scholarly journals Impact of a human CMP-sialic acid transporter on recombinant glycoprotein sialylation in glycoengineered insect cells

Glycobiology ◽  
2012 ◽  
Vol 23 (2) ◽  
pp. 199-210 ◽  
Author(s):  
H. Mabashi-Asazuma ◽  
X. Shi ◽  
C. Geisler ◽  
C.-W. Kuo ◽  
K.-H. Khoo ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Insect Cells ◽  
Recombinant Glycoprotein ◽  
Acid Transporter

scholarly journals The Golgi CMP-sialic acid transporter: A new CHO mutant provides functional insights

Glycobiology ◽  
2008 ◽  
Vol 18 (11) ◽  
pp. 851-860 ◽  
Author(s):  
S. F. Lim ◽  
M. M. Lee ◽  
P. Zhang ◽  
Z. Song
Keyword(s):  
Sialic Acid ◽  
Acid Transporter

scholarly journals The Lysosomal Sialic Acid Transporter Sialin Is Required for Normal CNS Myelination

2009 ◽  
Vol 29 (49) ◽  
pp. 15355-15365 ◽  
Author(s):  
L. M. Prolo ◽  
H. Vogel ◽  
R. J. Reimer
Keyword(s):  
Sialic Acid ◽  
Acid Transporter ◽  
Cns Myelination

scholarly journals Transport of organic anions by the lysosomal sialic acid transporter: a functional approach towards the gene for sialic acid storage disease

FEBS Letters ◽  
1999 ◽  
Vol 446 (1) ◽  
pp. 65-68 ◽  
Author(s):  
Adrie C. Havelaar ◽  
Cecile E.M.T. Beerens ◽  
Grazia M.S. Mancini ◽  
Frans W. Verheijen
Keyword(s):  
Sialic Acid ◽  
Storage Disease ◽  
Organic Anions ◽  
Functional Approach ◽  
Acid Transporter ◽  
Sialic Acid Storage Disease

Targeting the expression of functional murine CMP-sialic acid transporter to the E. coli inner membrane

2007 ◽  
Vol 362 (3) ◽  
pp. 779-784 ◽  
Author(s):  
Andrea Maggioni ◽  
Mark von Itzstein ◽  
Rita Gerardy-Schahn ◽  
Joe Tiralongo
Keyword(s):  
Sialic Acid ◽  
Inner Membrane ◽  
E Coli ◽  
Acid Transporter

Method establishment for discerned immunogenicity assessment of a recombinant glycoprotein containing nonhuman sialic acid Neu5Gc residues

Bioanalysis ◽  
2017 ◽  
Vol 9 (18) ◽  
pp. 1385-1393 ◽  
Author(s):  
Marc-Olivier Montjovent ◽  
Linglong Zou ◽  
Heinrich Faust ◽  
Afsaneh Abdolzade-Bavil ◽  
Patrick M Liu ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Recombinant Glycoprotein ◽  
Immunogenicity Assessment

scholarly journals Structural basis for the delivery of activated sialic acid into Golgi for sialyation

2019 ◽  
Author(s):  
Emmanuel Nji ◽  
Ashutosh Gulati ◽  
Abdul Aziz Qureshi ◽  
Mathieu Coincon ◽  
David Drew
Keyword(s):  
Crystal Structure ◽  
Sialic Acid ◽  
Structural Basis ◽  
Golgi Membranes ◽  
Secretory Glycoproteins ◽  
Acid Transporter ◽  
Therapeutic Glycoproteins

AbstractThe decoration of secretory glycoproteins and glycolipids with sialic acid is critical to many physiological and pathological processes. Sialyation is dependent on a continuous supply of sialic acid into Golgi organelles in the form of CMP-sialic acid. Translocation of CMP-sialic acid into Golgi is carried out by the CMP-sialic acid transporter (CST). Mutations in human CST are linked to glycosylation disorders, and CST is important for glycopathway engineering, as it is critical for sialyation efficiency of therapeutic glycoproteins. The mechanism of how CMP-sialic acid is recognized and translocated across Golgi membranes in exchange for CMP is poorly understood. Here we have determined the crystal structure of a eukaryotic CMP-sialic acid transporter in complex with CMP. We conclude that the specificity of CST for CMP-sialic acid is established by the nucleotide CMP to such an extent, they are uniquely able to work both as passive and as (secondary) active antiporters.

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