scholarly journals Rufibacter roseus sp. nov., isolated from radiation-polluted soil

2015 ◽  
Vol 65 (Pt_5) ◽  
pp. 1572-1577 ◽  
Author(s):  
Zhi-Dong Zhang ◽  
Mei-Ying Gu ◽  
Jing Zhu ◽  
Shan-Hui Li ◽  
Li-Juan Zhang ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Strain ◽  
Gene Sequence ◽  
Polluted Soil ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Content Type ◽  
Link Type

A rose, Gram-stain-negative, aerobic, rod-shaped bacterium that was motile by gliding, and designated strain H359T, was isolated from radiation-polluted soil (with high Cs137) from the Xinjiang Uygur Autonomous Region of PR China and subjected to a polyphasic taxonomic analysis. The isolate grew optimally at 30 °C and pH 7.0. It grew with NaCl up to 4 % (w/v). 16S rRNA gene sequence analysis indicated that strain H359T belonged to the genus Rufibacter , a member of the family Cytophagaceae , with Rufibacter tibetensis CCTCC AB 208084T as its closest phylogenetic relative, having 96.1 % 16S rRNA gene sequence similarity to the type strain. Strain H359T contained menaquinone-7 (MK-7) as the predominant menaquinone, and the major fatty acids were iso-C15 : 0, summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B), summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C16 : 1ω5c. The polar lipid profile had phosphatidylethanolamine as the major component. The DNA G+C content was 43.9 mol%. Based on phenotypic, genotypic and phylogenetic evidence, strain H359T represents a novel species of the genus Rufibacter , for which the name Rufibacter roseus sp. nov. is proposed. The type strain is H359T ( = CPCC 100615T = KCTC 42217T).

Bowmanella yangjiangensis sp. nov. and Amphritea pacifica sp. nov., isolated from mariculture fishponds in China

2021 ◽  
Vol 71 (8) ◽  
Author(s):  
Zhipeng Cai ◽  
Huibin Lu ◽  
Youfeng Qian ◽  
Letian Chen ◽  
Meiying Xu
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Strain ◽  
Gene Sequence ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Content Type ◽  
Link Type ◽  
Sequence Similarities

Four Gram-stain-negative, catalase- and oxidase-positive, rod-shaped and motile strains (Y26, Y57T, ZJ14WT and RP18W) were isolated from mariculture fishponds in PR China. Comparisons based on 16S rRNA gene sequences showed that strains Y26 and Y57T share 16S rRNA gene sequence similarities in the range of 95.1−98.5 % with species of the genus Bowmanella , and strains ZJ14WT and RP18W share 16S rRNA gene sequence similarities in the range of 96.7 −98.8 % with species of the genus Amphritea , respectively. The genome sizes of strains Y26, Y57T, ZJ14WT and RP18W were about 4.85, 5.40, 4.70 and 4.70 Mbp with 49.5, 51.7, 51.2 and 51.3 mol% G+C content, respectively. The calculated pairwise OrthoANIu values among strains Y26, Y57T and species of the genus Bowmanella were in the range of 72.6−83.1 %, but the value between strains Y26 and Y57T was 96.2 %. The pairwise OrthoANIu values among strains ZJ14WT, RP18W and other species of the genus Amphritea were all less than 93.9 %, but the value between strains ZJ14WT and RP18W was 99.3 %. Q-8 was the major respiratory quinone of strains Y26, Y57T, ZJ14WT and RP18W, and the major fatty acids of these strains were all C16 : 1 ω7c, C16 : 0 and C18 : 1 ω7c. The predominant polar lipids of strains Y26 and Y57T included phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and phosphatidylinositol, but strains ZJ14WT and RP18W only contained phosphatidylethanolamine and phosphatidylglycerol. Combining phenotypic, biochemical and genotypic characteristics, strains Y26 and Y57T should belong to the same species and represent a novel member of the genus Bowmanella , and strains ZJ14WT and RP18W should belong to the same species and represent a novel member of the genus Amphritea , for which the names Bowmanella yangjiangensis sp. nov. (type strain Y57T=GDMCC 1.2180T=KCTC 82439T) and Amphritea pacifica sp. nov. (type strain ZJ14WT=GDMCC 1.2203T=KCTC 82438T) are proposed.

