An autosomal genetic linkage map of the sheep genome.

Abstract We report the first extensive ovine genetic linkage map covering 2070 cM of the sheep genome. The map was generated from the linkage analysis of 246 polymorphic markers, in nine three-generation full-sib pedigrees, which make up the AgResearch International Mapping Flock. We have exploited many markers from cattle so that valuable comparisons between these two ruminant linkage maps can be made. The markers, used in the segregation analyses, comprised 86 anonymous microsatellite markers derived from the sheep genome, 126 anonymous microsatellites from cattle, one from deer, and 33 polymorphic markers of various types associated with known genes. The maximum number of informative meioses within the mapping flock was 222. The average number of informative meioses per marker was 140 (range 18-209). Linkage groups have been assigned to all 26 sheep autosomes.

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Construction of a microsatellite-based genetic linkage map for half-smooth tongue sole Cynoglossus semilaevis

Current Zoology ◽

10.1093/czoolo/59.1.99 ◽

2013 ◽

Vol 59 (1) ◽

pp. 99-108 ◽

Cited By ~ 5

Author(s):

Wentao Song ◽

Guidong Miao ◽

Yongwei Zhao ◽

Yuze Niu ◽

Renyi Pang ◽

...

Keyword(s):

Linkage Map ◽

Genetic Linkage ◽

Genetic Linkage Map ◽

Cynoglossus Semilaevis ◽

Scar Markers ◽

Linkage Maps ◽

Linkage Groups ◽

Tongue Sole ◽

Genetic Linkage Maps ◽

Half Smooth Tongue Sole

Abstract The half-smooth tongue sole Cynoglossus semilaevis is an important cultured marine fish and a promising model fish for the study of sex determination. Sex-specific genetic linkage maps of half-smooth tongue sole were developed with 567 markers (565 microsatellite markers and two SCAR markers). The parents and F1 progeny (92 individuals) were used as segregating populations. The female map was composed of 480 markers in 21 linkage groups, covering a total of 1388.1 cM, with an average interval 3.06 cM between markers. The male map consisted of 417 markers in 21 linkage groups, spanning 1480.9 cM, with an average interval of 3.75 cM. The female and male maps had 474 and 416 unique positions, respectively. The genome length of half-smooth tongue sole was estimated to be 1522.9 cM for females and 1649.1cM for males. Based on estimations of map length, the female and male maps covered 91.1% and 89.8% of the genome, respectively. Furthermore, two female-specific SCAR markers, f-382 and f-783, were mapped on LG15f (linkage group 15 in female maps). The present study presents a mid-density genetic linkage map for half-smooth tongue sole. These improved genetic linkage maps may facilitate systematic genome searches to identify quantitative trait loci (QTL), such as disease resistance, growth and sex-related traits, and are very useful for marker-assisted selection breeding programs for economically important traits in half-smooth tongue sole.

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A Genetic Linkage Map of the Male Goat Genome

Genetics ◽

10.1093/genetics/144.1.279 ◽

1996 ◽

Vol 144 (1) ◽

pp. 279-305 ◽

Cited By ~ 1

Author(s):

Daniel Vaiman ◽

Laurent Schibler ◽

Florence Bourgeois ◽

Anne Oustry ◽

Yves Amigues ◽

...

Keyword(s):

