Candida borneonana sp. nov., a methanol-assimilating anamorphic yeast isolated from decaying fruit

2012 ◽  
Vol 62 (Pt_9) ◽  
pp. 2303-2306 ◽  
Author(s):  
Matthias Sipiczki
Keyword(s):  
Yeast Species ◽  
Large Subunit ◽  
Culture Collection ◽  
Rrna Genes ◽  
Nucleotide Substitutions ◽  
D2 Domain ◽  
Anamorphic Yeast ◽  
18S Rrna Genes ◽  
Large Subunit Rrna ◽  
Species Being

Five strains of a previously uncharacterized anamorphic, methanol-assimilating yeast species are described here, for which the name Candida borneonana is proposed. The strains were isolated from fruit waste collected in markets in Brunei, Borneo. The sequences of the D1/D2 domains of the large subunit rRNA genes, the internal transcribed spacer (ITS) regions and the 18S rRNA genes were identical between the isolates and differed from the corresponding sequences of all previously described yeast species. Phylogenetic analysis of these sequences showed that the new species formed a cluster with species of the genus Kuraishia, the closest related species being K. capsulata (6 % nucleotide substitutions in the D1/D2 domain). The type strain, 11-487T, has been deposited in the Centralbureau voor Schimmelcultures (Utrecht, The Netherlands) as CBS 12507T, the Culture Collection of Yeasts (Bratislava, Slovakia) as CCY 29-182-1T and the National Collection of Agricultural and Industrial Microorganisms (Budapest, Hungary) as NCAIM Y.02008. Mycobank no. MB563710.

Pichia bruneiensis sp. nov., a biofilm-producing dimorphic yeast species isolated from flowers in Borneo

2012 ◽  
Vol 62 (Pt_12) ◽  
pp. 3099-3104 ◽  
Author(s):  
Matthias Sipiczki
Keyword(s):  
Yeast Species ◽  
Large Subunit ◽  
Culture Collection ◽  
Rrna Genes ◽  
Nucleotide Substitutions ◽  
D2 Domain ◽  
Close Phylogenetic Relationship ◽  
18S Rrna Genes ◽  
Micro Organisms ◽  
Large Subunit Rrna

Taxonomic analysis of five yeast strains isolated from Hibiscus flowers in Brunei (Borneo) is described. The strains represent a dimorphic, biofilm-producing, anamorphic budding yeast species for which the name Pichia bruneiensis is proposed. P. bruneiensis alternates between yeast and pseudohyphal modes of growth. The pseudohyphae form biofilms on the surface of liquid media and penetrate into solid substrates. The sequences of the D1/D2 domains of the large subunit rRNA genes, the internal transcribed spacer (ITS) regions and the 18S rRNA genes were identical in the five strains and indicated a close phylogenetic relationship with teleomorph species of the genus Pichia. In a phylogenetic analysis of these sequences, the closest relative of the new species was Pichia fermentans (6 % nucleotide substitutions and indels in the D1/D2 domain). The type strain is 11-485T and has been deposited in the Centralbureau voor Schimmelcultures (Utrecht, the Netherlands) as CBS 12611T, the National Collection of Agricultural and Industrial Micro-organisms (Budapest, Hungary) as NCAIM Y.02019T and the Culture Collection of Yeasts (Bratislava, Slovakia) as CCY 29-189-1T. Mycobank no. MB800537.

scholarly journals Hannaella siamensis sp. nov. and Hannaella phetchabunensis sp. nov., two new anamorphic basidiomycetous yeast species isolated from plants

2015 ◽  
Vol 65 (Pt_4) ◽  
pp. 1297-1303 ◽  
Author(s):  
Rungluk Kaewwichian ◽  
Sasitorn Jindamorakot ◽  
Somjit Am-In ◽  
Matthias Sipiczki ◽  
Savitree Limtong
Keyword(s):  
Type Strain ◽  
Yeast Species ◽  
Novel Species ◽  
Large Subunit ◽  
Its Region ◽  
Dead Leaf ◽  
Rrna Gene ◽  
Nucleotide Substitutions ◽  
Anamorphic Yeast ◽  
Large Subunit Rrna

