scholarly journals Description of four Apiotrichum and two Cutaneotrichosporon species isolated from guano samples from bat-inhabited caves in Japan

2020 ◽  
Vol 70 (8) ◽  
pp. 4458-4469
Author(s):  
Masako Takashima ◽  
Sanae Kurakado ◽  
Otomi Cho ◽  
Ken Kikuchi ◽  
Junta Sugiyama ◽  
...  
Keyword(s):  
New Species ◽  
Yeast Species ◽  
Large Subunit ◽  
Biochemical Properties ◽  
Rrna Genes ◽  
New Yeast Species ◽  
Rrna Gene ◽  
D2 Domain ◽  
Lsu Rrna ◽  
New Yeast

Four new yeast species belonging to the genus Apiotrichum and two new yeast species belonging to Cutaneotrichosporon are described for strains isolated from guano samples from bat-inhabited caves in Japan. In 2005, we reported these isolates as Trichosporon species based on sequence analyses of the D1/D2 domain of large subunit (LSU) rRNA genes according to available basidiomycetous yeast classification criteria; however, to date, they have not been officially published as new species with descriptions. Their phylogenetic positions have been reanalysed based on comparison of internal transcribed spacer (ITS) region sequences (including the 5.8S rRNA gene) and the D1/D2 domain of the LSU rRNA gene with those of known species; we confirmed clear separation from previously described species. Physiological and biochemical properties of the isolates also suggest their distinctiveness. Therefore, we describe Apiotrichum akiyoshidainum (holotype JCM 12595T), Apiotrichum chiropterorum (JCM 12594T), Apiotrichum coprophilum (JCM 12596T), Apiotrichum otae (JCM 12593T), Cutaneotrichosporon cavernicola (JCM 12590T) and Cutaneotrichosporon middelhovenii (JCM 12592T) as new species. C. cavernicola showed particularly distinctive morphology including large inflated anomalous cells on the hyphae and germination from the cells, although clear clamp connections on the hyphae were not confirmed. Further study is needed to elucidate the morph of this species.

scholarly journals Papiliotrema maritimi f.a. sp. nov., a new tremellaceous yeast species associated to macrophytes in a Marshland of South Brazil

2020 ◽  
Author(s):  
Mauricio Ramírez-Castrillón ◽  
Fernanda Fraga Gomes ◽  
Andrea Formoso de Souza ◽  
Belize Rodrigues Leite ◽  
Danielle Machado Pagani ◽  
...  
Keyword(s):  
Yeast Species ◽  
Large Subunit ◽  
Its Region ◽  
New Yeast Species ◽  
Rrna Gene ◽  
Phylogenetic Species Concept ◽  
D2 Domain ◽  
Lsu Rrna Gene ◽  
New Yeast ◽  
South Brazil

AbstractOne new yeast species, Papiliotrema maritimi sp. nov., is being proposed to be suitable into the Rhynchogastremataceae family, belonging to the Tremellales clade. This new species is related to six others from the Papiliotrema genus: P. taeanensis, P. siamense, P. perniciosus, P. nemorosus, P. bandonii, P. japonica and P. fuscus. The novel species is proposed based on the phylogenetic species concept through analysis of the D1/D2 region, part of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region. A total of 11 strains of Papiliotrema maritimi sp. nov. were obtained from macrophytes leaves collected in south Brazil. Papiliotrema maritimi sp. nov. differs by 12, 15, 25, 25, 25 and 29 substitutions in the D1/D2 domain from the related species P. fuscus, P. japonica, P. siamense, P. nemorosus, P. bandonii, and P. perniciosus, respectively. Concerning the ITS region, there are 11 substitutions and 52 or more substitutions when compared to P. teanensis and its closest relatives. The type strain of Papiliotrema maritimi sp. nov. is UFMG-CM-Y6048. The MycoBank number for Papiliotrema maritimi sp. nov. is MB 835603.

Kodamaea kakaduensisandCandida tolerans, two new ascomycetous yeast species from AustralianHibiscusflowers

1999 ◽  
Vol 45 (2) ◽  
pp. 172-177 ◽  
Author(s):  
Marc-André Lachance ◽  
Jane M Bowles ◽  
William T Starmer ◽  
J Stuart F. Barker
Keyword(s):  
Ribosomal Dna ◽  
Yeast Species ◽  
Large Subunit ◽  
New Yeast Species ◽  
Ascomycetous Yeast ◽  
Carbon And Nitrogen ◽  
D2 Domain ◽  
New Yeast ◽  
Type Strains ◽  
Candida Mogii