In Situ Hybridization ◽

Linkage Map ◽

Genetic Linkage ◽

Genetic Linkage Map ◽

Polymorphic Markers ◽

Linkage Groups ◽

Coding Sequences ◽

Flanking Sequences ◽

Pcr Conditions

Abstract This paper presents a first genetic linkage map of the goat genome. Primers derived from the flanking sequences of 612 bovine, ovine and goat microsatellite markers were gathered and tested for amplification with goat DNA under standardized PCR conditions. This screen made it possible to choose a set of 55 polymorphic markers that can be used in the three species and to define a panel of 223 microsatellites suitable for the goat. Twelve half-sib paternal goat families were then used to build a linkage map of the goat genome. The linkage analysis made it possible to construct a meiotic map covering 2300 cM, i.e., >SO% of the total estimated length of the goat genome. Moreover, eight cosmids containing microsatellites were mapped by fluorescence in situ hybridization in goat and sheep. Together with 11 microsatellite-containing cosmids previously mapped in cattle (and supposing conservation of the banding pattern between this species and the goat) and data from the sheep map, these results made the orientation of 15 linkage groups possible. Furthermore, 12 coding sequences were mapped either genetically or physically, providing useful data for comparative mapping.

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Sheep linkage mapping: nineteen linkage groups derived from the analysis of paternal half-sib families.

Genetics ◽

10.1093/genetics/137.2.573 ◽

1994 ◽

Vol 137 (2) ◽

pp. 573-579

Author(s):

A M Crawford ◽

G W Montgomery ◽

C A Pierson ◽

T Brown ◽

K G Dodds ◽

...

Keyword(s):

Linkage Map ◽

Linkage Mapping ◽

Genetic Linkage ◽

Genetic Linkage Map ◽

Linkage Groups ◽

Sheep Genome ◽

Sheep Chromosome ◽

Sib Families

Abstract Nineteen linkage groups containing a total of 52 markers have been identified in the sheep genome after typing large paternal half-sib families. The linkage groups range in size from 2 markers showing no recombination to a group containing 6 markers covering approximately 30 cM of the sheep genome. Thirteen of the groups have been assigned to a sheep chromosome. Three groups contain markers from bovine syntenic groups U2, U7 and U29, and one other group contains a marker that has been mapped only in humans. The remaining three groups are unassigned. This information will provide a useful foundation for a genetic linkage map of sheep.

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DNA Markers from Different Linkage Regions of Watermelon Genome Useful in Differentiating among Closely Related Watermelon Genotypes

HortScience ◽

10.21273/hortsci.42.2.210 ◽

2007 ◽

Vol 42 (2) ◽

pp. 210-214 ◽

Cited By ~ 9

Author(s):

Amnon Levi ◽

Claude E. Thomas

Keyword(s):

Linkage Map ◽

Genetic Linkage ◽

Genetic Linkage Map ◽

Fragment Length Polymorphism ◽

Citrullus Lanatus ◽

Plant Introduction ◽

Polymorphic Markers ◽

Linkage Groups ◽

Breeding Lines ◽

Srap Markers

A genetic linkage map was previously constructed for watermelon using a wide testcross population [{Plant Accession Griffin 14113; Citrullus lanatus var. citroides (L.H. Baiely) Mansf.} × the watermelon cultivar New Hampshire Midget; NHM {(Citrullus lanatus (Thunb.) Matsum. & Nakai var. lanatus)} × United States Plant Introduction (PI) 386015 {Citrullus colocynthis (L.) Schrad.}]. One-hundred forty-six markers [randomly amplified polymorphic DNA (RAPD), intersimple sequence repeat (ISSR), amplified fragment length polymorphism (AFLP), and sequence-related amplified polymorphism (SRAP) markers] unique to NHM and representing different linkage groups on the map were tested for polymorphism among 24 watermelon cultivars limited in genetic diversity. Five (9.4%) of 53 RAPD, six (40.0%) of 15 ISSR, 30 (81.0%) of 37 AFLP, and 33 (80.5%) of 41 SRAP markers tested produced polymorphism among the 24 cultivars. The polymorphic markers used in this study are scattered throughout the watermelon genome. However, a large number (19 of the 30) of AFLP markers clustered on one linkage group on the map. The SRAP markers proved to be most effective in producing polymorphism and in representing different linkage regions of watermelon genome. The polymorphic markers represent all 10 large linkage groups and five of the nine small linkage groups (altogether 15 of 19 linkage groups) of the genetic linkage map constructed so far for watermelon. These polymorphic markers can be useful in DNA fingerprinting of cultivars, in testing seed purity of breeding lines, and in identifying triploid (seedless) hybrid watermelons derived from crosses between closely related tetraploid and diploid lines.

