scholarly journals Hannaella siamensis sp. nov. and Hannaella phetchabunensis sp. nov., two new anamorphic basidiomycetous yeast species isolated from plants

2015 ◽  
Vol 65 (Pt_4) ◽  
pp. 1297-1303 ◽  
Author(s):  
Rungluk Kaewwichian ◽  
Sasitorn Jindamorakot ◽  
Somjit Am-In ◽  
Matthias Sipiczki ◽  
Savitree Limtong
Keyword(s):  
Type Strain ◽  
Yeast Species ◽  
Novel Species ◽  
Large Subunit ◽  
Its Region ◽  
Dead Leaf ◽  
Rrna Gene ◽  
Nucleotide Substitutions ◽  
Anamorphic Yeast ◽  
Large Subunit Rrna

Eight strains, representing two novel anamorphic yeast species, consisted of five strains isolated from the external surfaces of rice leaves (DMKU-RP72T, DMKU-RP109, DMKU-RP119, YE-124 and YE-156) and one from a corn leaf (DMKU-CP430T)4 collected in Thailand, and one strain isolated from each of a composite flower (11-1114) and a fallen dead leaf (12-301); the latter two were collected in Belize. On the basis of sequence analysis of the D1/D2 region of the large subunit rRNA gene and the internal transcribed spacer (ITS) region, they were suggested to be two novel species of the genus Hannaella. Seven strains (DMKU-RP72T, DMKU-RP109, DMKU-RP119, YE-124, YE-156, 11-1114 and 12-301) differed from each other by 0–3 nt substitutions in the D1/D2 region and by 0–1 nt substitutions in the ITS region. In terms of pairwise sequence similarities of the D1/D2 region these seven strains were closest to Hannaella zeae, but with 1.2–1.7 % (7–9) nucleotide substitutions. The sequences of the ITS region of these seven strains differed from H. zeae by 3.7–3.9 % (16–17) nucleotide substitutions. Therefore, they were assigned to a single novel species and the name Hannaella siamensis sp. nov. has been proposed. The type strain is DMKU-RP72T ( = BCC 69493T = NBRC 110425T = CBS 13533T). Strain DMKU-CP430T represents the second novel species and was also most closely related to H. zeae, but with 1.0 % (6) nucleotide substitutions in the D1/D2 region and 3.2 % (14) nucleotide substitutions in the ITS region. It was assigned to the proposed novel species, Hannaella phetchabunensis sp. nov. (type strain DMKU-CP430T = BCC 69492T = NBRC 110424T = CBS 13386T).

Kazachstania rupicola sp. nov., a yeast species isolated from water tanks of a bromeliad in Brazil

2013 ◽  
Vol 63 (Pt_3) ◽  
pp. 1165-1168 ◽  
Author(s):  
Silvana Vilas Boas Safar ◽  
Fátima C. O. Gomes ◽  
Andréa R. Marques ◽  
Marc-André Lachance ◽  
Carlos A. Rosa
Keyword(s):  
Type Strain ◽  
Yeast Species ◽  
Novel Species ◽  
Large Subunit ◽  
Campo Rupestre ◽  
Rrna Gene ◽  
The Novel ◽  
Nucleotide Substitutions ◽  
Water Tanks ◽  
Large Subunit Rrna

Two isolates of a novel yeast species were obtained from water tanks (phytotelmata) of the bromeliad Vriesea minarum collected in a tableland (‘campo rupestre’) ecosystem in Brazil. The sequences of the D1/D2 domains of the large-subunit rRNA gene showed that this species is related to Kazachstania exigua and others, from which it differs by 8–10 nucleotide substitutions. The novel species Kazachstania rupicola sp. nov. is proposed to accommodate these isolates. The type strain is UFMG-BRO-80T ( = CBS 12684T  = CBMAI 1466T).

scholarly journals Yamadazyma insecticola f.a., sp. nov. and Yamadazyma epiphylla f.a., sp. nov., two novel yeast species

2015 ◽  
Vol 65 (Pt_4) ◽  
pp. 1290-1296 ◽  
Author(s):  
Sasitorn Jindamorakot ◽  
Somjit Am-In ◽  
Rungluk Kaewwichian ◽  
Savitree Limtong
Keyword(s):  
Type Strain ◽  
Yeast Species ◽  
Novel Species ◽  
Sequence Similarity ◽  
Large Subunit ◽  
Rrna Gene ◽  
Nucleotide Substitutions ◽  
Pairwise Sequence Similarity ◽  
Rice Leaves ◽  
Large Subunit Rrna

