Isolation and characterization of monodeamidated derivatives of bovine pancreatic Ribonuclease A

2009 ◽  
Vol 23 (5) ◽  
pp. 494-505 ◽  
Author(s):  
Yeldur P. Venkatesh ◽  
Paul J. Vithayathil
Biochemistry ◽  
1997 ◽  
Vol 36 (42) ◽  
pp. 12683-12699 ◽  
Author(s):  
John H. Laity ◽  
Cathy C. Lester ◽  
Sakurako Shimotakahara ◽  
Diane E. Zimmerman ◽  
Gaetano T. Montelione ◽  
...  

1963 ◽  
Vol 238 (2) ◽  
pp. 618-621 ◽  
Author(s):  
Arthur M. Crestfield ◽  
William H. Stein ◽  
Stanford Moore

1973 ◽  
Vol 248 (22) ◽  
pp. 7833-7837
Author(s):  
Ryoko Kobayashi ◽  
C.H.W. Hirs ◽  
With the technical assistance of Marie Hagenhuber

1990 ◽  
Vol 111 (4) ◽  
pp. 1639-1643 ◽  
Author(s):  
S C Ho ◽  
M Schindler ◽  
J L Wang

Extracts of Bradyrhizobium japonicum were fractionated on Sepharose columns covalently derivatized with lactose. Elution of the material that was specifically bound to the affinity column with lactose yielded a protein of Mr approximately 38,000. Isoelectric focusing of this sample yielded two spots with pI values of 6.4 and 6.8. This protein specifically bound to galactose-containing glycoconjugates, but did not bind either to glucose or mannose. Derivatives of galactose at the C-2 position showed much weaker binding; there was an 18-fold difference in the relative binding affinities of galactose versus N-acetyl-D-galactosamine. These results indicate that we have purified a newly identified carbohydrate-binding protein from Bradyrhizobium japonicum, that can exquisitely distinguish galactose from its derivatives at the C-2 position.


2011 ◽  
Vol 319 (1) ◽  
pp. 49-56 ◽  
Author(s):  
Hideaki Takusagawa ◽  
Shigefumi Yamamura ◽  
Shigeru Endo ◽  
Yoko Sugawara ◽  
Toshiyuki Inagaki ◽  
...  

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