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A genetic linkage map of Pacific white shrimp (Litopenaeus vannamei): sex-linked microsatellite markers and high recombination rates

Genetica ◽

10.1007/s10709-006-9111-8 ◽

2006 ◽

Vol 131 (1) ◽

pp. 37-49 ◽

Cited By ~ 79

Author(s):

Liusuo Zhang ◽

Changjian Yang ◽

Yang Zhang ◽

Li Li ◽

Xiaoming Zhang ◽

...

Keyword(s):

Microsatellite Markers ◽

Linkage Map ◽

Litopenaeus Vannamei ◽

Genetic Linkage ◽

Genetic Linkage Map ◽

Pacific White Shrimp ◽

White Shrimp ◽

Recombination Rates

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A genetic linkage map of the sea cucumber,Apostichopus japonicus(Selenka), based on AFLP and microsatellite markers

Animal Genetics ◽

10.1111/j.1365-2052.2009.01900.x ◽

2009 ◽

Vol 40 (5) ◽

pp. 678-685 ◽

Cited By ~ 29

Author(s):

Q. Li ◽

L. Chen ◽

L. Kong

Keyword(s):

Microsatellite Markers ◽

Linkage Map ◽

Genetic Linkage ◽

Sea Cucumber ◽

Genetic Linkage Map ◽

Apostichopus Japonicus

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A second generation genetic linkage map for silver carp (Hypophthalmichehys molitrix) using microsatellite markers

Aquaculture ◽

10.1016/j.aquaculture.2013.06.027 ◽

2013 ◽

Vol 412-413 ◽

pp. 97-106 ◽

Cited By ~ 19

Author(s):

Wenjie Guo ◽

Jingou Tong ◽

Xiaomu Yu ◽

Chuankun Zhu ◽

Xiu Feng ◽

...

Keyword(s):

Microsatellite Markers ◽

Linkage Map ◽

Genetic Linkage ◽

Second Generation ◽

Genetic Linkage Map ◽

Silver Carp

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Two genetic linkage maps of mungbean using RFLP and RAPD markers

Australian Journal of Agricultural Research ◽

10.1071/ar99052 ◽

2000 ◽

Vol 51 (4) ◽

pp. 415 ◽

Cited By ~ 39

Author(s):

C. J. Lambrides ◽

R. J. Lawn ◽

I. D. Godwin ◽

J. Manners ◽

B. C. Imrie

Keyword(s):

Linkage Map ◽

Segregation Distortion ◽

Recombinant Inbred ◽

Genetic Linkage ◽

Rapd Markers ◽

Rflp Markers ◽

Linkage Maps ◽

Linkage Groups ◽

Genetic Linkage Maps ◽

Map Distance

Two genetic linkage maps of mungbean derived from the cross Berken ACC 41 are reported. The F2 map constructed from 67 individuals consisted of 110 markers (52 RFLP and 56 RAPD) that grouped into 12 linkage groups. The linked markers spanned a total map distance of 758.3 cM. A recombinant inbred (RI) population derived from the 67 F2 individuals was used for the generation of an additional linkage map. The RI map, composed entirely of RAPD markers, consisted of 115 markers in 12 linkage groups. The linked markers spanned a total map distance of 691.7 cM. Using a framework set of RFLP markers, the F2 map was compared with another F2 mungbean map constructed in Minnesota. In general, the order of these markers was consistent between maps. Segregation distortion was observed for some markers. 14.5% (16/110) of mapped F2 markers and 24% (28/115) of mapped RI markers segregated with distorted ratios. Segregation distortion occurred in each successive generation after the F2 . The regions of distortion identified in the Australian maps did not coincide with regions of the Minnesota map.

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