This paper addresses the performance of pseudorandom Binary Amplitude Shift Keying (BASK) emission sequences for an ultrasonic multi-life location system in rescue robot. To improve the performance of multi-life location system, the BASK modulation were used to construct the spread spectrum emission sequences for ultrasonic sensors. Correlation technique in the receiving circuit was used to de-spread. Good correlation characteristics, i.e., sharp autocorrelation and flat cross-correlation, can help eliminate crosstalk between multichannel ultrasonic sensors. To improve the echo signal correlation effect, genetic algorithm was applied to optimize pseudorandom m sequences. In simulated post-earthquake environment, experimental verification of ultrasonic multi-life location system consisting of eight-channel SensComp 600 series electrostatic sensors with optimized BASK emission sequences was carried out. The experimental results demonstrate that the ultrasonic multi-life location system with the proposed method can realize multi-life location simultaneously.