Comprehensive, serologically equivalent DNA typing for HLA-B by PCR using sequence-specific primers (PCR-SSP)

1995 ◽  
Vol 45 (2) ◽  
pp. 81-90 ◽  
Author(s):  
M. Bunce ◽  
G. C. Fanning ◽  
K. I. Welsh
Keyword(s):  
Dna Typing ◽  
Specific Primers

HLA-B locus DNA typing using sequence specific primers in allele or group specific ARMS/PCR

1994 ◽  
Vol 39 (2) ◽  
pp. 134
Author(s):  
A.M. Sadler ◽  
P. Krausa ◽  
S.G.E. Marsh ◽  
F. Petronzelli ◽  
J.G. Bodner ◽  
...  
Keyword(s):  
Dna Typing ◽  
Specific Primers ◽  
B Locus ◽  
Arms Pcr

Low-resolution DNA typing for HLA-B using sequence-specific primers in allele- or group-specific ARMS/PCR

1994 ◽  
Vol 44 (3) ◽  
pp. 148-154 ◽  
Author(s):  
A. M. Sadler ◽  
F. Petronzelli ◽  
P. Krausa ◽  
S. G. E. Marsh ◽  
M. G. Guttridge ◽  
...  
Keyword(s):  
Dna Typing ◽  
Low Resolution ◽  
Specific Primers ◽  
Arms Pcr

Phototyping: comprehensive DNA typing for HLA-A, B, C, DRB1, DRB3, DRB4, DRB5 & DQB1 by PCR with 144 primer mixes utilizing sequence-specific primers (PCR-SSP)

1995 ◽  
Vol 46 (5) ◽  
pp. 355-367 ◽  
Author(s):  
M. Bunce ◽  
C. M. O'Neill ◽  
M. C. N. M. Barnardo ◽  
P. Krausa ◽  
M. J. Browning ◽  
...  
Keyword(s):  
Dna Typing ◽  
Specific Primers

scholarly journals DNA Typing for SLA-DRB1 by PCR Using Sequence-Specific Primers (PCR-SSP)

1997 ◽  
Vol 68 (4) ◽  
pp. 389-398
Author(s):  
Kazuo KAWAKAMI ◽  
Kumiko TAKEDA ◽  
Akira ONISHI ◽  
Emiko NAKAJIMA ◽  
Masanori KOMATSU
Keyword(s):  
Dna Typing ◽  
Specific Primers

DNA typing for BoLA class I using sequence-specific primers (PCR-SSP)

1998 ◽  
Vol 25 (5) ◽  
pp. 365-370 ◽  
Author(s):  
S. A. Ellis ◽  
K. A. Staines ◽  
M. J. Stear ◽  
E. J. Hensen ◽  
W. I. Morrison
Keyword(s):  
Dna Typing ◽  
Class I ◽  
Specific Primers ◽  
Bola Class

DNA typing for BoLA class I using sequence-specific primers (PCR-SSP)

1998 ◽  
Vol 25 (5) ◽  
pp. 365-370 ◽  
Author(s):  
S. A. Ellis ◽  
K. A. Staines ◽  
M. J. Stear ◽  
E. J. Hensen ◽  
W. I. Morrison
Keyword(s):  
Dna Typing ◽  
Class I ◽  
Specific Primers ◽  
Bola Class

Localization of the Br Polymorphism on a 144 bp Exon of the GPIa Gene and Its Application in Platelet DNA Typing

1994 ◽  
Vol 71 (05) ◽  
pp. 651-654 ◽  
Author(s):  
Rainer Kalb ◽  
Sentot Santoso ◽  
Katja Unkelbach ◽  
Volker Kiefel ◽  
Christian Mueller-Eckhardt
Keyword(s):  
Genomic Organization ◽  
Fragment Length Polymorphism ◽  
Human Platelet ◽  
Dna Typing ◽  
Rflp Analysis ◽  
Serological Typing ◽  
Allele Specific ◽  
Polymerase Chain ◽  
Alloimmune Thrombocytopenia ◽  
Rflp Typing

SummaryAlloimmunization against the human platelet alloantigen system Br (HPA-5) is the second most common cause of neonatal alloimmune thrombocytopenia (NAIT) in Caucasian populations. We have recently shown that a single base polymorphism at position 1648 on platelet mRNA coding for GPIa results in an aminoacid substitution at position 505 on the mature GPIa which is associated with the two serological defined Br phenotypes.Since DNA-typing of platelet alloantigens offers possibilities for useful clinical applications, we designed genomic DNA-based restriction fragment length polymorphism (RFLP) typing for Br alloantigens. To establish this technique we analyzed the genomic organization of GPIa adjacent to the polymorphic base. Using the polymerase chain reaction (PCR) of blood cell DNA we have identified two introns (approximately 1.7 and 1.9 kb) flanking a 144 bp coding sequence of the GPIa gene encompassing the polymorphic base 1648. Based on the in- tron sequence, a PCR primer was constructed to amplify a 274 bp fragment which was used for allele-specific RFLP to determine the Br genotypes. The results of RFLP analysis using Mnll endonuclease obtained from 15 donors (2 Br37*, 2 Br^ and 11 Brb/b) correlate perfectly with serological typing by monoclonal antibody-specific immobilization of platelet antigens (MAIPA) assay.

A Hemi-nested, Allele Specific, Whole Blood PCR Assay for the Detection of the Factor V Leiden Mutation

1997 ◽  
Vol 77 (06) ◽  
pp. 1154-1155 ◽  
Author(s):  
Gary D Sinclair ◽  
Sandra Low ◽  
Man-Chiu Poon
Keyword(s):  
Whole Blood ◽  
Protein C ◽  
Activated Protein C ◽  
Factor V Leiden ◽  
Restriction Enzyme Digestion ◽  
Factor V ◽  
Pcr Assay ◽  
Specific Primers ◽  
Factor V Leiden Mutation ◽  
Allele Specific

SummaryWe describe a novel hemi-nested, allele specific whole blood PCR assay for detection of the factor V Leiden mutation associated with the plasma defect, activated protein C resistance. This assay utilizes 5 μl of whole blood without prior DNA extraction. The hemi-nested design, employing an outer primer pair in combination with nested, allele specific primers obviates the need for restriction enzyme digestion. PCR reactions are analysed directly on agarose or polyacrylamide minigels. The assay confirmed the genotypes of 50 individuals previously categorized by PCR and Mnll digestion, and has been subsequently utilized in the genotyping of 445 individuals referred for thrombosis studies.

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