Measurement of Total Body Hemoglobin by Bloodless Technic

1967 ◽  
Vol 13 (10) ◽  
pp. 877-889 ◽  
Author(s):  
Bernard J Katchman ◽  
James P F Murphy ◽  
Kenneth M Offner ◽  
Jerome C Fox
Keyword(s):  
Carbon Monoxide ◽  
Serum Albumin ◽  
Correlation Coefficient ◽  
Blood Volume ◽  
Continuous Monitoring ◽  
Total Body ◽  
Infrared Analyzer

Abstract The theory and methodology for the determination of total body hemoglobin by Sjostrand's closed breathing technic has been evaluated. The results show that it is possible to determine total body hemoglobin from the measurement of alveolar carbon monoxide concentrations. A system for closed breathing measurement was designed and assembled which permits continuous monitoring of alveolar carbon monoxide and oxygen. Improvements in this system are suggested such that total body hemoglobin may be read out directly, from the determination of alveolar carbon monoxide concentrations, by means of a sensitive infrared analyzer. Such measurements of total body hemoglobin have a high correlation coefficient with measurements of total body hemoglobin made by the Risa (radioiodinated serum albumin) blood volume technic.

scholarly journals 3092 Measuring Fluid Compartments Before and After Rapid Saline Infusion

2019 ◽  
Vol 3 (s1) ◽  
pp. 48-49
Author(s):  
Kevin Lawrence Kelly ◽  
Alex R. Carlson ◽  
Bradley B. Cierzan ◽  
Jennifer Isautier ◽  
Wayne L. Miller ◽  
...  
Keyword(s):  
Carbon Monoxide ◽  
Blood Volume ◽  
Total Body Water ◽  
Bioelectrical Impedance ◽  
Impedance Analysis ◽  
Body Water ◽  
Rapid Infusion ◽  
Before And After ◽  
Fluid Compartments ◽  
Total Body

OBJECTIVES/SPECIFIC AIMS: To evaluate the ability of various techniques to track changes in body fluid volumes before and after a rapid infusion of saline. METHODS/STUDY POPULATION: Eight healthy participants (5M; 3F) completed baseline measurements of 1) total body water using ethanol dilution and bioelectrical impedance analysis (BIA) and 2) blood volume, plasma volume and red blood cell (RBC) volume using carbon monoxide rebreathe technique and I-131 albumin dilution. Subsequently, 30mL saline/kg body weight was administered intravenously over 20 minutes after which BIA and ethanol dilution were repeated. RESULTS/ANTICIPATED RESULTS: On average, 2.29±0.35 L saline was infused with an average increase in net fluid input-output (I/O) of 1.56±0.29 L. BIA underestimated measured I/O by −3.4±7.9%, while ethanol dilution did not demonstrate a measurable change in total body water. Carbon monoxide rebreathe differed from I-131 albumin dilution measurements of blood, plasma and RBC volumes by +0.6±2.8%, −5.4±3.6%, and +11.0±4.7%, respectively. DISCUSSION/SIGNIFICANCE OF IMPACT: BIA is capable of tracking modest changes in total body water. Carbon monoxide rebreathe appears to be a viable alternative for the I-131 albumin dilution technique to determine blood volume. Together, these two techniques may be useful in monitoring fluid status in patients with impaired fluid regulation.

scholarly journals Ultrathinfilm Immunoelectrophoresis

1982 ◽  
Vol 19 (2) ◽  
pp. 114-116
Author(s):  
Anne Michiak ◽  
Penelope M S Clark ◽  
Larry J Kricka
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Correlation Coefficient ◽  
Chemical Method ◽  
Agarose Gels ◽  
Model Study ◽  
Speed Of Analysis

Immunoelectrophoresis (Laurell-rocket) in ultrathinfilm gels has been applied to the determination of human serum albumin in a model study. Agarose gels of 0·24 mm thickness were used and optimum electrophoretic conditions were determined. Precision was assessed and comparison with conventional immunoelectrophoresis and a chemical method was made. Though precision was poor (CV 4–6%) improvements in dispensing small volumes (0·2 μl) should change this. Good correlation between methods (correlation coefficient 0·89–0·97) was obtained. The advantages of the technique are speed of analysis and low reagent costs.

Sign in / Sign up

Export Citation Format

Share Document