Two new yeast species were isolated from flowers of Hibiscus species in Eastern and Northern Australia. Kodamaea kakaduensis is heterothallic, haploid, and similar to other Kodamaea species and to Candida restingae. Buds are often produced on short protuberances, and a true mycelium is formed. The new species differs from others by the assimilation of trehalose, melezitose, and xylitol, and is reproductively isolated. The cells of Candida tolerans are small and a pseudomycelium is formed. The carbon and nitrogen assimilation pattern is reminiscent of that of Zygosaccharomyces rouxii but the two are not closely related. Sequences of the D1/D2 domain of large subunit ribosomal DNA confirm the membership of K. kakaduensis in the genus Kodamaea and indicate that C. tolerans belongs to the Clavispora-Metschnikowia clade, with a moderate relatedness to Candida mogii. The type strains are: K. kakaduensis, UWO(PS)98-119.2 (h+, holotype, CBS 8611) and UWO(PS)98-117.1 (h-, isotype, CBS 8612); and C. tolerans, UWO(PS)98-115.5 (CBS 8613).Key words: Kodamaea, Candida, new yeast species, ribosomal DNA, whole-cell PCR.

Candida ipomoeae, a new yeast species related to large-spored Metschnikowia species

1998 ◽  
Vol 44 (8) ◽  
pp. 718-722 ◽  
Author(s):  
Marc-André Lachance ◽  
Carlos A Rosa ◽  
William T Starmer ◽  
Jane M Bowles
Keyword(s):  
Yeast Species ◽  
Large Subunit ◽  
Single Species ◽  
Morning Glory ◽  
New Yeast Species ◽  
Nutritional Profile ◽  
D2 Domain ◽  
Sap Beetles ◽  
Agar Media ◽  
New Yeast

Numerous strains of an unusual asexual yeast species were isolated from flowers of morning glory (Ipomoea spp., Convolvulaceae) and associated drosophilids and sap beetles of the genus Conotelus sampled in Hawaii and in Brazil. The nutritional profile of this yeast is similar to those of Metschnikowia hawaiiensis and Metschnikowia continentalis, which share the same habitats. The cells are large, hydrophobic, and tend to remain attached after budding, causing the colonies on agar media to have a convoluted appearance, reminiscent of popcorn. The sequences of the D1/D2 domain of large subunit rDNAs of strains from three different localities confirmed that a single species is involved, and that it is related to large-spored Metschnikowia species. The type strain is UWO(PS)91-672.1 (CBS 8466).Key words: Candida ipomoeae, yeast, new species, rDNA.

Three new ascomycetous yeasts from insect-associated arboreal habitats

1999 ◽  
Vol 46 (1) ◽  
pp. 50-58
Author(s):  
C P Kurtzman
Keyword(s):  
New Species ◽  
Mating Type ◽  
Ribosomal Dna ◽  
Large Subunit ◽  
Sequence Divergence ◽  
New Yeast Species ◽  
Ascomycetous Yeast ◽  
D2 Domain ◽  
New Yeast ◽  
Ascomycetous Yeasts

A new species of Pichia and two new species of Candida are described and were determined to be genetically isolated from all other currently recognized ascomycetous yeasts from their sequence divergence in the species-variable D1/D2 domain of large subunit (26S) ribosomal DNA. The three species were primarily isolated from the frass of wood-boring insects living in pine and spruce trees. The new species and their type strains are the following: Pichia ramenticola NRRL YB-1985 (CBS 8699), mating type alpha (NRRL YB-3835, CBS 8700, mating type a), Candida piceae NRRL YB-2107 (CBS 8701), and Candida wyomingensis NRRL YB-2152 (CBS 8703). Pichia ramenticola and C. piceae assimilate methanol as a carbon source; P. ramenticola is the first known heterothallic ascomycetous yeast to utilize this compound.Key words: Pichia, Candida, new yeast species, ribosomal DNA, molecular systematics, methanol utilization.

Three new insect-associated species of the yeast genus Candida

1998 ◽  
Vol 44 (10) ◽  
pp. 965-973 ◽  
Author(s):  
C P Kurtzman ◽  
C J Robnett
Keyword(s):  
New Species ◽  
Ribosomal Dna ◽  
Large Subunit ◽  
Sequence Divergence ◽  
New Yeast Species ◽  
D2 Domain ◽  
Three New Species ◽  
New Yeast ◽  
Ascomycetous Yeasts ◽  
Associated Species

Three new species of Candida are described that were determined to be genetically isolated from all other currently accepted ascomycetous yeasts based on their sequence divergence in the species-variable D1/D2 domain of large subunit (26S) ribosomal DNA. One of the species was isolated from an ambrosia beetle, whereas the other two were from the frass of wood-boring beetle larvae. The new species and their type strains are the following: Candida ontarioensis NRRL YB-1246 (CBS 8502), Candida tammaniensis NRRL Y-8257 (CBS 8504), and Candida trypodendroni NRRL Y-6488 (CBS 8505).Key words: Candida, new yeast species, ribosomal DNA, molecular systematics.

