Preparation of poly (ionic liquid@MOF) composite monolithic column and its application in online enrichment and purification of tectochrysin in medicinal plants

In this paper, a poly (ionic liquid@MOF) composite monolithic column was prepared by in situ radical polymerization using ionic liquid (1-allyl-3-methylimidazolium hexafluorophosphate) and MOF (derivatized UIO66-2COOH) as copolymer monomers. The...

Eco-Friendly Separation of Antihyperlipidemic Combination Using UHPLC Particle-Packed and Monolithic Columns by Applying Green Analytical Chemistry Principles

Efficient separation of pharmaceuticals and metabolites with the adequate resolution is a key factor in choosing the most suitable chromatographic method. For quality control, the analysis time is a key factor, especially in pharmacokinetic studies. High back pressure is considered as one of the most important factors in chromatography’s flow control, especially in UHPLC. The separation of the anti-hyperlipidemic mixtures was carried out using two columns: a column silica-based particle packed UHPLC and a monolithic column. The systematic suitability of the two columns was compared for the separation of Fenofibrate, its active metabolite, Fenofibric acid and Pravastatin using Atorvastatin as an internal standard. Separation on both columns was obtained using ethanol: buffer potassium dihydrogen orthophosphate pH = 3 (adjusted with orthophosphoric acid) (75:25 v/v) as mobile phase and flow rate 0.8 mL/min. The analytes’ peak detection was achieved by using a PDA detector at 287 nm, 214 nm, 236 nm, and 250 nm for Fenofibrate, Fenofibric acid, Pravastatin, and Atorvastatin, respectively. Reduction of back-pressure was achieved with the monolithic column, where the analytes could be completely separated in less than 1.5 min at a flow rate of 5 mL/min. The principles of Green Analytical Chemistry (GAC) were followed throughout the developed method using environmentally safe solvents.

Analysis on the value of stiffness of the dual joint straightness notch joint of beams due to cyclic lateral load

Precast concrete is an answer to the demands of building structures that save time, but cannot be used widely because of the reliability of the connection, especially during an earthquake, the desired earthquake-resistant building structure must have sufficient strength and rigidity. Stiffness is one of the factors that determine the response of a structure to earthquake loads. When connected with earthquake loads, a structure must have sufficient rigidity so that its movement during an earthquake can be limited. This study aims to determine and analyze the stiffness in the double columns straight joint beam notches due to lateral cyclic load. By dividing 3 (three) types of test specimens, namely Monolithic column Beam, Type 1 Column Joint (SBK), and Type 2 Column Beam Joint (SBK). The connection used is a double straight notch and using the grouting method. Testing and analysis using the Displacement Control Method with the European Convention for Constructional Steelwork (ECCS) 1986 standards. The results showed the monolith column Column (BK) specimens have a greater stiffness value compared to SBK 1 specimens and SBK 2 specimens.

Silica Microspheres-in-Pores Composite Monoliths with Fluorescence and Potential for Water Remediation

Water pollution is a severe worldwide issue. Constructing advanced porous composite materials has been an efficient route to water remediation via adsorption. In this study, a unique microspheres-in-pores monolithic structure was fabricated. An emulsion-templated polymer monolith was first prepared and silica microspheres were subsequently formed in the porous polymer. A silica precursor was modified with a fluorescent dye and co-condensed with other precursors to fabricate porous composites with fluorescent properties, which were enhanced by the presence of Ag nanoparticles in the polymer matrix. This unique material showed good promise in water remediation by removing organic dyes and heavy metal ions from wastewater via a flowing filter or monolithic column separation.

CALCULATION OF A MONOLITHIC COLUMN FOUNDATION FOR A REINFORCED CONCRETE COLUMN OF A MULTI-STOREY BUILDING IN LVIV

Problem statement. In modern construction of residential and commercial buildings, bridges and other structures, columns are often the main load-bearing elements. Different in the way they are manufactured and in their characteristics, these building elements serve as the basis of the framework on which all other building structures are placed. However, in order to have a strong, durable and, most importantly, correct construction of the whole structure, the columns should be installed with minimum deviations from the design values. This is why a great deal of attention is paid to the foundations in the design and implementation of the project. The purpose of the article is to develop a calculation of a monolithic column foundation for a reinforced concrete column of a multi-storey building in Lviv based on theoretical research. The design is based on theoretical studies and is based on the design of a monolithic column foundation for a multi-storey building in Lvov, taking into account the dimensions of the foundation, the soil characteristics, the self-weight of the foundation and the longitudinal reinforcement coefficient. Analysis of publications. Studying not only foreign, but also domestic current theoretical developments and practical experience will allow the objective to be achieved. There are examples among European countries that largely meet the current requirements. Results of the study. The most common soils in Lviv are chernozem, eluvial and peat-bog soils. The climate in Lviv is moderately continental with mild winters and warm summers. With these data, the dimensions of the monolithic columnar foundation for a multi-storey building are calculated and its reinforcement is determined. The strength of the monolithic columnar foundation for the reinforced concrete column of the multi-storey building is tested for the punching strength. Based on the results of the calculations, it is determined that the foundation is sufficiently strong under the column.Conclusions. In this article, the characteristics of soils and climatic conditions of the construction area in the city of Lviv were investigated. The dimensions of the foundation's underside, all dimensions of the foundation and the pressure on the ground under the underside from the design load were calculated. The reinforcement area of the foundation footing was selected and the strength of the foundation was tested for push-through.

Recent Advances about the Applications of Click Reaction in Chemical Proteomics

Despite significant advances in biological and analytical approaches, a comprehensive portrait of the proteome and its dynamic interactions and modifications remains a challenging goal. Chemical proteomics is a growing area of chemical biology that seeks to design small molecule probes to elucidate protein composition, distribution, and relevant physiological and pharmacological functions. Click chemistry focuses on the development of new combinatorial chemical methods for carbon heteroatom bond (C-X-C) synthesis, which have been utilized extensively in the field of chemical proteomics. Click reactions have various advantages including high yield, harmless by-products, and simple reaction conditions, upon which the molecular diversity can be easily and effectively obtained. This paper reviews the application of click chemistry in proteomics from four aspects: (1) activity-based protein profiling, (2) enzyme-inhibitors screening, (3) protein labeling and modifications, and (4) hybrid monolithic column in proteomic analysis.