scholarly journals Aridibacter famidurans gen. nov., sp. nov. and Aridibacter kavangonensis sp. nov., two novel members of subdivision 4 of the Acidobacteria isolated from semiarid savannah soil

2014 ◽  
Vol 64 (Pt_6) ◽  
pp. 1866-1875 ◽  
Author(s):  
Katharina J. Huber ◽  
Pia K. Wüst ◽  
Manfred Rohde ◽  
Jörg Overmann ◽  
Bärbel U. Foesel
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Strain ◽  
Type Species ◽  
Gene Sequence ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Content Type ◽  
Link Type

Acidobacteria constitute an abundant fraction of the soil microbial community and are currently divided into 26 subdivisions. Most cultivated members of the Acidobacteria are affiliated with subdivision 1, while only a few representatives of subdivisions 3, 4, 8, 10 and 23 have been isolated and described so far. Two novel isolates of subdivision 4 of the Acidobacteria were isolated from subtropical savannah soils and are characterized in the present work. Cells of strains A22_HD_4HT and Ac_23_E3T were immotile rods that divided by binary fission. Colonies were pink and white, respectively. The novel strains A22_HD_4HT and Ac_23_E3T were aerobic mesophiles with a broad range of tolerance towards pH (4.0–9.5 and 3.5–10.0, respectively) and temperature (15–44 and 12–47 °C, respectively). Both showed chemo-organoheterotrophic growth on some sugars, the amino sugar N-acetylgalactosamine, a few amino acids, organic acids and various complex protein substrates. Major fatty acids of A22_HD_4HT and Ac_23_E3T were iso-C15 : 0, summed feature 1 (C13 : 0 3-OH/iso-C15 : 1 H), summed feature 3 (C16 : 1ω7c/C16 : 1ω6c) and anteiso-C17 : 0. The major quinone was MK-8; in addition, MK-7 occurred in small amounts. The DNA G+C contents of A22_HD_4HT and Ac_23_E3T were 53.2 and 52.6 mol%, respectively. The closest described relative was Blastocatella fastidiosa A2-16T, with 16S rRNA gene sequence identity of 93.2 and 93.3 %, respectively. Strains A22_HD_4HT and Ac_23_E3T displayed 16S rRNA gene sequence similarity of 97.4 % to each other. On the basis of the low DNA–DNA hybridization value, the two isolates represent different species. Based on morphological, physiological and molecular characteristics, the new genus Aridibacter gen. nov. is proposed, with two novel species, the type species Aridibacter famidurans sp. nov. (type strain A22_HD_4HT = DSM 26555T = LMG 27985T) and a second species, Aridibacter kavangonensis sp. nov. (type strain Ac_23_E3T = DSM 26558T = LMG 27597T).

scholarly journals Description of Sphingorhabdus planktonica gen. nov., sp. nov. and reclassification of three related members of the genus Sphingopyxis in the genus Sphingorhabdus gen. nov.

2013 ◽  
Vol 63 (Pt_4) ◽  
pp. 1342-1349 ◽  
Author(s):  
Mareike Jogler ◽  
Hong Chen ◽  
Julia Simon ◽  
Manfred Rohde ◽  
Hans-Jürgen Busse ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Strain ◽  
Type Species ◽  
Gene Sequence ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Content Type ◽  
Link Type

