb gene. These monoclonal antibodies recognized a range of antigenic specificities on bp37v-myb, and this was reflected in their differing specificities for the gene products of the v-myb, c-myb, and E26 viral oncogenes. One monoclonal antibody recognized, in addition to the v-myb and c-myb gene products, a conserved nuclear protein found in all tested cells. We describe the characterization of these monoclonal antibodies.

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Generation and Characterization of a Murine Monoclonal Antibody Specific for the Human T1-Cd5 Molecule

The International Journal of Biological Markers ◽

10.1177/172460088700200302 ◽

1987 ◽

Vol 2 (3) ◽

pp. 143-150 ◽

Cited By ~ 2

Author(s):

Federico Genzano ◽

Ada Funaro ◽

Massimo Alessio ◽

Lucia B. De Monte ◽

Graziella Bellone ◽

...

Keyword(s):

Monoclonal Antibody ◽

Monoclonal Antibodies ◽

T Cell ◽

T Lymphocytes ◽

Membrane Glycoprotein ◽

Functional Features ◽

Specific Membrane ◽

Murine Monoclonal Antibodies ◽

Selection Of

Murine monoclonal antibodies (MoAbs) have found widespread applications in the characterization of the molecular and functional features of lymphocyte differentiation antigens. The present paper summarizes the results of our work dealing with the production and selection of a murine MoAb recognizing a molecule expressed during the whole differentiative life of T lymphocytes. The MoAb CB01 resulted to be specific for an apparently unique epitope of the T-cell specific membrane glycoprotein T1-CD5.

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