Two yeast strains representing two novel yeast species were isolated from frass of an unidentified insect (ST-78T) and the external surfaces of rice leaves (YE170T) collected in Thailand. The two strains were genetically, morphologically and phenotypically distinct from recognized species and were found to represent two novel species of the genus Yamadazyma although formation of ascospores was not observed. In terms of pairwise sequence similarity of the D1/D2 region of the large subunit rRNA gene, the closest relative of strain ST-78T was Candida lessepsii CBS 9941T but with 3.8 % nucleotide substitutions, while the closest relative of strain YE170T was strain ST-78T but with 4.3 % nucleotide substitutions. Analysis of the sequences of the internal transcribed spacer 1 and 2 (ITS1–2) regions revealed that strain ST-78 differed from C. lessepsii CBS 9941T by 8.8 % nucleotide substitutions and from strain YE170T by 9.4 % nucleotide substitutions. The result of pairwise sequence similarity of the D1/D2 and ITS1–2 regions together with phylogenetic analysis indicated that strains ST-78T and YE170T represented two novel species within the Yamadazyma clade. The names Yamadazyma insecticola f.a., sp. nov. (type strain ST-78T = BCC 8314T = NBRC 110421T = CBS 13382T; MycoBank no. MB810546) and Yamadazyma epiphylla f.a., sp. nov. (type strain YE170T = BCC 63466T = NBRC 110423T = CBS 13384T; MycoBank no. MB810547) are proposed for the two novel yeast species.

scholarly journals Barnettozyma siamensis f.a., sp. nov., a lipid-accumulating ascomycete yeast species

2014 ◽  
Vol 64 (Pt_9) ◽  
pp. 3053-3057 ◽  
Author(s):  
Pirapan Polburee ◽  
Wichien Yongmanitchai ◽  
Takao Ohashi ◽  
Kazuhito Fujiyama ◽  
Savitree Limtong
Keyword(s):  
Type Strain ◽  
Yeast Species ◽  
Novel Species ◽  
Large Subunit ◽  
Its Region ◽  
Rrna Gene ◽  
Nucleotide Substitutions ◽  
Isolation Technique ◽  
Lsu Rrna Gene ◽  
Lsu Rrna

Two strains, DMKU-UbN24(1)T and DMKU-CPN24(1), of a novel yeast species were obtained from soil and palm oil fruit, respectively, collected in Thailand by an enrichment isolation technique using a nitrogen-limited medium containing glycerol as the sole source of carbon. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics and sequence analysis of the D1/D2 region of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region, the two strains were found to represent a novel species of the genus Barnettozyma although the formation of ascospores was not observed. The novel species was related most closely to the type strain of Candida montana but differed by 5.4 % nucleotide substitutions in the D1/D2 region of the LSU rRNA gene and by 10.3–10.5 % nucleotide substitutions in the ITS region. The name Barnettozyma siamensis f.a., sp. nov. is proposed. The type strain is DMKU-UbN24(1)T ( = BCC 61189T = NBRC 109701T = CBS 13392T).

scholarly journals Hannaella phyllophila sp. nov., a basidiomycetous yeast species associated with plants in Thailand and Taiwan

2015 ◽  
Vol 65 (Pt_7) ◽  
pp. 2135-2140 ◽  
Author(s):  
Janjira Surussawadee ◽  
Sasitorn Jindamorakot ◽  
Takashi Nakase ◽  
Ching-Fu Lee ◽  
Savitree Limtong
Keyword(s):  
Yeast Species ◽  
Novel Species ◽  
Large Subunit ◽  
Its Region ◽  
Rrna Genes ◽  
Rrna Gene ◽  
Nucleotide Substitutions ◽  
Lsu Rrna Gene ◽  
Anamorphic Yeast ◽  
Lsu Rrna

Five strains representing one novel anamorphic yeast species were isolated from plant leaves collected in Thailand (strains DMKU-SP186T, ST-111 and ST-201) and Taiwan (strains FN20L02 and SM13L16). On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics and sequence analysis of the D1/D2 region of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region, they were assigned to a single novel species of the genus Hannaella. The sequences of the D1/D2 regions of the LSU rRNA genes of four of the strains (DMKU-SP186T, ST-111, FN20L02 and SM13L16) were identical, while differing from strain ST-201 by 2 substitutions and 2 gaps. The nucleotide sequence of the ITS regions of the five strains differed from each other by between 0 and 3 nucleotide substitutions. The novel species was most closely related to Hannaella luteola, but showed 1.0–1.3 % nucleotide substitutions (between 6 substitutions out of 568–606 nt and 8 substitutions, and 2 gaps out of 597 nt) in the D1/D2 region of the LSU rRNA gene and 1.4–2.0 % nucleotide substitutions (6–9 substitutions out of 435 nt) in the ITS region. Ballistospores were produced by three of the strains on cornmeal agar at 15 and 20 °C after 4 weeks, while H. luteola did not produce ballistospores. The name Hannaella phyllophila sp. nov. is proposed. The type strain is DMKU-SP186T ( = BCC 69500T = NBRC 110428T = CBS 13921T).

