Pyruvate as Substrate in the Determination of Serum Lactate Dehydrogenase Isoenzyme Activity

1974 ◽  
Vol 20 (11) ◽  
pp. 1462-1465 ◽  
Author(s):  
Doris McKenzie ◽  
A R Henderson
Keyword(s):  
Lactate Dehydrogenase ◽  
Serum Lactate ◽  
Serum Lactate Dehydrogenase ◽  
Dehydrogenase Isoenzyme

Abstract Lactate dehydrogenase isoenzyme activity is usually assessed, after the isoenzymes have been separated, by reactions involving lactate as the substrate. We describe a method for their assessment with pyruvate as the substrate. Precision is adequate as compared to the conventional methodology. Application of this type of approach (measurement of a decrease in fluorescence) to determination of other serum isoenzymes is briefly described.

6.1.1.3 Analytical Bias by Contamination from Hemolysis in Determination of Serum Lactate Dehydrogenase Isoenzyme 1 in Patients with Testis Germ Cell Tumors

1993 ◽  
Vol 98 (3) ◽  
pp. 293-298 ◽  
Author(s):  
Finn Edler von Eyben ◽  
Per Hyltoft Petersen ◽  
Ole Blaabjerg ◽  
Ebbe Lindegaard Madsen ◽  
Bent Nørgaard-Pedersen ◽  
...  
Keyword(s):  
Lactate Dehydrogenase ◽  
Germ Cell ◽  
Germ Cell Tumors ◽  
Serum Lactate ◽  
Serum Lactate Dehydrogenase ◽  
Dehydrogenase Isoenzyme

Serum Lactate Dehydrogenase Isoenzyme 1 and Relapse in Patients with Nonseminomatous Testicular Germ Cell Tumors Clinical Stage I

2001 ◽  
Vol 40 (4) ◽  
pp. 536-540 ◽  
Author(s):  
Finn Edler von Eyben ◽  
Ebbe Lindegaard Madsen ◽  
Ole Blaabjerg ◽  
Per Hyltoft Petersen ◽  
Hans von der Maase ◽  
...  
Keyword(s):  
Lactate Dehydrogenase ◽  
Germ Cell ◽  
Clinical Stage ◽  
Germ Cell Tumors ◽  
Serum Lactate ◽  
Stage I ◽  
Serum Lactate Dehydrogenase ◽  
Testicular Germ Cell Tumors ◽  
Testicular Germ Cell ◽  
Dehydrogenase Isoenzyme

A Rapid Electrophoretic Method for the Determination of the Isoenzymes of Serum Lactate Dehydrogenase

1966 ◽  
Vol 12 (5) ◽  
pp. 308-313 ◽  
Author(s):  
Albert W Opher ◽  
Charles S Collier ◽  
Joseph M Miller
Keyword(s):  
Enzyme Activity ◽  
Lactate Dehydrogenase ◽  
Quantitative Determination ◽  
Serum Lactate ◽  
Serum Lactate Dehydrogenase ◽  
Electrophoretic Method ◽  
The Individual ◽  
Electrophoretic Procedure ◽  
Purple Formazan

Abstract A convenient electrophoretic procedure for the separation and quantitation of lactate dehydrogenase (LDH) isoenzymes is described. The system uses polyacetate Sepraphore III strips.* The areas of activity are shown by incubation with an LDH substrate combined with tetra-nitro-blue-tetrazolium. The reduction of the latter to the purple formazan is quantitatively related to the enzyme activity. Quantitative determination of the individual colored areas is performed by densitometry.

Lactate dehydrogenase isoenzyme-1 in serum for detection of peri-operative myocardial infarction after cardiac surgery.

1988 ◽  
Vol 34 (12) ◽  
pp. 2469-2474 ◽  
Author(s):  
Z Rotenberg ◽  
J E Squires ◽  
M T Johnston ◽  
J Hoyt ◽  
R S Gibson ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Cardiac Surgery ◽  
Lactate Dehydrogenase ◽  
Valve Replacement ◽  
Artery Bypass ◽  
Reference Intervals ◽  
Serum Lactate ◽  
Serum Lactate Dehydrogenase ◽  
Present Measurement ◽  
Dehydrogenase Isoenzyme

Abstract We prospectively studied changes in serum lactate dehydrogenase isoenzyme-1 (LD-1, EC 1.1.1.27) in 99 consecutive patients after either coronary artery bypass grafting (CABG, n = 61), isolated cardiac-valve replacement (n = 24), or the two procedures combined (n = 14); 86 of these had no clinical evidence of peri-operative myocardial infarction (MI). Blood was sampled immediately after surgery and at 6-h intervals for up to 42 h thereafter. LD-1 was isolated by using the LD M-subunit antiserum. Samples from the non-MI patients were used to establish the reference intervals for LD-1. By 24 h after surgery, mean serum LD-1 values were higher (P less than 0.001) in non-MI patients who underwent isolated valve replacement (222 +/- 74 U/L) or combined CABG and valve replacement (266 +/- 58 U/L) than in 50 non-MI patients who underwent CABG alone (134 +/- 42 U/L). Separate reference intervals were determined for CABG and other patients at each sampling time. By 24 h after operation, LD-1 exceeded these reference intervals in the 10 CABG and two combined-procedure patients in whom other evidence of MI was present. Measurement of LD-1 24 to 42 h after cardiac surgery appears to be a useful test for the diagnosis of perioperative MI.

The Automated Determination of NAD-Coupled Enzymes

1966 ◽  
Vol 12 (5) ◽  
pp. 274-281 ◽  
Author(s):  
Stanley Morgenstern ◽  
Richard Flor ◽  
Gerald Kessler, ◽  
Bernard Klein
Keyword(s):  
Lactate Dehydrogenase ◽  
Excellent Agreement ◽  
Nicotinamide Adenine Dinucleotide ◽  
Colorimetric Method ◽  
Serum Lactate ◽  
Serum Lactate Dehydrogenase ◽  
Coupled Enzymes ◽  
Reduced Nicotinamide Adenine Dinucleotide ◽  
Automated Determination

Abstract A precise automated procedure developed for determination of serum lactate dehydrogenase, using the Robot Chemist, measures absorbance of a cuprous-neo-cuproine complex formed by coupled reduction of the cupric-neocuproine reagent with enzymatically generated reduced nicotinamide adenine dinucleotide. Activity values obtained by this method, by the identical automated colorimetric method on the AutoAnalyzer, and by an automated spectrophotometric (34O-mµ) procedure show excellent agreement.

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