Probabilistic terrain modeling of mobile robots for outdoor applications using a scanning laser

Author(s):  
Bo Zhou ◽  
Kun Qian ◽  
Xudong Ma ◽  
Xianzhong Dai
Author(s):  
JS Deitch ◽  
KL Smith ◽  
JW Swann ◽  
JN Turner

Neurons labeled with horseradish peroxidase and reacted with diaminobenzidine (DAB) can be imaged using a confocal scanning laser microscope (CSLM) in the reflection mode. In contrast to fluorescent markers, the DAB reaction product is thought to be stable and can be observed by both light and electron microscopy. We have investigated the sensitivity of the DAB reaction product to laser irradiation, and present the spectrophotometric properties of DAB before and after exposure in the CSLM.Pyramidal neurons in slices of rat hippocampus were injected with biocytin (a biotin-lysine complex), fixed overnight in 4% paraformaldehyde, and vibratome sectioned at 75 μm. Biocytin was detected with avidin-HRP (1:200) in 0.5% Triton X-100, incubated in DAB (0.5 mg/ml) with or without 0.04% nickel ammonium sulfate (Ni), dehydrated, and imaged in a Bio Rad MRC-500 CSLM with an argon ion laser (488 and 514 nm). Spectrophotometric measurements of the soma were made on a Zeiss microspectrophotometer, as a function of laser exposure (100-1000 scans) and staining protocol.


2012 ◽  
Vol 132 (3) ◽  
pp. 381-388
Author(s):  
Takaaki Imaizumi ◽  
Hiroyuki Murakami ◽  
Yutaka Uchimura

2006 ◽  
Vol 65 (3) ◽  
pp. 229-241
Author(s):  
S. F. Yatsun ◽  
F. K. Freire ◽  
V. S. Dyshenko ◽  
O. A. Shadrina
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