scholarly journals BIODIVERSITY OF YEASTS ISOLATED FROM CON DAO ISLAND – BA RIA VUNG TAU

2018 ◽  
Vol 55 (1A) ◽  
pp. 99
Author(s):  
Dao Thi Luong
Keyword(s):  
New Species ◽  
Yeast Species ◽  
Candida Rugosa ◽  
New Yeast Species ◽  
Yeast Diversity ◽  
26S Rdna ◽  
Diverse Species ◽  
Yeast Strains ◽  
D2 Domain ◽  
New Yeast

During the study on yeast diversity from natural samples in Viet Nam, we have collected 38 samples of flowers, fruits, leaves, litter and soils in Con Dao Island and isolated 54 yeast strains. They were identified based on morphology observation and sequences of D1/D2 domain of 26S rDNA. The results showed that they belonged to 13 genera, 28 species, of which 15 strains were suspected as 13 new species. Thirty-nine strains were identified as Ascomycetes belonging 7 genera, 11 species, and the fifteen remaining strains belonged to Basidomycetes, 6 genera and 13 species. The results also showed that four Ascomycetous and nine Basidomycetous isolated were suspected to be new species. The results also showed that Candida was the genus with highest number isolates- 26 strains, and half of these were Candida rugosa. The most diverse species in this study was found in Cryptococcus genus with 7 species from 8 isolates only, and all of them were suspected to be new species. This study also proved that the samples had differences in yeast diversity and species composition among them. Therefore, Con Dao Island is multi potential to discover and publish new yeast species in Vietnam. 

scholarly journals Hannaella phyllophila sp. nov., a basidiomycetous yeast species associated with plants in Thailand and Taiwan

2015 ◽  
Vol 65 (Pt_7) ◽  
pp. 2135-2140 ◽  
Author(s):  
Janjira Surussawadee ◽  
Sasitorn Jindamorakot ◽  
Takashi Nakase ◽  
Ching-Fu Lee ◽  
Savitree Limtong
Keyword(s):  
Yeast Species ◽  
Novel Species ◽  
Large Subunit ◽  
Its Region ◽  
Rrna Genes ◽  
Rrna Gene ◽  
Nucleotide Substitutions ◽  
Lsu Rrna Gene ◽  
Anamorphic Yeast ◽  
Lsu Rrna

Five strains representing one novel anamorphic yeast species were isolated from plant leaves collected in Thailand (strains DMKU-SP186T, ST-111 and ST-201) and Taiwan (strains FN20L02 and SM13L16). On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics and sequence analysis of the D1/D2 region of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region, they were assigned to a single novel species of the genus Hannaella. The sequences of the D1/D2 regions of the LSU rRNA genes of four of the strains (DMKU-SP186T, ST-111, FN20L02 and SM13L16) were identical, while differing from strain ST-201 by 2 substitutions and 2 gaps. The nucleotide sequence of the ITS regions of the five strains differed from each other by between 0 and 3 nucleotide substitutions. The novel species was most closely related to Hannaella luteola, but showed 1.0–1.3 % nucleotide substitutions (between 6 substitutions out of 568–606 nt and 8 substitutions, and 2 gaps out of 597 nt) in the D1/D2 region of the LSU rRNA gene and 1.4–2.0 % nucleotide substitutions (6–9 substitutions out of 435 nt) in the ITS region. Ballistospores were produced by three of the strains on cornmeal agar at 15 and 20 °C after 4 weeks, while H. luteola did not produce ballistospores. The name Hannaella phyllophila sp. nov. is proposed. The type strain is DMKU-SP186T ( = BCC 69500T = NBRC 110428T = CBS 13921T).

scholarly journals Occultifur tropicalis f.a., sp. nov., a novel cystobasidiomycetous yeast species isolated from tropical regions

2015 ◽  
Vol 65 (Pt_5) ◽  
pp. 1578-1582 ◽  
Author(s):  
Pannida Khunnamwong ◽  
Janjira Surussawadee ◽  
Sasitorn Jindamorakot ◽  
José R. A. Ribeiro ◽  
Allen N. Hagler ◽  
...  
Keyword(s):  
Sugar Cane ◽  
Yeast Species ◽  
Large Subunit ◽  
Its Region ◽  
Rrna Genes ◽  
Rrna Gene ◽  
Nucleotide Substitutions ◽  
Tropical Regions ◽  
Lsu Rrna Gene ◽  
Lsu Rrna