Eight strains, representing two novel anamorphic yeast species, consisted of five strains isolated from the external surfaces of rice leaves (DMKU-RP72T, DMKU-RP109, DMKU-RP119, YE-124 and YE-156) and one from a corn leaf (DMKU-CP430T)4 collected in Thailand, and one strain isolated from each of a composite flower (11-1114) and a fallen dead leaf (12-301); the latter two were collected in Belize. On the basis of sequence analysis of the D1/D2 region of the large subunit rRNA gene and the internal transcribed spacer (ITS) region, they were suggested to be two novel species of the genus Hannaella. Seven strains (DMKU-RP72T, DMKU-RP109, DMKU-RP119, YE-124, YE-156, 11-1114 and 12-301) differed from each other by 0–3 nt substitutions in the D1/D2 region and by 0–1 nt substitutions in the ITS region. In terms of pairwise sequence similarities of the D1/D2 region these seven strains were closest to Hannaella zeae, but with 1.2–1.7 % (7–9) nucleotide substitutions. The sequences of the ITS region of these seven strains differed from H. zeae by 3.7–3.9 % (16–17) nucleotide substitutions. Therefore, they were assigned to a single novel species and the name Hannaella siamensis sp. nov. has been proposed. The type strain is DMKU-RP72T ( = BCC 69493T = NBRC 110425T = CBS 13533T). Strain DMKU-CP430T represents the second novel species and was also most closely related to H. zeae, but with 1.0 % (6) nucleotide substitutions in the D1/D2 region and 3.2 % (14) nucleotide substitutions in the ITS region. It was assigned to the proposed novel species, Hannaella phetchabunensis sp. nov. (type strain DMKU-CP430T = BCC 69492T = NBRC 110424T = CBS 13386T).

scholarly journals Hannaella phyllophila sp. nov., a basidiomycetous yeast species associated with plants in Thailand and Taiwan

2015 ◽  
Vol 65 (Pt_7) ◽  
pp. 2135-2140 ◽  
Author(s):  
Janjira Surussawadee ◽  
Sasitorn Jindamorakot ◽  
Takashi Nakase ◽  
Ching-Fu Lee ◽  
Savitree Limtong
Keyword(s):  
Yeast Species ◽  
Novel Species ◽  
Large Subunit ◽  
Its Region ◽  
Rrna Genes ◽  
Rrna Gene ◽  
Nucleotide Substitutions ◽  
Lsu Rrna Gene ◽  
Anamorphic Yeast ◽  
Lsu Rrna

Five strains representing one novel anamorphic yeast species were isolated from plant leaves collected in Thailand (strains DMKU-SP186T, ST-111 and ST-201) and Taiwan (strains FN20L02 and SM13L16). On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics and sequence analysis of the D1/D2 region of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region, they were assigned to a single novel species of the genus Hannaella. The sequences of the D1/D2 regions of the LSU rRNA genes of four of the strains (DMKU-SP186T, ST-111, FN20L02 and SM13L16) were identical, while differing from strain ST-201 by 2 substitutions and 2 gaps. The nucleotide sequence of the ITS regions of the five strains differed from each other by between 0 and 3 nucleotide substitutions. The novel species was most closely related to Hannaella luteola, but showed 1.0–1.3 % nucleotide substitutions (between 6 substitutions out of 568–606 nt and 8 substitutions, and 2 gaps out of 597 nt) in the D1/D2 region of the LSU rRNA gene and 1.4–2.0 % nucleotide substitutions (6–9 substitutions out of 435 nt) in the ITS region. Ballistospores were produced by three of the strains on cornmeal agar at 15 and 20 °C after 4 weeks, while H. luteola did not produce ballistospores. The name Hannaella phyllophila sp. nov. is proposed. The type strain is DMKU-SP186T ( = BCC 69500T = NBRC 110428T = CBS 13921T).

scholarly journals Metschnikowia laotica f.a., sp. nov., a dimorphic, pigment-producing yeast species isolated from fruit

