scholarly journals Surface LSP1 is a phenotypic marker distinguishing human classical versus monocyte-derived dendritic cells

2019 ◽  
Author(s):  
Sandrine Moutel ◽  
Anne Beugnet ◽  
Aurélie Schneider ◽  
Bérangère Lombard ◽  
Damarys Loew ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Surface Marker ◽  
Mononuclear Phagocytes ◽  
Single Domain ◽  
Phenotypic Marker ◽  
Phenotypic Markers ◽  
Monocytes And Macrophages ◽  
Cellular Domain ◽  
Single Domain Antibodies

AbstractHuman mononuclear phagocytes comprise several subsets of dendritic cells (DCs), monocytes and macrophages. Distinguishing one population from another is challenging, especially in inflammed tissues, due to the promiscuous expression of phenotypic markers. Using a synthetic library of humanized llama single domain antibodies, we identified a novel surface marker for human naturally-occuring monocyte-derived DCs. Our antibody targets an extra-cellular domain of LSP-1, specifically on monocyte-derived DCs, but not on monocytes, macrophages or classical DCs. Using this antibody, we provide evidence that the recently described blood DC3 population does not correspond to circulating monocyte-derived DCs. Our findings will pave the way for a better characterization of human mononuclear phagocytes in pathological settings.

scholarly journals Generation and characterization of CD1d-specific single-domain antibodies with distinct functional features

Immunology ◽  
2016 ◽  
Vol 149 (1) ◽  
pp. 111-121 ◽  
Author(s):  
Roeland Lameris ◽  
Renée C. G. de Bruin ◽  
Paul M. P. van Bergen en Henegouwen ◽  
Henk M. Verheul ◽  
Sonja Zweegman ◽  
...  
Keyword(s):  
Single Domain ◽  
Single Domain Antibodies ◽  
Functional Features

scholarly journals Selection and characterization of single domain antibodies against human CD20

2016 ◽  
Vol 78 ◽  
pp. 146-154 ◽  
Author(s):  
Jinny L. Liu ◽  
Dan Zabetakis ◽  
Ellen R. Goldman ◽  
George P. Anderson
Keyword(s):  
Single Domain ◽  
Single Domain Antibodies

scholarly journals Discovery and characterization of single-domain antibodies for polymeric Ig receptor-mediated mucosal delivery of biologics

mAbs ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 1708030
Author(s):  
Bharathikumar Vellalore Maruthachalam ◽  
Adam Zwolak ◽  
Xiefan Lin-Schmidt ◽  
Edward Keough ◽  
Ninkka Tamot ◽  
...  
Keyword(s):  
Single Domain ◽  
Mucosal Delivery ◽  
Single Domain Antibodies

scholarly journals Selection and Characterization of Anti-Dengue NS1 Single Domain Antibodies

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Lisa C. Shriver-Lake ◽  
Jinny L. Liu ◽  
Dan Zabetakis ◽  
Victor A. Sugiharto ◽  
Cheng-Rei Lee ◽  
...  
Keyword(s):  
Single Domain ◽  
Single Domain Antibodies

scholarly journals Selection of Single-Domain Antibodies towards Western Equine Encephalitis Virus

Antibodies ◽  
2018 ◽  
Vol 7 (4) ◽  
pp. 44 ◽  
Author(s):  
Jinny Liu ◽  
Lisa Shriver-Lake ◽  
Dan Zabetakis ◽  
Ellen Goldman ◽  
George Anderson
Keyword(s):  
Phage Display Library ◽  
Peripheral Blood Lymphocytes ◽  
Encephalitis Virus ◽  
Single Domain ◽  
Equine Encephalitis Virus ◽  
Western Equine Encephalitis Virus ◽  
Recognition Elements ◽  
Single Domain Antibodies ◽  
Western Equine Encephalitis

In this work, we describe the selection and characterization of single-domain antibodies (sdAb) towards the E2/E3E2 envelope protein of the Western equine encephalitis virus (WEEV). Our purpose was to identify novel recognition elements which could be used for the detection, diagnosis, and perhaps treatment of western equine encephalitis (WEE). To achieve this goal, we prepared an immune phage display library derived from the peripheral blood lymphocytes of a llama that had been immunized with an equine vaccine that includes killed WEEV (West Nile Innovator + VEWT). This library was panned against recombinant envelope (E2/E3E2) protein from WEEV, and seven representative sdAb from the five identified sequence families were characterized. The specificity, affinity, and melting point of each sdAb was determined, and their ability to detect the recombinant protein in a MagPlex sandwich immunoassay was confirmed. Thus, these new binders represent novel recognition elements for the E2/E3E2 proteins of WEEV that are available to the research community for further investigation into their applicability for use in the diagnosis or treatment of WEE.

Selection and characterization of protective anti-chikungunya virus single domain antibodies

2019 ◽  
Vol 105 ◽  
pp. 190-197 ◽  
Author(s):  
Jinny L. Liu ◽  
Lisa C. Shriver-Lake ◽  
Dan Zabetakis ◽  
George P. Anderson ◽  
Ellen R. Goldman
Keyword(s):  
Chikungunya Virus ◽  
Single Domain ◽  
Single Domain Antibodies

Characterization of Single-Domain Antibodies with an Engineered Disulfide Bond

2012 ◽  
pp. 417-429
Author(s):  
Greg Hussack ◽  
C. Roger MacKenzie ◽  
Jamshid Tanha
Keyword(s):  
Disulfide Bond ◽  
Single Domain ◽  
Single Domain Antibodies

scholarly journals Isolation and characterization of camelid single-domain antibodies against HER2

2018 ◽  
Vol 11 (1) ◽  
Author(s):  
Greg Hussack ◽  
Shalini Raphael ◽  
Michael J. Lowden ◽  
Kevin A. Henry
Keyword(s):  
Single Domain ◽  
Isolation And Characterization ◽  
Single Domain Antibodies

Isolation and characterization of shark single domain antibodies capable of binding salmonid alphavirus

2019 ◽  
Vol 91 ◽  
pp. 426-427
Author(s):  
D. Munir ◽  
H. Dooley ◽  
E. Munro ◽  
C. Secombes
Keyword(s):  
Single Domain ◽  
Isolation And Characterization ◽  
Single Domain Antibodies

scholarly journals Selection and characterization of llama single domain antibodies against N-terminal huntingtin

2014 ◽  
Vol 36 (3) ◽  
pp. 429-434 ◽  
Author(s):  
Menno H. Schut ◽  
Barry A. Pepers ◽  
Rinse Klooster ◽  
Silvère M. van der Maarel ◽  
Mohamed el Khatabi ◽  
...  
Keyword(s):  
Single Domain ◽  
Single Domain Antibodies
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