CLASSIFICATION OF HUMAN SERUM PREALBUMINS AFTER STARCH GEL ELECTROPHORESIS

1966 ◽  
Vol 68 (3) ◽  
pp. 434-438 ◽  
Author(s):  
MAGNE K. FAGERHOL ◽  
MIKAEL BRAEND
Keyword(s):  
Human Serum ◽  
Gel Electrophoresis ◽  
Starch Gel Electrophoresis ◽  
Starch Gel

Ultrasonic Effects on lsoenzymes

1966 ◽  
Vol 12 (4) ◽  
pp. 181-186 ◽  
Author(s):  
Clyde A Dubbs
Keyword(s):  
Human Serum ◽  
Ultrasonic Treatment ◽  
Gel Electrophoresis ◽  
Serum Proteins ◽  
Serum Cholinesterase ◽  
Starch Gel Electrophoresis ◽  
Selective Activation ◽  
Intracellular Enzymes ◽  
Starch Gel

Abstract Several significant effects of ultrasonic treatment on human serum cholinesterase and aminopeptidase isoenzymes and on other serum proteins have been found by starch gel electrophoresis. The selective activation of one cholinesterase isoenzyme is especially striking. These effects must be considered when ultrasonic treatment is used for the extraction of intracellular enzymes. When the effects are appreciated, ultrasonics should provide a valuable tool for isoenzyme research.

Human serum α-1-lipoprotein patterns revealed by starch gel electrophoresis

Lipids ◽  
1968 ◽  
Vol 3 (5) ◽  
pp. 420-424 ◽  
Author(s):  
Louis Cohen ◽  
Juliana Djordjevich
Keyword(s):  
Human Serum ◽  
Gel Electrophoresis ◽  
Starch Gel Electrophoresis ◽  
Starch Gel

scholarly journals HETEROGENEITY OF THE INHERITED GROUP-SPECIFIC COMPONENT OF HUMAN SERUM

1964 ◽  
Vol 120 (1) ◽  
pp. 83-91 ◽  
Author(s):  
Alexander G. Bearn ◽  
F. David Kitchin ◽  
Barbara H. Bowman
Keyword(s):  
Human Serum ◽  
Gel Electrophoresis ◽  
Polypeptide Chain ◽  
Buffer System ◽  
Starch Gel Electrophoresis ◽  
Specific Component ◽  
Agar Gel ◽  
Main Band ◽  
Starch Gel ◽  
Normal Human

Heterogeneity of the group-specific (Gc) components in normal human serum has been demonstrated by the use of a lithium borate buffer system in conventional vertical starch gel electrophoresis and by prolonged immunoelectrophoresis in agar gel. In both Gc 1-1 and Gc 2-2 phenotypes a protein component migrates ahead of the main band. Immunological evidence indicates that the faster migrating band contains Gc specificity. The possibility that the two electrophoretically distinct Gc components share a common polypeptide chain is discussed.

THE ASSOCIATION OF I131-LABELLED THYROXINE AND TRIIODOTHYRONINE WITH SERUM PROTEINS AFTER STARCH GEL ELECTROPHORESIS

1965 ◽  
Vol 43 (9) ◽  
pp. 1477-1487 ◽  
Author(s):  
Amy Britton ◽  
Brian R. Webster ◽  
Calvin Ezrin ◽  
Robert Volpe
Keyword(s):  
Human Serum ◽  
Gel Electrophoresis ◽  
Serum Proteins ◽  
Borate Buffer ◽  
Starch Gel Electrophoresis ◽  
Thyroxine Binding Globulin ◽  
Starch Gel

The binding of I131-labelled triiodothyronine (T3) and 1-thyroxine (T4) with the proteins of human serum has been investigated by means of vertical starch gel electrophoresis in borate buffer at pH 8.6. T3 was found to bind largely to thyroxine-binding globulin (TBG) with very little association with albumin, whereas T4 was associated with TBG, albumin, and thyroxine-binding prealbumin (TBPA). Sera from normal persons were shown to have binding capacities for added thyroxine of 25 μg T4 per 100 ml for TBG, and 40 μg T4 per 100 ml for TBPA.

scholarly journals 365 BIOSYSTEMATICAL CLASSIFICATION OF GENUS ROSA USING ISOZYME POLYMORPHISMS

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 483c-483 ◽  
Author(s):  
Young-ju Kim ◽  
David H. Byrne
Keyword(s):  
Gel Electrophoresis ◽  
Cultivar Identification ◽  
Isozyme Analysis ◽  
Lithium Borate ◽  
Starch Gel Electrophoresis ◽  
Enzyme Systems ◽  
Starch Gel ◽  
Isozyme Variability ◽  
Species Groups

Isozyme analysis has been used for cultivar identification, but little has been done with the genus Rosa. One hundred and sixty rose accessions (species, cultivars, and hybrids) were characterized for isozyme phenotypes using starch gel electrophoresis. Six enzyme systems were stained on three electrode buffer systems. ACP, MDH, and 6PGD were run on morpholine citrate (pH 6.1) and histidine (pH 5.7), PGI and PGM were run on histidine (pH 5.7) and lithium borate (pH 8.3), and SKDH was run on morpholine citrate (PH 6.1) and lithium borate (PH 8.3). The most variable isozymes were MDH and 6PGD. MDH and 6PGD revealed 10 and 9 bands, respectively. This study showed that isozyme variability exists in roses and can be useful in their classification into species groups.

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