2014 ◽  
Vol 64 (Pt_6) ◽  
pp. 1847-1852 ◽  
Author(s):  
Matthias Sipiczki
Keyword(s):  
Geographical Origin ◽  
Yeast Species ◽  
Novel Species ◽  
Large Subunit ◽  
Quinic Acid ◽  
Rrna Genes ◽  
Brown Pigment ◽  
The Novel ◽  
18S Rrna Genes ◽  
Large Subunit Rrna

Eight strains with identical sequences of the D1/D2 domains of the large subunit rRNA genes were isolated from fallen fruits in two distant localities in Laos. These strains represent a novel dimorphic budding yeast species producing invasive pseudohyphae and a brown pigment when growing on media containing quinic acid as the sole carbon source or tryptophan as the sole nitrogen source. Phylogenetic analysis of the sequences of the D1/D2 domains, the internal transcribed spacer (ITS) regions and the 18S rRNA genes placed the novel species in the Metschnikowia clade close to Candida torresii, Metschnikowia drosophilae and C andida danieliae. The taxonomic name Metschnikowia laotica f.a., sp. nov., reflecting the geographical origin of the isolates, is proposed for the novel species. The type strain is 11-524T ( = CBS 12961T = NCAIM Y.02124T = CCY 64-4-1T). The Mycobank number is MB 807383.

scholarly journals Cryptococcus taeanensis sp. nov., a new anamorphic basidiomycetous yeast isolated from a salt farm

2005 ◽  
Vol 55 (3) ◽  
pp. 1365-1368 ◽  
Author(s):  
Kee-Sun Shin ◽  
Yong-Ha Park ◽  
Dong-Jin Park ◽  
Chang-Jin Kim
Keyword(s):  
Yeast Species ◽  
Large Subunit ◽  
Its Region ◽  
Yeast Identification ◽  
Domain Sequence ◽  
D2 Domain ◽  
Molecular Phylogenetic ◽  
Anamorphic Yeast ◽  
Physiological Tests ◽  
Large Subunit Rrna

Cryptococcus taeanensis, a new anamorphic yeast species originating from a salt farm on the Taean peninsula in Korea, is described. Strain 3-12T grew by budding, contained ubiquinone Q-10 and xylose in cell hydrolysates, utilized d-glucuronate and did not ferment d-glucose. A molecular phylogenetic analysis based on the large-subunit rRNA D1/D2 domain and ITS region sequences placed C. taeanensis near Auriculibuller fuscus and Bullera japonica, recently proposed taxa of the Tremellales. However, these species were distinguishable based on standard physiological tests used for yeast identification, with characteristics including the assimilation of l-sorbose, absence of ballistoconidia, no arbutin hydrolysis and no growth in the presence of 0·01 % cycloheximide. The isolate exhibited the typical physiology of the genus Cryptococcus Vuillemin, but its large-subunit rRNA D1/D2 domain sequence was clearly distinct from previously described species in the genus. Therefore, on the basis of these results, Cryptococcus taeanensis sp. nov. is proposed; the type strain is 3-12T (=KCTC 17149T=CBS 9742T).

scholarly journals Candida andamanensis sp. nov., Candida laemsonensis sp. nov. and Candida ranongensis sp. nov., anamorphic yeast species isolated from estuarine waters in a Thai mangrove forest

2011 ◽  
Vol 61 (2) ◽  
pp. 454-461 ◽  
Author(s):  
Somjit Am-In ◽  
Savitree Limtong ◽  
Wichien Yongmanitchai ◽  
Sasitorn Jindamorakot
Keyword(s):  
Phylogenetic Analysis ◽  
Sequence Analysis ◽  
Type Strain ◽  
Mangrove Forest ◽  
Yeast Species ◽  
Novel Species ◽  
Large Subunit ◽  
Rrna Gene ◽  
D2 Domain ◽  
Large Subunit Rrna

