The breakdown of ribonucleic acid in the cell nucleus

1963 ◽  
Vol 158 (970) ◽  
pp. 79-87 ◽  
Keyword(s):  
Hela Cell ◽  
Cell Nucleus ◽  
Ribonucleic Acid ◽  
Polynucleotide Phosphorylase

The enzyme responsible for the breakdown of the rapidly labelled ribonucleic acid ( RNA ) in the HeLa cell nucleus has the properties of a polynucleotide phosphorylase. This enzyme acts preferentially on the rapidly labelled RNA and appears to degrade it to nucleoside-5' diphosphates. Both the rapidly labelled RNA and the enzyme which degrades it are apparently attached to the chromosome.

The Reactivation of the Red Cell Nucleus

1967 ◽  
Vol 2 (1) ◽  
pp. 23-28 ◽  
Author(s):  
H. HARRIS
Keyword(s):  
Hela Cell ◽  
Cell Nucleus ◽  
Rna Synthesis ◽  
Direct Relationship ◽  
Nuclear Volume ◽  
Red Cell ◽  
Primary Event ◽  
Rna And Dna ◽  
Nuclear Enlargement ◽  
Made In

When the nucleus of a mature hen erythrocyte is introduced into the cytoplasm of a HeLa cell it resumes the synthesis of RNA and DNA. This reactivation of the red cell nucleus in the heterokaryon is associated with a marked increase in its volume. There is a direct relationship between the volume of the nucleus and the amount of RNA which it makes. The nuclear enlargement is not a consequence of increased RNA synthesis, or of DNA synthesis: enlargement is the primary event, and the increase in RNA synthesis is determined by it. The possibility is considered that changes in nuclear volume may regulate not only the amount of RNA made in the nucleus but also the areas of chromatin on which it is made.

Infectivity of ribonucleic acid of poliovirus on HeLa cell monolayers

Virology ◽  
1958 ◽  
Vol 5 (1) ◽  
pp. 172-173 ◽  
Author(s):  
Hattie E. Alexander ◽  
Gebhard Koch ◽  
Isabel Morgan Mountain ◽  
Katherine Sprunt ◽  
Olga Van Damme
Keyword(s):  
Hela Cell ◽  
Ribonucleic Acid ◽  
Cell Monolayers

scholarly journals Biosynthesis of a hypermodified nucleotide in Saccharomyces carlsbergensis 17S and HeLa-cell 18S ribosomal ribonucleic acid

1978 ◽  
Vol 169 (1) ◽  
pp. 71-77 ◽  
Author(s):  
R C Brand ◽  
J Klootwijk ◽  
R J Planta ◽  
B E H Maden
Keyword(s):  
Hela Cell ◽  
Hela Cells ◽  
Ribonucleic Acid ◽  
18S Rrna ◽  
Saccharomyces Carlsbergensis ◽  
Ribosomal Ribonucleic Acid ◽  
Precursor Molecules

The biosynthesis of a hypermodified nucleotide, similar to or identical with 3-(3-amino-3-carboxypropyl)-1-methylpseudouridine monophosphate, present in Saccharomyces carlsbergensis 17S and HeLa-cell 18S rRNA, was investigated with respect to the sequence of reactions required for synthesis and their timing in ribosome maturation. In both yeast and HeLa cells methylation precedes attachment of the 3-amino-3-carboxypropyl group. In yeast the methylated precursor nucleotide was tentatively characterized as 1-methylpseudouridine. This precursor nucleotide was demonstrated in both 37S and most of the cytoplasmic 18S pre-rRNA (rRNA precursor) molecules. The synthesis of the hypermodified nucleotide is completed just before the final cleavage of 18S pre-rRNA to give 17S rRNA, so that the final addition of the 3-amino-3-carboxypropyl group is a cytoplasmic event. Comparable experiments with HeLa cells indicated that formation of 1-methylpseudouridine occurs at the level of 45S RNA and addition of the 3-amino-3-carboxypropyl group occurs in the cytoplasm on newly synthesized 18S RNA.

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