A previously undescribed aerobic, non-sporulating bacterium, strain G1A_585T, was isolated from an oligotrophic freshwater lake in Bavaria, Germany. The rod-shaped cells were Gram-stain-negative and non-motile. Based on 16S rRNA gene sequence similarity, strain G1A_585T was a member of the family Sphingomonadaceae and shared <95.2 % similarity with type strains of all members of the most closely related genus, Sphingopyxis . Phyogenetically, the isolate shared a root with strains of three marine species, Sphingopyxis flavimaris DSM 16223T, Sphingopyxis marina DSM 22363T and Sphingopyxis litoris DSM 22379T. The polar lipids of strain G1A_585T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidyldimethylethanolamine, phosphatidylcholine, sphingoglycolipids, three glycolipids and one unknown lipid. Ubiquinone-10 was the dominant quinone (93.1 %) and ubiquinone-9 (6.5 %) was also detected. The major cellular fatty acids were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c; 38.2 %); C16 : 1ω7c (33.6 %) and C14 : 0 2-OH (17.8 %). The major polyamine was spermidine and traces of 1,3-diaminopropane, putrescine and spermine were also detected. The DNA G+C content of strain G1A_585T was 55.7 mol% and the isolate was oxidase- and catalase-positive. Based on the phylogenetic relationship, the low DNA G+C content compared with most other members of the genus Sphingopyxis and the presence of signature nucleotides in the 16S rRNA gene sequence, a novel species in a new genus and species, Sphingorhabdus planktonica gen. nov., sp. nov., is proposed; the type strain of Sphingorhabdus planktonica is G1A_585T ( = DSM 25081T  = LMG 26646T). Because Sphingopyxis flavimaris DSM 16223T, Sphingopyxis marina DSM 22363T and Sphingopyxis litoris DSM 22379T form a phylogenetic group together with strain G1A_585T that is clearly separated from all other known Sphingopyxis strains and share signature nucleotides, these three Sphingopyxis strains are reclassified as members of the proposed novel genus Sphingorhabdus: Sphingorhabdus flavimaris comb. nov. (type strain SW-151T = DSM 16223T = KCTC 12232T), Sphingorhabdus marina comb. nov. (type strain FR1087T = DSM 22363T = IMSNU 14132T = KCTC 12763T = JCM 14161T) and Sphingorhabdus litoris comb. nov. (type strain FR1093T = DSM 22379T = IMSNU 14133T = KCTC 12764T = JCM 14162T).

Sphingomicrobium lutaoense gen. nov., sp. nov., isolated from a coastal hot spring

2012 ◽  
Vol 62 (Pt_6) ◽  
pp. 1326-1330 ◽  
Author(s):  
Peter Kämpfer ◽  
A. B. Arun ◽  
Chiu-Chung Young ◽  
Hans-Jürgen Busse ◽  
Johannes Kassmannhuber ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Strain ◽  
Gene Sequence ◽  
Hot Spring ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Content Type ◽  
Link Type

A yellowish pigmented, Gram-negative, rod-shaped, non-spore-forming bacterium (strain CC-TBT-3T), was isolated on marine agar 2216 from a coastal hot spring of Green Island (Lutao), located off Taituang, Taiwan. 16S rRNA gene sequence analysis of strain CC-TBT-3T showed a relatively low similarity (<95.5 %) to representatives of the genera Novosphingobium , Sphingosinicella and Sphingomonas of the Sphingomonadaceae , with the most related strain being the type strain of Novosphingobium soli . In addition to the relatively low 16S rRNA gene sequence similarity to members of established species, the isolate also showed some unique chemotaxonomic features, including the presence of some glycolipids with unusual chromatographic behaviour. The major components of the polar lipid profile were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, sphingoglycolipid and three unidentified glycolipids. The major respiratory quinone was ubiquinone Q-10. The polyamine pattern was characterized by the triamine sym-homospermidine as a major component. Although the predominant fatty acids were C18 : 1ω7c and summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH), the isolate did not show the typical hydroxyl fatty acids, such as C14 : 0 2-OH, C15 : 0 2-OH and C16 : 0 2-OH, found in members of the genera Novosphingobium , Sphingomonas and Sphingosinicella , but showed instead high amounts of C18 : 1 2-OH (12.0 %). The DNA G+C content of strain CC-TBT-3T was 63.4 mol%. 16S rRNA gene sequence, chemotaxonomic and physiological analyses revealed that strain CC-TBT-3T represents a novel species in a new genus in the family Sphingomonadaceae for which the name Sphingomicrobium lutaoense gen. nov., sp. nov. is proposed; the type strain is of the type species S. lutoaense, CC-TBT-3T ( = DSM 24194T = CCM 7794T).

scholarly journals Martelella radicis sp. nov. and Martelella mangrovi sp. nov., isolated from mangrove sediment