Five strains (RV5T, RV140, R31T, RS17 and RS28T) representing three novel anamorphic ascomycetous yeast species were isolated by membrane filtration from estuarine waters collected from a mangrove forest in Laem Son National Park, Ranong Province, Thailand, on different occasions. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, sequence analysis of the D1/D2 domain of the large-subunit rRNA gene and the internal transcribed spacer region and phylogenetic analysis, three strains were found to represent two novel Candida species. Two strains (RV5T and RV140) represented a single novel species, for which the name Candida laemsonensis sp. nov. is proposed. The type strain is RV5T (=BCC 35154T =NBRC 105873T =CBS 11419T). Strain R31T was assigned to a novel species that was named Candida andamanensis sp. nov. (type strain R31T =BCC 25965T =NBRC 103862T =CBS 10859T). On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, sequence analysis of the D1/D2 domain of the large-subunit rRNA gene and phylogenetic analysis, strains RS17 and RS28T represented another novel species of Candida, for which the name Candida ranongensis sp. nov. is proposed. The type strain is RS28T (=BCC 25964T =NBRC 103861T =CBS 10861T).

scholarly journals Clavispora santaluciae f.a., sp. nov., a novel ascomycetous yeast species isolated from grapes

2020 ◽  
Vol 70 (12) ◽  
pp. 6307-6312
Author(s):  
João Drumonde-Neves ◽  
Neža Čadež ◽  
Yazmid Reyes-Domínguez ◽  
Andreas Gallmetzer ◽  
Dorit Schuller# ◽  
...  
Keyword(s):  
Yeast Species ◽  
Large Subunit ◽  
Its Region ◽  
Rrna Gene ◽  
Drosophila Suzukii ◽  
Nucleotide Substitutions ◽  
Ascomycetous Yeast ◽  
D2 Domain ◽  
Lsu Rrna Gene ◽  
Additional Strain

During a study of yeast diversity in Azorean vineyards, four strains were isolated which were found to represent a novel yeast species based on the sequences of the internal transcribed spacer (ITS) region (ITS1-5.8S–ITS2) and of the D1/D2 domain of the large subunit (LSU) rRNA gene, together with their physiological characteristics. An additional strain isolated from Drosophila suzukii in Italy had identical D1/D2 sequences and very similar ITS regions (five nucleotide substitutions) to the Azorean strains. Phylogenetic analysis using sequences of the ITS region and D1/D2 domain showed that the five strains are closely related to Clavispora lusitaniae, although with 56 nucleotide differences in the D2 domain. Intraspecies variation revealed between two and five nucleotide differences, considering the five strains of Clavispora santaluciae. Some phenotypic discrepancies support the separation of the new species from their closely related ones, such as the inability to grow at temperatures above 35 °C, to produce acetic acid and the capacity to assimilate starch. Neither conjugations nor ascospore formation were observed in any of the strains. The name Clavispora santaluciae f.a., sp. nov., is proposed to accommodate the above noted five strains (holotype, CBS 16465T; MycoBank no., MB 835794).

scholarly journals Description of four Apiotrichum and two Cutaneotrichosporon species isolated from guano samples from bat-inhabited caves in Japan

2020 ◽  
Vol 70 (8) ◽  
pp. 4458-4469
Author(s):  
Masako Takashima ◽  
Sanae Kurakado ◽  
Otomi Cho ◽  
Ken Kikuchi ◽  
Junta Sugiyama ◽  
...  
Keyword(s):  
New Species ◽  
Yeast Species ◽  
Large Subunit ◽  
Biochemical Properties ◽  
Rrna Genes ◽  
New Yeast Species ◽  
Rrna Gene ◽  
D2 Domain ◽  
Lsu Rrna ◽  
New Yeast

