Identification of human serum proteins binding iron, copper, and thyroid hormones by starch gel electrophoresis

1959 ◽  
Vol 15 (7) ◽  
pp. 281-284 ◽  
Author(s):  
A. C. Allison
Keyword(s):  
Thyroid Hormones ◽  
Human Serum ◽  
Gel Electrophoresis ◽  
Serum Proteins ◽  
Starch Gel Electrophoresis ◽  
Iron Copper ◽  
Starch Gel ◽  
Human Serum Proteins

Ultrasonic Effects on lsoenzymes

1966 ◽  
Vol 12 (4) ◽  
pp. 181-186 ◽  
Author(s):  
Clyde A Dubbs
Keyword(s):  
Human Serum ◽  
Ultrasonic Treatment ◽  
Gel Electrophoresis ◽  
Serum Proteins ◽  
Serum Cholinesterase ◽  
Starch Gel Electrophoresis ◽  
Selective Activation ◽  
Intracellular Enzymes ◽  
Starch Gel

Abstract Several significant effects of ultrasonic treatment on human serum cholinesterase and aminopeptidase isoenzymes and on other serum proteins have been found by starch gel electrophoresis. The selective activation of one cholinesterase isoenzyme is especially striking. These effects must be considered when ultrasonic treatment is used for the extraction of intracellular enzymes. When the effects are appreciated, ultrasonics should provide a valuable tool for isoenzyme research.

THE ASSOCIATION OF I131-LABELLED THYROXINE AND TRIIODOTHYRONINE WITH SERUM PROTEINS AFTER STARCH GEL ELECTROPHORESIS

1965 ◽  
Vol 43 (9) ◽  
pp. 1477-1487 ◽  
Author(s):  
Amy Britton ◽  
Brian R. Webster ◽  
Calvin Ezrin ◽  
Robert Volpe
Keyword(s):  
Human Serum ◽  
Gel Electrophoresis ◽  
Serum Proteins ◽  
Borate Buffer ◽  
Starch Gel Electrophoresis ◽  
Thyroxine Binding Globulin ◽  
Starch Gel

The binding of I131-labelled triiodothyronine (T3) and 1-thyroxine (T4) with the proteins of human serum has been investigated by means of vertical starch gel electrophoresis in borate buffer at pH 8.6. T3 was found to bind largely to thyroxine-binding globulin (TBG) with very little association with albumin, whereas T4 was associated with TBG, albumin, and thyroxine-binding prealbumin (TBPA). Sera from normal persons were shown to have binding capacities for added thyroxine of 25 μg T4 per 100 ml for TBG, and 40 μg T4 per 100 ml for TBPA.

Starch-gel Electrophoresis of Pig Serum Proteins

Nature ◽  
1963 ◽  
Vol 197 (4873) ◽  
pp. 1201-1201 ◽  
Author(s):  
R. K. SCOPES
Keyword(s):  
Gel Electrophoresis ◽  
Serum Proteins ◽  
Starch Gel Electrophoresis ◽  
Starch Gel ◽  
Pig Serum
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