2014 ◽  
Vol 64 (Pt_9) ◽  
pp. 3104-3108 ◽  
Author(s):  
De-Chao Zhang ◽  
Rosa Margesin
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Strain ◽  
Type Species ◽  
Gene Sequence ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Content Type ◽  
Link Type

Two Gram-stain-negative, non-motile, rod-shaped bacterial strains, designated BM5-7T and BM9-1T were isolated from soil of the root system of a mangrove forest. Phylogenetic analysis based on 16S rRNA gene sequences showed that the two isolates belong to the genus Martelella . The chemotaxonomic characteristics of these isolates included the presence of C19 : 0 cyclo ω8c and C18 : 1ω7c as the major cellular fatty acids and Q-10 as the dominant ubiquinone. The genomic DNA G+C contents of strains BM5-7T and BM9-1T were 61.0 and 59.7 mol% (HPLC method), respectively. The 16S rRNA gene sequence similarity between the two strains was 98.1 %, but DNA–DNA hybridization indicated 44 % relatedness. Strains BM5-7T and BM9-1T exhibited 16S rRNA gene sequence similarities of 98.0–99.2 % and 97.7–98.1 %, respectively, with type strains of Martelella endophytica and Martelella mediterranea . Combined data from phenotypic, phylogenetic and DNA–DNA relatedness studies demonstrated that strains BM5-7T and BM9-1T are representatives of two novel species of the genus Martelella , for which the names Martelella radicis sp. nov. (type strain BM5-7T = DSM 28101T = LMG 27958T) and Martelella mangrovi sp. nov. (type strain BM9-1T = DSM 28102T = LMG 27959T) are proposed.

Geodermatophilus soli sp. nov. and Geodermatophilus terrae sp. nov., two actinobacteria isolated from grass soil

2013 ◽  
Vol 63 (Pt_7) ◽  
pp. 2625-2629 ◽  
Author(s):  
Long Jin ◽  
Hyung-Gwan Lee ◽  
Hee-Sik Kim ◽  
Chi-Yong Ahn ◽  
Hee-Mock Oh
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Strain ◽  
Gene Sequence ◽  
Diaminopimelic Acid ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Content Type ◽  
Link Type

Two strains, PB34T and PB261T, were isolated from grass soil sampled in Daejeon, Republic of Korea. Comparative 16S rRNA gene sequence studies showed the two bacteria to be clearly affiliated with the phylum Actinobacteria and most closely related to the genus Geodermatophilus , showing 16S rRNA gene sequence similarities to the type strains of species of the genus Geodermatophilus of 95.0–96.3 % and sharing 98.5 % similarity between the two strains. The two strains were Gram-stain-positive, aerobic, motile and rod-shaped bacteria. The peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid. The predominant menaquinones were MK-9(H4) and MK-9(H0). The major fatty acids were iso-C15 : 0, iso-C16 : 0, iso-C17 : 0 and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) for strain PB34T and iso-C14 : 0, iso-C15 : 0, iso-C16 : 0 and C16 : 0 for strain PB261T. The G+C contents of the genomic DNA of strains PB34T and PB261T were 73.2 mol% and 74.1 mol%, respectively. Thus, based on the evidence of a polyphasic study, it is proposed that strains PB34T and PB261T represent two novel species, for which the names Geodermatophilus soli sp. nov. (type strain PB34T = KCTC 19880T = JCM 17785T) and Geodermatophilus terrae sp. nov. (type strain PB261T = KCTC 19881T = JCM 17786T) are proposed.

scholarly journals Microbacterium kyungheense sp. nov. and Microbacterium jejuense sp. nov., isolated from salty soil

2014 ◽  
Vol 64 (Pt_7) ◽  
pp. 2267-2273 ◽  
Author(s):  
MooChang Kook ◽  
Heung-Min Son ◽  
Tae-Hoo Yi
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Strain ◽  
Type Species ◽  
Gene Sequence ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Content Type ◽  
Link Type

