Rapid determination of doxycycline based on luciferase assay of bacterial adenosine triphosphate

Total ATP (adenosine triphosphate) concentration is a useful biochemical parameter for detecting microbial biomass or biogeochemical activity anomalies in the natural environment. In this study, we describe the development and evaluation of a new version of in situ ATP analyzer improved for the continuous and quantitative determination of ATP in submarine environments. We integrated a transparent microfluidic device containing a microchannel for cell lysis and a channel for the bioluminescence L–L (luciferin–luciferase) assay with a miniature pumping unit and a photometry module for the measurement of the bioluminescence intensity. A heater and a temperature sensor were also included in the system to maintain an optimal temperature for the L–L reaction. In this study, the analyzer was evaluated in deep sea environments, reaching a depth of 200 m using a remotely operated underwater vehicle. We show that the ATP analyzer successfully operated in the deep-sea environment and accurately quantified total ATP within the concentration lower than 5 × 10−11 M.

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Determination of susceptibility ofStaphylococcus aureusto methicillin by luciferim-luciferase assay of bacterial adenosine triphosphate

Journal of Applied Bacteriology ◽

10.1111/j.1365-2672.1984.tb04706.x ◽

1984 ◽

Vol 56 (1) ◽

pp. 145-150 ◽

Cited By ~ 8

Author(s):

P.W. McWalter

Keyword(s):

Adenosine Triphosphate ◽

Luciferase Assay

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Adenosine Triphosphate Monitoring—A Rapid Determination of Microbial Contamination of Liquid Detergents

Proceedings of the 4th World Conference on Detergents ◽

10.1201/9781439822142.ch28 ◽

1999 ◽

Author(s):

Thomas Hilgers ◽

Helmut Kruessmann

Keyword(s):

Adenosine Triphosphate ◽

Microbial Contamination ◽

Rapid Determination

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MoS2 quantum dots as a unique fluorescent “turn-off–on” probe for the simple and rapid determination of adenosine triphosphate

Journal of Materials Chemistry B ◽

10.1039/c9tb00191c ◽

2019 ◽

Vol 7 (15) ◽

pp. 2549-2556 ◽

Cited By ~ 11

Author(s):

Yaping Zhong ◽

Tao Yi

Keyword(s):

Quantum Dots ◽

Adenosine Triphosphate ◽

Rapid Determination

MoS2 quantum dots were prepared as a fluorescent “turn-off–on” probe for the simple and rapid determination of adenosine triphosphate.

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Weak Beam Imaging and Diffraction Studies of Stacking Faults in Silicon

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100092918 ◽

1976 ◽

Vol 34 ◽

pp. 590-591

Author(s):

T. Y. Tan ◽

W. K. Tice

Keyword(s):

Fast Neutron ◽

Rapid Determination ◽

Stacking Faults ◽

Imaging Method ◽

Large Reduction ◽

Dislocation Loops ◽

Fringe Spacing ◽

Weak Beam ◽

Kinematical Theory

In studying ion implanted semiconductors and fast neutron irradiated metals, the need for characterizing small dislocation loops having diameters of a few hundred angstrom units usually arises. The weak beam imaging method is a powerful technique for analyzing these loops. Because of the large reduction in stacking fault (SF) fringe spacing at large sg, this method allows for a rapid determination of whether the loop is faulted, and, hence, whether it is a perfect or a Frank partial loop. This method was first used by Bicknell to image small faulted loops in boron implanted silicon. He explained the fringe spacing by kinematical theory, i.e., ≃l/(Sg) in the fault fringe in depth oscillation. The fault image contrast formation mechanism is, however, really more complicated.

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