According to industry and agriculture domain which applied temperature sensor need calibration. It is proposed the temperature calibration system design which based on the environmental of the LabVIEW programming to complete the parameter calibration and characteristics analysis of temperature sensor. The design communicates to serial bus by VISA programming interface to complete setting the temperature of the FLUKE9171 temperature calibrator, and get the temperature informations of the Multi-channel DS18B20 temperature sensor. It demarcates the collected data by using the method of the curve fitting, to complete the accurate calibration of the the temperature which collected by temperature sensor. The whole system design is parameters custom, intelligent, self-adaption, which has been applied on a sensor calibration system.