scholarly journals Regulation of Aromatic Amino Acid Biosynthesis in Higher Plants

1972 ◽  
Vol 49 (1) ◽  
pp. 52-57 ◽  
Author(s):  
D. G. Gilchrist ◽  
Terry S. Woodin ◽  
Marilyn L. Johnson ◽  
T. Kosuge
Keyword(s):  
Amino Acid ◽  
Aromatic Amino Acid ◽  
Higher Plants ◽  
Amino Acid Biosynthesis ◽  
Acid Biosynthesis ◽  
Aromatic Amino Acid Biosynthesis

Regulation of aromatic amino acid biosynthesis in higher plants

1974 ◽  
Vol 164 (1) ◽  
pp. 95-105 ◽  
Author(s):  
David G. Gilchrist ◽  
Tsune Kosuge
Keyword(s):  
Amino Acid ◽  
Aromatic Amino Acid ◽  
Higher Plants ◽  
Amino Acid Biosynthesis ◽  
Acid Biosynthesis ◽  
Aromatic Amino Acid Biosynthesis

scholarly journals Microbial Origin of Plant-Type 2-Keto-3-Deoxy-d-arabino-Heptulosonate 7-Phosphate Synthases, Exemplified by the Chorismate- and Tryptophan-Regulated Enzyme from Xanthomonas campestris

2001 ◽  
Vol 183 (13) ◽  
pp. 4061-4070 ◽  
Author(s):  
Guillermo Gosset ◽  
Carol A. Bonner ◽  
Roy A. Jensen
Keyword(s):  
Amino Acid ◽  
Aromatic Amino Acid ◽  
Xanthomonas Campestris ◽  
Higher Plants ◽  
Feedback Inhibition ◽  
Amino Acid Biosynthesis ◽  
Partial Purification ◽  
Initial Reaction ◽  
Acid Biosynthesis ◽  
Aromatic Amino Acid Biosynthesis

ABSTRACT Enzymes performing the initial reaction of aromatic amino acid biosynthesis, 2-keto-3-deoxy-d-arabino-heptulosonate 7-phosphate (DAHP) synthases, exist as two distinct homology classes. The three classic Escherichia coli paralogs are AroAI proteins, but many members of theBacteria possess the AroAII class of enzyme, sometimes in combination with AroAI proteins. AroAII DAHP synthases until now have been shown to be specifically dedicated to secondary metabolism (e.g., formation of ansamycin antibiotics or phenazine pigment). In contrast, here we show that the Xanthomonas campestris AroAII protein functions as the sole DAHP synthase supporting aromatic amino acid biosynthesis. X. campestris AroAII was cloned in E. coli by functional complementation, and genes corresponding to two possible translation starts were expressed. We developed a 1-day partial purification method (>99%) for the unstable protein. The recombinant AroAII protein was found to be subject to an allosteric pattern of sequential feedback inhibition in which chorismate is the prime allosteric effector.l-Tryptophan was found to be a minor feedback inhibitor. An N-terminal region of 111 amino acids may be located in the periplasm since a probable inner membrane-spanning region is predicted. Unlike chloroplast-localized AroAII of higher plants, X. campestris AroAII was not hysteretically activated by dithiols. Compared to plant AroAII proteins, differences in divalent metal activation were also observed. Phylogenetic tree analysis shows that AroAII originated within theBacteria domain, and it seems probable that higher-plant plastids acquired AroAII from a gram-negative bacterium via endosymbiosis. The X. campestris AroAII protein is suggested to exemplify a case of analog displacement whereby an ancestral aroA I species was discarded, with thearoA II replacement providing an alternative pattern of allosteric control. Three subgroups of AroAIIproteins can be recognized: a large, central group containing the plant enzymes and that from X. campestris, one defined by a three-residue deletion near the conserved KPRS motif, and one possessing a larger deletion further downstream.

scholarly journals Regulation of Aromatic Amino Acid Biosynthesis in Higher Plants

1974 ◽  
Vol 249 (21) ◽  
pp. 6842-6848
Author(s):  
Oenes C. Huisman ◽  
Tsune Kosuge
Keyword(s):  
Amino Acid ◽  
Aromatic Amino Acid ◽  
Higher Plants ◽  
Amino Acid Biosynthesis ◽  
Acid Biosynthesis ◽  
Aromatic Amino Acid Biosynthesis

Biochemical Interface Between Aromatic Amino Acid Biosynthesis and Secondary Metabolism

1989 ◽  
pp. 89-107 ◽  
Author(s):  
Roy A. Jensen ◽  
Paul Morris ◽  
Carol Bonner ◽  
Lolita O. Zamir
Keyword(s):  
Amino Acid ◽  
Secondary Metabolism ◽  
Aromatic Amino Acid ◽  
Amino Acid Biosynthesis ◽  
Acid Biosynthesis ◽  
Aromatic Amino Acid Biosynthesis

Aromatic amino acid biosynthesis in Alcaligenes eutrophus H16

1975 ◽  
Vol 103 (1) ◽  
pp. 141-149 ◽  
Author(s):  
B�rbel Friedrich ◽  
H. G. Schlegel
Keyword(s):  
Amino Acid ◽  
Aromatic Amino Acid ◽  
Alcaligenes Eutrophus ◽  
Amino Acid Biosynthesis ◽  
Acid Biosynthesis ◽  
Aromatic Amino Acid Biosynthesis ◽  
Alcaligenes Eutrophus H16
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