Two novel strains, THG-C26T and THG-C31T, were characterized using a polyphasic approach to determine their taxonomic positions. These two isolates were aerobic, Gram-stain-positive, non-motile, non-spore-forming and rod-shaped. 16S rRNA gene sequences and phenotypic features including chemotaxonomic characteristics indicated that the two isolates clearly represented members of the genus Microbacterium . The quinone systems of strains THG-C26T and THG-C31T contained MK-12/MK-13 as major menaquinones. The diamino acid in cell-wall hydrolysates of the two strains was ornithine. The major fatty acids were iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0. The polyamine pattern had spermidine as the predominant component. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol and unidentified glycolipids. Phenotypic characteristics supported the affiliation of strains THG-C26T and THG-C31T to the genus Microbacterium . Chemotaxonomic data and DNA–DNA relatedness values allowed differentiation of these strains from other species of the genus Microbacterium with validly published names. Strains THG-C26T and THG-C31T showed highest 16S rRNA gene sequence similarities with Microbacterium resistens DMMZ 1710T (98.5 %) and Microbacterium trichothecenolyticum IFO 15077T (98.8 %), respectively, and the 16S rRNA gene sequence similarity between them was 99.0 %. DNA–DNA hybridization values between the novel isolates and strains of other species of the genus Microbacterium with validly published names were 4–25 %. Therefore, strains THG-C26T and THG-C31T are considered to represent two novel species of the genus Microbacterium , for which the names Microbacterium kyungheense sp. nov. [type strain THG-C26T ( = KACC 17124T = JCM 18735T)] and Microbacterium jejuense sp. nov. [type strain THG-C31T ( = KACC 17123T = JCM 18734T)] are proposed.

scholarly journals Aidingimonas lacisalsi sp. nov., isolated from a salt lake in China

2020 ◽  
Vol 70 (3) ◽  
pp. 1678-1683 ◽  
Author(s):  
Tong-Wei Guan ◽  
Li-Sha Tian ◽  
Shu-Kun Tang ◽  
Lei Tian ◽  
Yu-Zhou Feng
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Strain ◽  
Gene Sequence ◽  
Salt Lake ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Content Type ◽  
Link Type

A novel bacterium, XHU 5135T, belonging to the genus Aidingimonas , was isolated from a salt lake sample collected in Xinjiang Province, north-west PR China. The isolate was Gram-stain-negative, rod-shaped and non-motile. The strain was catalase-positive and oxidase-negative. Growth occurred at NaCl concentrations of 5–25 % (optimum, 10–13 %), at 13–41 °C (35–37 °C) and at pH 6.0–10.0 (pH 7.0–8.0). The predominant ubiquinone was Q-9. The major fatty acids were C19 : 0 cyclo ω8c and C16 : 0. The G+C content of the genomic DNA was 58.1 mol%. The affiliation of strain XHU 5135T with the genus Aidingimonas was confirmed by 16S rRNA gene sequence comparisons. The closest type strain was Aidingimonas halophile YIM 90637T, which showed a 16S rRNA gene sequence similarity of 97.5 %. The ANI value between XHU 5135T and the closest type strain was 80.01 %. The estimated digital DNA–DNA hybridization estimate value between strain XHU 5135T and the closest type strain was 22.80 %. Phenotypically, the characteristics of XHU 5135T were shown to differ from the most closely related species, A. halophila . On the basis of the data from this polyphasic study, strain XHU 5135T represents a novel species of the genus Aidingimonas , for which the name Aidingimonas lacisalsi sp. nov. is proposed. The type strain is strain XHU 5135T (=CCTCC AB 2016344T=KCTC 42945T=DSM 104700T).

scholarly journals Microbacterium populi sp. nov., isolated from Populus×euramericana bark

2015 ◽  
Vol 65 (Pt_5) ◽  
pp. 1444-1449 ◽  
Author(s):  
Yong Li ◽  
Tao Wang ◽  
Wei Fang ◽  
Han Xue ◽  
Chun-gen Piao ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Strain ◽  
Type Species ◽  
Gene Sequence ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Content Type ◽  
Link Type

