IMMUNOHISTOCHEMICAL ANALYSIS OF THE STEM CELL MARKER LGR5 IN PEDIATRIC LIVER DISEASE

2016 ◽  
Author(s):  
Zahida Khan ◽  
Anne V. Orr ◽  
George K. Michalopoulos ◽  
Sarangarajan Ranganathan
Keyword(s):  
Stem Cell ◽  
Liver Disease ◽  
Immunohistochemical Analysis ◽  
Stem Cell Marker ◽  
Cell Marker ◽  
Pediatric Liver ◽  
Pediatric Liver Disease

scholarly journals Immunohistochemical Analysis of the Stem Cell Marker LGR5 in Pediatric Liver Disease

2017 ◽  
Vol 20 (1) ◽  
pp. 16-27 ◽  
Author(s):  
Zahida Khan ◽  
Anne Orr ◽  
George K Michalopoulos ◽  
Sarangarajan Ranganathan
Keyword(s):  
Stem Cell ◽  
Liver Disease ◽  
Liver Diseases ◽  
Chronic Liver ◽  
Stem Cell Marker ◽  
Cell Marker ◽  
Regenerative Response ◽  
Hepatocyte Regeneration ◽  
Pediatric Liver ◽  
Lgr5 Expression

Aims In regenerating liver, hepatic progenitor cells (HPCs) are recruited in response to injury; however, few highly specific human HPC markers exist for the hepatocyte lineage. Leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5), a Wnt-associated stem cell marker, has been extensively studied in intestinal stem cells, but little is known about its expression in human liver. We hypothesized that LGR5+ HPCs are induced in the regenerative response to pediatric liver injury. Methods and results Immunohistochemistry was used to characterize LGR5 expression in pediatric liver explants (n = 36). We found cytoplasmic LGR5 expression in all cases; although, much less was observed in acute hepatic necrosis compared to chronic liver diseases. In the latter cases, >50% of hepatocytes were LGR5+, signifying a robust regenerative response mainly in the periphery of regenerative nodules. Only weak LGR5 staining was noted in bile ducts, suggesting hepatocyte-specific expression at the interface. Conclusions Although we observed some degree of regenerative response in all cases, LGR5 was highly expressed in chronic liver disease, possibly due to alternate regeneration and reprogramming pathways. LGR5 is predominant in peri-septal hepatocytes rather than epithelial cell adhesion molecule (EpCAM) positive ductular reactions in chronic pediatric liver diseases and may represent a transitional HPC phenotype for the hepatocyte lineage. These studies are the first to support a unique role for LGR5 in human hepatocyte regeneration and as a potential predictive biomarker for recovery of liver function in children. Future work will also investigate the molecular mechanisms behind LGR5 expression.

scholarly journals CD133 and CD166 as stem cell markers for predicting the possibility of developing prostatic cancer in cases of benign prostatic hyperplasia

2019 ◽  
Vol 12 (3) ◽  
pp. 1403-1416
Author(s):  
Khalida I. Noel ◽  
Mustafa M. Ibraheem ◽  
Basim S. Ahmed ◽  
Ahmed F. Hameed ◽  
Nibras H. Khamees ◽  
...  
Keyword(s):  
Stem Cell ◽  
Benign Prostatic Hyperplasia ◽  
Immunohistochemical Analysis ◽  
Prostatic Hyperplasia ◽  
Stem Cell Marker ◽  
Stem Cell Markers ◽  
Cell Marker ◽  
Over Expression ◽  
Prostatic Epithelium ◽  
Prostatic Diseases

Benign and malignant prostatic diseases are generally well-known in the world. Accordingly, this research is planned to assess the immunohistochemical analysis of CD133 and CD166 in the prostatic epithelium in samples of benign prostatic hyperplasia (BPH) and normal looking epithelium around prostatic adenocarcinoma samples (PCa) and to explore the opportunity of malignant alterations in benign tissue. The prostate samples were divided into 2 groups; 50 BPH samples, and 50 normally looking tissue surrounding prostatic carcinoma samples (NPCA). The samples were treated for immunohistochemical examination of CD133 and CD166. Over expression of CD133 appeared in the BPH group which was statistically significant as compared to NPCA group. Conversely, over expression of CD166 stem cell marker in NPCA group than BPH group as it was significant statistically. CD166 is a stem cell marker for tissue tumorigenicity, while the positive expression of CD133 is not of value for cancer initiation.

scholarly journals Immunohistochemical Analysis of LGR5 Expression in Pediatric Liver Disease

2015 ◽  
Vol 29 (S1) ◽  
Author(s):  
Zahida Khan ◽  
Anne Orr ◽  
George Michalopoulos ◽  
Sarangarajan Ranganathan
Keyword(s):  
Liver Disease ◽  
Immunohistochemical Analysis ◽  
Pediatric Liver ◽  
Pediatric Liver Disease ◽  
Lgr5 Expression

The Corneal Epithelial Stem Cell Marker - Novel Combination of High Expression of p63 and a Large N/C Ratio

2008 ◽  
Vol 1 (3) ◽  
pp. 95-105
Author(s):  
Arpitha P. ◽  
Prajna N. V. ◽  
Srinivasan M. ◽  
Muthukkaruppan V. R.
Keyword(s):  
Stem Cell ◽  
High Expression ◽  
Stem Cell Marker ◽  
Cell Marker ◽  
Corneal Epithelial ◽  
Epithelial Stem Cell ◽  
Large N
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