The Regulatory Functions of the rolB and rolC Genes of Agrobacterium rhizogenes are Conserved in the Homologous Genes (Ngrol) of Nicotiana glauca in Tobacco Genetic Tumors

1995 ◽  
Vol 36 (6) ◽  
pp. 1003-1012 ◽  
Author(s):  
Noriko Nagata ◽  
Saori Kosono ◽  
Masami Sekine ◽  
Atsuhiko Shinmyo ◽  
Kunihiko Syono
Keyword(s):  
Agrobacterium Rhizogenes ◽  
Nicotiana Glauca ◽  
Homologous Genes ◽  
Regulatory Functions ◽  
Genetic Tumors ◽  
Rolb And Rolc Genes

scholarly journals Biological Activity of the Agrobacterium rhizogenes–Derived trolC Gene of Nicotiana tabacum and Its Functional Relation to Other plast Genes

2011 ◽  
Vol 24 (1) ◽  
pp. 44-53 ◽  
Author(s):  
Hanieh Mohajjel-Shoja ◽  
Bernadette Clément ◽  
Jonathan Perot ◽  
Malek Alioua ◽  
Léon Otten
Keyword(s):  
Agrobacterium Rhizogenes ◽  
Functional Relation ◽  
Starch Accumulation ◽  
Laccaria Bicolor ◽  
Sucrose Uptake ◽  
Nicotiana Glauca ◽  
Small Root ◽  
Leaf Chlorosis ◽  
Isolated Roots ◽  
Stimulation Of

Agrobacterium rhizogenes induces hairy roots through the activity of three essential T-DNA genes, rolA, rolB, and rolC, whereas the orf13 gene acts as an accessory root-inducing gene. rolB, rolC, and orf13 belong to the highly diverged plast gene family with remotely related representatives in the endomycorrhizal basidiomycete Laccaria bicolor. Nicotiana glauca and N. tabacum contain A. rhizogenes–derived T-DNAs with active plast genes. Here, we report on the properties of a rolC homolog in N. tabacum, trolC. Dexamethasone-inducible trolC and A4-rolC genes from A. rhizogenes A4 induce comparable, strong growth effects affecting all parts of the plants. Several have not been described earlier and were found to be very similar to the effects of the distantly related plast gene 6b. They include leaf chlorosis and starch accumulation, enations, increase of sucrose-dependent leaf disk expansion, growth of isolated roots on low-sucrose media, and stimulation of sucrose uptake by small root fragments. Collectively, our findings indicate that enhancement of sucrose uptake plays an important role in generating the complex 6b and rolC phenotypes and might be an ancestral property of the plast genes.

Evidence for the expression of the rol genes of Nicotiana glauca in genetic tumors of N. glauca x N. langsdorflli

1990 ◽  
Vol 220 (2) ◽  
pp. 177-180 ◽  
Author(s):  
Takanari Ichikawa ◽  
Yoshihiro Ozeki ◽  
Kunihiko Syöno
Keyword(s):  
Rol Genes ◽  
Nicotiana Glauca ◽  
Genetic Tumors
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