Five non-spore-forming, aerobic and Gram-stain-positive bacterial strains, 10-107-8T, 1C-4, NHI3_6, 4107_1_2, and 3D-3, were isolated from Populus×euramericana bark collected in Puyang City, Henan Province, PR China. The isolates grew at 15–40 °C and pH 5–10. The optimum temperature and pH for growth were 30 °C and pH 8.0, respectively. Chemotaxonomic features included MK-10 and MK-11 as major menaquinones (type strain); predominating iso- and anteiso-branched cellular fatty acids; diphosphatidylglycerol and phosphatidylglycerol as major polar lipids (type strain); ornithine as the principal diamino acid of the cell-wall peptidoglycan (type strain); glycolyl type as cell-wall acyl type; and DNA G+C content of 66.8–67.6 mol%. These features were consistent with classification in the genus Microbacterium . Analysis of 16S rRNA gene sequence data indicated that the five isolates belonged to the genus Microbacterium and were closely related to Microbacterium halotolerans . A high 16S rRNA gene sequence similarity of 96.97 % to M. halotolerans YIM 70130T was observed. The five isolates showed less than 96.20 % 16S rRNA gene sequence similarity to the other species of the genus Microbacterium with validly published names. DNA–DNA relatedness of the five isolates with M. halotolerans JCM 13013T ranged from 35.62 % to 44.36 %. Considering the results of 16S rRNA gene sequence analysis and the physiological and biochemical characteristics, we propose that the five strains should be assigned to a novel species of the genus Microbacterium . The name proposed for the five strains is Microbacterium populi sp. nov., and the type strain is 10-107-8T ( = CFCC 11275T = KCTC 29152T).

Classification of Bisgaard’s taxa 14 and 32 and a taxon from kestrels demonstrating satellitic growth and proposal of Spirabiliibacterium gen. nov., including the description of three species: Spirabiliibacterium mucosae sp. nov., Spirabiliibacterium pneumoniae sp. nov. and Spirabiliibacterium falconis sp. nov.

2019 ◽  
Vol 71 (3) ◽  
Author(s):  
Magne Bisgaard ◽  
Henrik Christensen
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Strain ◽  
Gene Sequence ◽  
Sequence Similarity ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Content Type ◽  
Link Type

Avian Pasteurella -like organisms tentatively named taxon 14 of Bisgaard have been obtained from different lesions in birds including ducks, turkeys, pigeons, geese and peafowl. Taxon 32 of Bisgaard was first reported from lesions in pigeon hawks (Accipiter gentiles). The taxon isolated from kestrels (Falco tinnunculus) was V-factor dependent and originally reported as Haemophilus-like. The results of 16S rRNA gene sequence based phylogenetic analysis recently indicated that the taxa 14 and 32 and the kestrel taxon were located in a monophyletic group distantly related to [ Pasteurella ] testudinis with 92–93 % 16S rRNA gene sequence similarity. Comparison of 41 conserved protein sequences confirmed the monophyletic nature of the three taxa. Partial rpoB gene sequencing of 43 strains of taxon 14, taxon 32 and the kestrel taxon showed a relationship between taxon 14 and 32 of 88.2–90.0 % similarity. Within taxon 14, 93.3–100 % similarity was found, whereas the two strains of taxon 32 showed 99.8 % rpoB similarity. Sequencing of 16S rRNA genes of strains representing the rpoB diversity outlined showed more than 98 % similarity within taxon 14 and 99.4 % within taxon 32, while the kestrel strains showed 100 % 16S rRNA gene sequence similarity. A new genus, Spirabiliibacterium gen. nov., is proposed to include taxon 14, taxon 32 and the kestrel taxon. Phenotypically, members of the genus Spirabiliibacterium can be separated from members of the genera Aggregatibacter , Avibacterium and Volucribacter by maltose, oxidase and methyl red, respectively. Two or more phenotypic characters separate members of the genus Spirabiliibacterium from members of the remaining 27 genera of the family Pasteurellaceae .The G+C content of DNA ranged from 42.9 to 51.2 % (genome sequence) for members of the genus Spirabiliibacterium. The type strain of Spirabiliibacterium mucosae (taxon 14 of Bisgaard) is 20609/3T (=CCUG 16499T=DSM 111429T=HIM 913-3T). The type strain of Spirabiliibacterium pneumoniae is HPA106T (=CCUG 74731T=DSM 111430T). The type strain of Spirabiliibacterium falconis (kestrel taxon) is IPDH 2176T (=NCTC 11878T=CCUG 28587T).

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