scholarly journals Ogataea mangiferae sp. nov., a methylotrophic yeast isolated from mango leaves

2015 ◽  
Vol 65 (Pt_6) ◽  
pp. 1855-1859 ◽  
Author(s):  
Ana Raquel O. Santos ◽  
Elisa S. Faria ◽  
Marc-André Lachance ◽  
Carlos A. Rosa
Keyword(s):  
Type Strain ◽  
Yeast Species ◽  
Novel Species ◽  
Mangifera Indica ◽  
Large Subunit ◽  
Its Region ◽  
Methylotrophic Yeast ◽  
Rrna Gene ◽  
The Novel ◽  
Mango Leaves

Five strains of a novel methanol-assimilating yeast species were isolated from mango (Mangifera indica) leaves collected at the campus of the Federal University of Minas Gerais in Brazil. The sequences of the internal transcribed spacer (ITS) region and the D1/D2 domains of the large subunit of the rRNA gene showed that this species belongs to the Ogataea clade and is related to O. allantospora, O. chonburiensis, O. dorogensis, O. kodamae, O. paradorogensis and Candida xyloterini (Ogataea clade). The novel species differs in the D1/D2 domains of the large subunit of the rRNA gene by 12 to 40 substitutions from these Ogataea species. The name Ogataea mangiferae sp. nov. is proposed for this novel species. The type strain of Ogataea mangiferae sp. nov. is UFMG-CM-Y253T ( = CBS 13492T). The Mycobank number is MB 811646.

scholarly journals Candida andamanensis sp. nov., Candida laemsonensis sp. nov. and Candida ranongensis sp. nov., anamorphic yeast species isolated from estuarine waters in a Thai mangrove forest

2011 ◽  
Vol 61 (2) ◽  
pp. 454-461 ◽  
Author(s):  
Somjit Am-In ◽  
Savitree Limtong ◽  
Wichien Yongmanitchai ◽  
Sasitorn Jindamorakot
Keyword(s):  
Phylogenetic Analysis ◽  
Sequence Analysis ◽  
Type Strain ◽  
Mangrove Forest ◽  
Yeast Species ◽  
Novel Species ◽  
Large Subunit ◽  
Rrna Gene ◽  
D2 Domain ◽  
Large Subunit Rrna

Five strains (RV5T, RV140, R31T, RS17 and RS28T) representing three novel anamorphic ascomycetous yeast species were isolated by membrane filtration from estuarine waters collected from a mangrove forest in Laem Son National Park, Ranong Province, Thailand, on different occasions. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, sequence analysis of the D1/D2 domain of the large-subunit rRNA gene and the internal transcribed spacer region and phylogenetic analysis, three strains were found to represent two novel Candida species. Two strains (RV5T and RV140) represented a single novel species, for which the name Candida laemsonensis sp. nov. is proposed. The type strain is RV5T (=BCC 35154T =NBRC 105873T =CBS 11419T). Strain R31T was assigned to a novel species that was named Candida andamanensis sp. nov. (type strain R31T =BCC 25965T =NBRC 103862T =CBS 10859T). On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, sequence analysis of the D1/D2 domain of the large-subunit rRNA gene and phylogenetic analysis, strains RS17 and RS28T represented another novel species of Candida, for which the name Candida ranongensis sp. nov. is proposed. The type strain is RS28T (=BCC 25964T =NBRC 103861T =CBS 10861T).

scholarly journals Zygotorulaspora cariocana sp. nov., a yeast species isolated from tree bark in Brazil

2020 ◽  
Vol 70 (4) ◽  
pp. 2677-2681
Author(s):  
Juliana D. Moreira ◽  
Ana Raquel O. Santos ◽  
Fernanda L. C. Oliveira ◽  
Raquel M. Cadete ◽  
Ana Luiza Freire ◽  
...  
Keyword(s):  
Yeast Species ◽  
Novel Species ◽  
Large Subunit ◽  
Sequence Divergence ◽  
Amazon Rainforest ◽  
Tree Bark ◽  
Rrna Gene ◽  
The Novel ◽  
Nucleotide Substitutions ◽  
Large Subunit Rrna

Six strains of a novel yeast species were isolated from tree bark collected in the Atlantic Forest and the Amazon Rainforest in Brazil. Analyses of the sequences of D1/D2 domains of the large subunit rRNA gene showed that the strains belong to a species in the genus Zygotorulaspora. The species differed by 5.54 % sequence divergence (25 substitutions and five indels out of 542 bp) in the D1/D2 sequences from Zygotorulaspora mrakii, its closest relative. The ITS sequence of the type strain of the novel species differs by 27–69 nucleotide substitutions/indels from the other Zygotorulaspora species. The novel species is able to grow on trehalose, maltose, l-sorbose, inulin and at 37 °C, which are negative in Z. mrakii. The name Zygotorulaspora cariocana sp. nov. is proposed. The holotype of Z. cariocana sp. nov. is CBS 16118T. The MycoBank number is MB 833702.