Four new yeast species belonging to the genus Apiotrichum and two new yeast species belonging to Cutaneotrichosporon are described for strains isolated from guano samples from bat-inhabited caves in Japan. In 2005, we reported these isolates as Trichosporon species based on sequence analyses of the D1/D2 domain of large subunit (LSU) rRNA genes according to available basidiomycetous yeast classification criteria; however, to date, they have not been officially published as new species with descriptions. Their phylogenetic positions have been reanalysed based on comparison of internal transcribed spacer (ITS) region sequences (including the 5.8S rRNA gene) and the D1/D2 domain of the LSU rRNA gene with those of known species; we confirmed clear separation from previously described species. Physiological and biochemical properties of the isolates also suggest their distinctiveness. Therefore, we describe Apiotrichum akiyoshidainum (holotype JCM 12595T), Apiotrichum chiropterorum (JCM 12594T), Apiotrichum coprophilum (JCM 12596T), Apiotrichum otae (JCM 12593T), Cutaneotrichosporon cavernicola (JCM 12590T) and Cutaneotrichosporon middelhovenii (JCM 12592T) as new species. C. cavernicola showed particularly distinctive morphology including large inflated anomalous cells on the hyphae and germination from the cells, although clear clamp connections on the hyphae were not confirmed. Further study is needed to elucidate the morph of this species.

scholarly journals Zygotorulaspora cariocana sp. nov., a yeast species isolated from tree bark in Brazil

2020 ◽  
Vol 70 (4) ◽  
pp. 2677-2681
Author(s):  
Juliana D. Moreira ◽  
Ana Raquel O. Santos ◽  
Fernanda L. C. Oliveira ◽  
Raquel M. Cadete ◽  
Ana Luiza Freire ◽  
...  
Keyword(s):  
Yeast Species ◽  
Novel Species ◽  
Large Subunit ◽  
Sequence Divergence ◽  
Amazon Rainforest ◽  
Tree Bark ◽  
Rrna Gene ◽  
The Novel ◽  
Nucleotide Substitutions ◽  
Large Subunit Rrna

Six strains of a novel yeast species were isolated from tree bark collected in the Atlantic Forest and the Amazon Rainforest in Brazil. Analyses of the sequences of D1/D2 domains of the large subunit rRNA gene showed that the strains belong to a species in the genus Zygotorulaspora. The species differed by 5.54 % sequence divergence (25 substitutions and five indels out of 542 bp) in the D1/D2 sequences from Zygotorulaspora mrakii, its closest relative. The ITS sequence of the type strain of the novel species differs by 27–69 nucleotide substitutions/indels from the other Zygotorulaspora species. The novel species is able to grow on trehalose, maltose, l-sorbose, inulin and at 37 °C, which are negative in Z. mrakii. The name Zygotorulaspora cariocana sp. nov. is proposed. The holotype of Z. cariocana sp. nov. is CBS 16118T. The MycoBank number is MB 833702.

Kloeckera taiwanica sp. nov., an ascomycetous apiculate yeast species isolated from mushroom fruiting bodies

2012 ◽  
Vol 62 (Pt_6) ◽  
pp. 1434-1437 ◽  
Author(s):  
Chin-Feng Chang ◽  
Li-Yin Huang ◽  
Shan-Fu Chen ◽  
Ching-Fu Lee
Keyword(s):  
Type Strain ◽  
Yeast Species ◽  
Novel Species ◽  
Large Subunit ◽  
Distinct Species ◽  
Rrna Gene ◽  
Sporulation Medium ◽  
Yeast Strains ◽  
D2 Domain ◽  
Large Subunit Rrna

Three apiculate yeast strains, EJ7M09T, GJ5M15 and GJ15M04, isolated from mushrooms in Taiwan were found to represent a novel species of the genus Kloeckera. The phylogenetically closest relative of this novel species is Hanseniaspora occidentalis, but the type strain of H. occidentalis differed by 4.6 % divergence (25 substitutions; 5 gaps) in the sequence of the D1/D2 domain of the large subunit rRNA gene. This difference clearly suggests that the three strains represent a distinct species. As none of the strains that were examined in this study produced ascospores or exhibited conjugation on common sporulation medium either alone or in a pairwise mixture, this species could be considered as an anamorphic member of the genus Hanseniaspora, and a novel species, Kloeckera taiwanica sp. nov., is proposed, with EJ7M09T ( = BCRC 23182T = CBS 11434T) as the type strain.

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