Effects of Pressure, Temperature, Adsorbent Surface, and Mobile Phase Composition on the Supercritical Fluid Chromatographic Fractionation of Monodisperse Polystyrenes

1978 ◽  
Vol 16 (3) ◽  
pp. 102-110 ◽  
Author(s):  
J. E. Conaway ◽  
J. A. Graham ◽  
L. B. Rogers
Keyword(s):  
Phase Composition ◽  
Supercritical Fluid ◽  
Mobile Phase ◽  
Mobile Phase Composition ◽  
Chromatographic Fractionation ◽  
Adsorbent Surface

Effect of the Mobile Phase Composition on Selectivity in Supercritical Fluid Chromatography in the Separation of Salbutamol Enantiomers

2018 ◽  
Vol 12 (7) ◽  
pp. 1166-1175 ◽  
Author(s):  
M. O. Kostenko ◽  
K. B. Ustinovich ◽  
O. I. Pokrovskiy ◽  
O. O. Parenago ◽  
N. G. Bazarnova ◽  
...  
Keyword(s):  
Phase Composition ◽  
Supercritical Fluid ◽  
Supercritical Fluid Chromatography ◽  
Mobile Phase ◽  
Mobile Phase Composition

scholarly journals Influence of mobile phase composition and temperature on the retention behavior of selected test substances in diol-type column

2020 ◽  
pp. 72-81
Author(s):  
Wojciech Zapała ◽  
Piotr Ziobrowski ◽  
Lidia Zapała ◽  
Marcin Chutkowski
Keyword(s):  
Phase Composition ◽  
Mobile Phase ◽  
Surface Heterogeneity ◽  
Water Systems ◽  
Retention Mechanism ◽  
Retention Behavior ◽  
Mobile Phase Composition ◽  
Mode Water ◽  
Hydrogen Bond Interactions ◽  
Adsorbent Surface

In this work the effect of mobile phase composition and temperature on the retention behavior of several test compounds (including non-polar and polar substances) on diol-Acclaim Mixed Mode HILIC-1 column in methanol-water systems with high (RPLC mode) and low (HILIC mode) water content was analyzed. In the studied systems both linear, slightly curved and curved van’t Hoff plots were observed. This indicates, that depending on solute physicochemical properties and mobile phase composition, both single and more complex retention mechanism(s) may influence the retention behavior of tested substances. The possibilities of different retention mechanisms existence (e.g. connected with adsorbent surface heterogeneity, hydrophobic and hydrogen bond interactions) in the analyzed systems have been also discussed.

Sequential Injection Chromatography with Monolithic Column for Phenothiazines Assay in Human Urine and Pharmaceutical Formulations

2020 ◽  
Vol 16 (7) ◽  
pp. 967-975
Author(s):  
Abubakr M. Idris
Keyword(s):  
Phase Composition ◽  
Human Urine ◽  
Mobile Phase ◽  
Monolithic Column ◽  
Pharmaceutical Formulations ◽  
Pharmaceutical Formulation ◽  
Assay Method ◽  
Sequential Injection ◽  
Mobile Phase Composition ◽  
Sequential Injection Chromatography

Methods: Sequential injection chromatography (SIC) with monolithic column has been proposed with potential benefits for separation and quantification. Objective: To utilize SIC to develop a new assay method for the separation and quantification of some phenothiazines (promethazine, chlorpromazine and perphenazine) in human urine and synthetic pharmaceutical formulations. Methods: The 32 full-factorial design was adopted to study the effect of mobile phase composition on separation efficiency, retention time, peak height and baseline. The separation was conducted on a C18 monolithic column (100 × 4.6 mm) using a mobile phase composition of phosphate: acetonitrile:methanol (60:28:12) at pH 4.0. The detection was carried out using a miniaturized fiber optic spectrometer at 250 nm. Results: Satisfactory analytical features, including number of theoretical plates (1809-6232), peak symmetry (1.0-1.3), recovery (95.5-99.1% in pharmaceutical formulations and 91.6-94.7% in urine), intra-day precision (0.36-1.60% for pharmaceutical formulation and 2.96-3.67 for urine), inter-day precision (1.47-2.28% for pharmaceutical formulation), limits of detection (0.23-0.88 μg/ml) and limits of quantification (0.77-2.90 μg/ml), were obtained. Conclusion: The remarkable advantages of the proposed SIC method are the inexpensiveness in terms of instrumentation and reagent consumption.

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