Kinetic characterization of barley root plasma membrane-bound Ca2+- and Mg2+-dependent adenosine triphosphatase activities

1980 ◽  
Vol 50 (2) ◽  
pp. 183-193 ◽  
Author(s):  
CHARLES R. CALDWELL ◽  
ALFRED HAUG
Keyword(s):  
Plasma Membrane ◽  
Adenosine Triphosphatase ◽  
Barley Root ◽  
Kinetic Characterization ◽  
Membrane Bound ◽  
Root Plasma Membrane

scholarly journals PROBLEMS ASSOCIATED WITH THE DEMONSTRATION BY LEAD METHODS OF ADENOSINE TRIPHOSPHATASE ACTIVITY IN RESIDENT PERITONEAL MACROPHAGES AND EXUDATE MONOCYTES OF THE GUINEA PIG

1973 ◽  
Vol 21 (5) ◽  
pp. 488-498 ◽  
Author(s):  
R. E. POELMANN ◽  
W. T. DAEMS ◽  
E. J. VAN LOHUIZEN
Keyword(s):  
Plasma Membrane ◽  
Guinea Pig ◽  
Microscopic Study ◽  
Heterogeneous Nucleation ◽  
Adenosine Triphosphatase ◽  
Peritoneal Macrophages ◽  
Electron Microscopic ◽  
Adenosine Triphosphatase Activity ◽  
Eosinophilic Granulocytes ◽  
Membrane Bound

This cytochemical and electron microscopic study on peritoneal macrophages of the guinea pig has raised doubts concerning the validity of lead methods for the demonstration of plasma membrane-bound adenosine triphosphatase activity. The problems encountered are inherent in the use of lead ions as a capture reagent. The nonenzymatically formed precipitates reflect sites of heterogeneous nucleation specific for certain kinds of cells, e.g., resident peritoneal macrophages, eosinophilic granulocytes and, to a lesser degree, exudate monocytes. This type of precipitation is also catalyzed on the surface of nonbiologic matrices such as latex particles. Enzymatic processes may well occur, but they cannot be distinguished from nonenzymatic processes.

scholarly journals Purification and Characterization of Two Distinct NAD(P)H Dehydrogenases from Onion (Allium cepa L.) Root Plasma Membrane

1994 ◽  
Vol 106 (1) ◽  
pp. 87-96 ◽  
Author(s):  
A. Serrano ◽  
F. Cordoba ◽  
J. A. Gonzalez-Reyes ◽  
P. Navas ◽  
J. M. Villalba
Keyword(s):  
Plasma Membrane ◽  
Allium Cepa ◽  
Purification And Characterization ◽  
Allium Cepa L ◽  
Root Plasma Membrane
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