Kloeckera taiwanica sp. nov., an ascomycetous apiculate yeast species isolated from mushroom fruiting bodies

2012 ◽  
Vol 62 (Pt_6) ◽  
pp. 1434-1437 ◽  
Author(s):  
Chin-Feng Chang ◽  
Li-Yin Huang ◽  
Shan-Fu Chen ◽  
Ching-Fu Lee
Keyword(s):  
Type Strain ◽  
Yeast Species ◽  
Novel Species ◽  
Large Subunit ◽  
Distinct Species ◽  
Rrna Gene ◽  
Sporulation Medium ◽  
Yeast Strains ◽  
D2 Domain ◽  
Large Subunit Rrna

Three apiculate yeast strains, EJ7M09T, GJ5M15 and GJ15M04, isolated from mushrooms in Taiwan were found to represent a novel species of the genus Kloeckera. The phylogenetically closest relative of this novel species is Hanseniaspora occidentalis, but the type strain of H. occidentalis differed by 4.6 % divergence (25 substitutions; 5 gaps) in the sequence of the D1/D2 domain of the large subunit rRNA gene. This difference clearly suggests that the three strains represent a distinct species. As none of the strains that were examined in this study produced ascospores or exhibited conjugation on common sporulation medium either alone or in a pairwise mixture, this species could be considered as an anamorphic member of the genus Hanseniaspora, and a novel species, Kloeckera taiwanica sp. nov., is proposed, with EJ7M09T ( = BCRC 23182T = CBS 11434T) as the type strain.

scholarly journals Papiliotrema siamense f.a., sp. nov., a yeast species isolated from plant leaves

2014 ◽  
Vol 64 (Pt_9) ◽  
pp. 3058-3062 ◽  
Author(s):  
Janjira Surussawadee ◽  
Pannida Khunnamwong ◽  
Nantana Srisuk ◽  
Savitree Limtong
Keyword(s):  
Type Strain ◽  
External Surface ◽  
Novel Species ◽  
Large Subunit ◽  
Its Region ◽  
Rrna Gene ◽  
Plant Leaves ◽  
Nucleotide Substitutions ◽  
Lsu Rrna Gene ◽  
Lsu Rrna

Two strains representing a novel species were isolated from the external surface of a sugar cane leaf (DMKU-SP85T) and tissue of a rice leaf (DMKU-RE97) collected in Thailand. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, and sequence analysis of the D1/D2 region of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region, the two strains were determined to represent a novel species of the genus Papiliotrema although sexual reproduction was not observed. The sequences of the D1/D2 region of the LSU rRNA gene and ITS region of the two strains were identical, but differed from those of the type strain of Cryptococcus nemorosus by 0.6 % nucleotide substitutions (four nucleotide substitutions out of 597 nucleotides) in the D1/D2 region of the LSU rRNA gene and 1.8 % nucleotide substitutions (nine nucleotide substitutions out of 499 nucleotides) in the ITS region. The name Papiliotrema siamense f.a., sp. nov. is proposed. The type strain is DMKU-SP85T( = BCC 69499T = CBS 13330T).

scholarly journals Lindnera rhizosphaerae sp. nov., a yeast species isolated from rhizospheric soil

2011 ◽  
Vol 61 (4) ◽  
pp. 985-988 ◽  
Author(s):  
M. Cecilia Mestre ◽  
Carlos A. Rosa ◽  
Sonia B. Fontenla
Keyword(s):  
Type Strain ◽  
Yeast Species ◽  
Novel Species ◽  
Large Subunit ◽  
Rrna Gene ◽  
Native Forest ◽  
The Novel ◽  
Nothofagus Pumilio ◽  
Rhizospheric Soil ◽  
Large Subunit Rrna

Two strains of a novel yeast species were isolated from ectomycorrhizal Nothofagus pumilio rhizospheric soil in a native forest of Patagonia, Argentina. Analysis of the D1/D2 large-subunit rRNA gene sequences indicated that the novel species belongs to the recently described genus Lindnera. The name Lindnera rhizosphaerae sp. nov. is proposed to accommodate these isolates, and the type strain is CRUB 1796T ( = CBS 11400T  = JCM 16499T).

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