Five strains representing a single novel anamorphic yeast species were isolated from sugar cane. Two strains were from tissue (DMKU-SE38, DMKU-SE59T) and two from the external surface (DMKU-SP385, DMKU-SP403) of leaves collected in Thailand and the fifth (IMUFRJ 52020) from the rhizoplane of sugar cane in an organically cultivated field in Brazil. On the basis of sequence analysis of the D1/D2 region of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region, they were classified as representing a single species of the genus Occultifur. The sequences of the D1/D2 region of the LSU rRNA genes and the ITS regions of the five strains were either identical or differed from each other by only one nucleotide substitution. The novel species was related most closely to Occultifur brasiliensis f.a. CBS 12687T but with 0.7–1.0 % nucleotide substitutions (4–6 nt) in the D1/D2 region of the LSU rRNA gene and 2.5–2.7 % nucleotide substitutions (14–15 nt) in the ITS region. The name Occultifur tropicalis f.a., sp. nov. is proposed. The type strain is DMKU-SE59T ( = BCC 61184T = NBRC 109696T = CBS 13389 T).

scholarly journals Savitreea pentosicarens gen. nov., sp. nov., a yeast species in the family Saccharomycetaceae isolated from a grease trap

2020 ◽  
Vol 70 (11) ◽  
pp. 5665-5670
Author(s):  
Varunya Sakpuntoon ◽  
Jirameth Angchuan ◽  
Chanita Boonmak ◽  
Pannida Khunnamwong ◽  
Noémie Jacques ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Yeast Species ◽  
Sequence Divergence ◽  
Its Region ◽  
Rrna Gene ◽  
Nucleotide Substitutions ◽  
D2 Domain ◽  
The Family ◽  
Lsu Rrna Gene ◽  
Lsu Rrna

Two strains (DMKU-GTCP10-8 and CLIB 1740) representing a novel anamorphic yeast species were isolated from a grease sample collected from a grease trap in Thailand and from an unidentified fungus collected in French Guiana, respectively. On the basis of phylogenetic analysis based on the combined D1/D2 domain of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region, Lachancea fermentati CBS 707T was the closely related species with 12.8 % sequence divergence (70 nucleotide substitutions and three gaps in 571 nucleotides) and 28.1 % sequence divergence (93 nucleotide substitutions and 90 gaps in 651 nucleotides) in the D1/D2 domain of the LSU rRNA gene and the ITS region, respectively. Phylogenetic analysis based on the concatenated sequences of the five genes including the small subunit rRNA gene, the D1/D2 domain of the LSU rRNA gene, the ITS region, translation elongation factor-1 alpha (TEF1) and RNA polymerase II subunit 2 (RPB2) genes confirmed that the two strains (DMKU-GTCP10-8 and CLIB 1740) were well-separated from other described yeast genera in Saccharomycetaceae. Hence, Savitreea pentosicarens gen. nov., sp. nov. is proposed to accommodate these two strains as members of the family Saccharomycetaceae. The holotype is S. pentosicarens DMKU-GTCP10-8T (ex-type strain TBRC 12159=PYCC 8490; MycoBank number 835044).

Candida ecuadorensis sp. nov., an ascomycetous yeast species found in two separate regions of Ecuador

2013 ◽  
Vol 63 (Pt_1) ◽  
pp. 393-397 ◽  
Author(s):  
Stephen A. James ◽  
Enrique Javier Carvajal Barriga ◽  
Patricia Portero Barahona ◽  
Kathryn Cross ◽  
Christopher J. Bond ◽  
...  
Keyword(s):  
Type Strain ◽  
Mangrove Forest ◽  
Yeast Species ◽  
Novel Species ◽  
Large Subunit ◽  
Rrna Gene ◽  
Ascomycetous Yeast ◽  
Rotten Wood ◽  
D2 Domain ◽  
Lsu Rrna Gene

In the course of an on-going study aimed at cataloguing the natural yeast biodiversity found in Ecuador, two strains (CLQCA 13-025 and CLQCA 20-004T) were isolated from samples of cow manure and rotten wood collected in two separate provinces of the country (Orellana and Bolívar). These strains were found to represent a novel yeast species based on the sequences of their D1/D2 domain of the large-subunit (LSU) rRNA gene and their physiological characteristics. Phylogenetic analysis based on LSU D1/D2 sequences revealed this novel species to belong to the Metschnikowia clade and to be most closely related to Candida suratensis, a species recently discovered in a mangrove forest in Thailand. The species name of Candida ecuadorensis sp. nov. is proposed to accommodate these strains, with strain CLQCA 20-004T ( = CBS 12653T = NCYC 3782T) designated as the type strain.

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