scholarly journals Immunohistochemical Analysis of LGR5 Expression in Pediatric Liver Disease

2015 ◽  
Vol 29 (S1) ◽  
Author(s):  
Zahida Khan ◽  
Anne Orr ◽  
George Michalopoulos ◽  
Sarangarajan Ranganathan
Keyword(s):  
Liver Disease ◽  
Immunohistochemical Analysis ◽  
Pediatric Liver ◽  
Pediatric Liver Disease ◽  
Lgr5 Expression

scholarly journals Pediatric Liver Disease in India

2021 ◽  
Author(s):  
Naresh Shanmugam ◽  
Malathy Sathyasekaran ◽  
Mohamed Rela
Keyword(s):  
Liver Disease ◽  
Pediatric Liver ◽  
Pediatric Liver Disease

The Kidney in Pediatric Liver Disease

2015 ◽  
Vol 17 (9) ◽  
Author(s):  
Robyn Greenfield Matloff ◽  
Ronen Arnon
Keyword(s):  
Liver Disease ◽  
Pediatric Liver ◽  
Pediatric Liver Disease

Iron and Pediatric Liver Disease

1994 ◽  
Vol 14 (03) ◽  
pp. 229-235 ◽  
Author(s):  
A. Knisely
Keyword(s):  
Liver Disease ◽  
Pediatric Liver ◽  
Pediatric Liver Disease

Pediatric Liver Disease

2018 ◽  
pp. 335-350
Author(s):  
Chatmanee Lertudomphonwanit ◽  
William F. Balistreri
Keyword(s):  
Liver Disease ◽  
Pediatric Liver ◽  
Pediatric Liver Disease

Nutritional Considerations in Pediatric Liver Disease

2014 ◽  
Vol 35 (11) ◽  
pp. 493-496
Author(s):  
Russell Cameron ◽  
Debora Kogan-Liberman
Keyword(s):  
Liver Disease ◽  
Pediatric Liver ◽  
Pediatric Liver Disease

scholarly journals Immunohistochemical Analysis of the Stem Cell Marker LGR5 in Pediatric Liver Disease

2017 ◽  
Vol 20 (1) ◽  
pp. 16-27 ◽  
Author(s):  
Zahida Khan ◽  
Anne Orr ◽  
George K Michalopoulos ◽  
Sarangarajan Ranganathan
Keyword(s):  
Stem Cell ◽  
Liver Disease ◽  
Liver Diseases ◽  
Chronic Liver ◽  
Stem Cell Marker ◽  
Cell Marker ◽  
Regenerative Response ◽  
Hepatocyte Regeneration ◽  
Pediatric Liver ◽  
Lgr5 Expression

Aims In regenerating liver, hepatic progenitor cells (HPCs) are recruited in response to injury; however, few highly specific human HPC markers exist for the hepatocyte lineage. Leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5), a Wnt-associated stem cell marker, has been extensively studied in intestinal stem cells, but little is known about its expression in human liver. We hypothesized that LGR5+ HPCs are induced in the regenerative response to pediatric liver injury. Methods and results Immunohistochemistry was used to characterize LGR5 expression in pediatric liver explants (n = 36). We found cytoplasmic LGR5 expression in all cases; although, much less was observed in acute hepatic necrosis compared to chronic liver diseases. In the latter cases, >50% of hepatocytes were LGR5+, signifying a robust regenerative response mainly in the periphery of regenerative nodules. Only weak LGR5 staining was noted in bile ducts, suggesting hepatocyte-specific expression at the interface. Conclusions Although we observed some degree of regenerative response in all cases, LGR5 was highly expressed in chronic liver disease, possibly due to alternate regeneration and reprogramming pathways. LGR5 is predominant in peri-septal hepatocytes rather than epithelial cell adhesion molecule (EpCAM) positive ductular reactions in chronic pediatric liver diseases and may represent a transitional HPC phenotype for the hepatocyte lineage. These studies are the first to support a unique role for LGR5 in human hepatocyte regeneration and as a potential predictive biomarker for recovery of liver function in children. Future work will also investigate the molecular mechanisms behind LGR5 expression.

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