Determination of Calcium in Urine and Serum by Atomic Absorption Spectrophotometry (AAS)

1967 ◽  
Vol 13 (2) ◽  
pp. 101-114 ◽  
Author(s):  
David L Trudeau ◽  
Esther F Freier
Keyword(s):  
Atomic Absorption ◽  
Atomic Absorption Spectrophotometry ◽  
Test Method ◽  
Double Blind ◽  
Absorption Spectrophotometry ◽  
Reference Methods ◽  
Free Serum ◽  
Urine And Serum

Abstract The determination of calcium in biologic fluids by atomic absorption spectrophotometry is interfered with by the presence of protein, cations, and those anions that form complexes with calcium. Such interference was overcome when lanthanum was included in a 1:50 dilution of serum or urine. Recovery of calcium added to calcium-free serum was 100%. The S.D. based on double-blind duplicates was 0.22 mg./lOO ml. Excellent statistical agreement was found between the test method and each of the 2 reference methods.

Lead in Evaporated Milk by Anodic Stripping Voltammetry and Atomic Absorption Spectrophotometry: Cooperative Interlaboratory Study

1978 ◽  
Vol 61 (4) ◽  
pp. 931-936
Author(s):  
Arlene M Sulek ◽  
Edgar R Elkins ◽  
Eric W Zink
Keyword(s):  
Atomic Absorption ◽  
Anodic Stripping Voltammetry ◽  
Lead Level ◽  
Stripping Voltammetry ◽  
Atomic Absorption Spectrophotometry ◽  
Double Blind ◽  
Absorption Spectrophotometry ◽  
Anodic Stripping ◽  
Normal Variability

Abstract The results of a cooperative study on the determination of lead in evaporated milk, using a double blind referee technique, are reported. This study was designed to determine the normal variability of methods currently used for lead analysis by canned food industry laboratories. Twenty-three laboratories participated in this study. Each laboratory was instructed to use atomic absorption spectrophotometry (AOAC 25.065), anodic stripping voltammetry, or carbon rod atomic absorption spectrophotometry. Overall, the results appear to be in close agreement with the spiking levels. The coefficient of variation for all laboratories was 36.0% at the 0.15 ppm lead level and 16.8% at the 0.40 ppm lead level.

Determination of Minor Nutrients in Fertilizers by Atomic Absorption Spectrophotometry: Second Collaborative Study

1965 ◽  
Vol 48 (6) ◽  
pp. 1100-1103
Author(s):  
C H Mcbride
Keyword(s):  
Atomic Absorption ◽  
Collaborative Study ◽  
Atomic Absorption Spectrophotometry ◽  
Absorption Method ◽  
Atomic Absorption Method ◽  
Absorption Spectrophotometry ◽  
Minor Nutrients

Abstract The atomic absorption method studied last year was re-examined and extended to include calcium and sodium. The procedures were submitted to 16 collaborators for determination of Ca, Cu, Fe, Mg, Mn, Na, and Zn. Results for Ca and Na were discouraging; further study is recommended.

Comparison of Methods for Determining Biuret in Urea

1979 ◽  
Vol 62 (1) ◽  
pp. 153-159
Author(s):  
Luis F Corominas ◽  
Victor M Boy ◽  
Manuel Guijosa
Keyword(s):  
Atomic Absorption ◽  
Spectrophotometric Determination ◽  
Atomic Absorption Spectrophotometry ◽  
International Organization ◽  
Comparison Of Methods ◽  
Definite Advantage ◽  
Absorption Spectrophotometry ◽  
Alternative Procedures ◽  
Aoac Method

Abstract The official first action AOAC method for the spectrophotometric determination of biuret in urea, 2.072—2.074, was compared with official first action AOAC method 2.C01-2.C03 (atomic absorption spectrophotometry), 2 simplified versions of 2.072-2.074, and modified versions of 2 alternative procedures of the International Organization for Standardization. Three synthetic urea samples (0.3, 1.4, and 3.0% biuret) and 1 commercial urea sample (1.0% biuret) were analyzed. The methods proved to be equivalent and none showed a definite advantage over 2.072-2.074. The purification of biuret and the interference by ammonia are also discussed.

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