Site-directed Mutagenesis of Varicella-zoster Virus Thymidylate Synthase. Analysis of Two Highly Conserved Regions of the Enzyme

1995 ◽  
Vol 230 (2) ◽  
pp. 511-516 ◽  
Author(s):  
Patrick T. Harrison ◽  
James E. Scott ◽  
Michael J. Hutchinson ◽  
Russell Thompson
Keyword(s):  
Varicella Zoster Virus ◽  
Thymidylate Synthase ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Varicella Zoster ◽  
Conserved Regions

scholarly journals Structure of the Varicella Zoster Virus Thymidylate Synthase Establishes Functional and Structural Similarities as the Human Enzyme and Potentiates Itself as a Target of Brivudine

PLoS ONE ◽  
2015 ◽  
Vol 10 (12) ◽  
pp. e0143947 ◽  
Author(s):  
Kelly Hew ◽  
Sue-Li Dahlroth ◽  
Saranya Veerappan ◽  
Lucy Xin Pan ◽  
Tobias Cornvik ◽  
...  
Keyword(s):  
Varicella Zoster Virus ◽  
Thymidylate Synthase ◽  
Varicella Zoster ◽  
Human Enzyme

scholarly journals Development of Antibiotic-Overproducing Strains by Site-Directed Mutagenesis of the rpsL Gene in Streptomyces lividans

2003 ◽  
Vol 69 (7) ◽  
pp. 4256-4259 ◽  
Author(s):  
Yoshiko Okamoto-Hosoya ◽  
Susumu Okamoto ◽  
Kozo Ochi
Keyword(s):  
Ribosomal Protein ◽  
Antibiotic Production ◽  
Strain Improvement ◽  
Streptomyces Lividans ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Novel Mutations ◽  
Conserved Regions ◽  
Silent Genes ◽  
Ribosomal Protein S12

ABSTRACT Certain rpsL (which encodes the ribosomal protein S12) mutations that confer resistance to streptomycin markedly activate the production of antibiotics in Streptomyces spp. These rpsL mutations are known to be located in the two conserved regions within the S12 protein. To understand the roles of these two regions in the activation of silent genes, we used site-directed mutagenesis to generate eight novel mutations in addition to an already known (K88E) mutation that is capable of activating antibiotic production in Streptomyces lividans. Of these mutants, two (L90K and R94G) activated antibiotic production much more than the K88E mutant. Neither the L90K nor the R94G mutation conferred an increase in the level of resistance to streptomycin and paromomycin. Our results demonstrate the efficacy of the site-directed mutagenesis technique for strain improvement.

scholarly journals Saturation site-directed mutagenesis of thymidylate synthase.

1990 ◽  
Vol 265 (31) ◽  
pp. 18776-18779
Author(s):  
S Climie ◽  
L Ruiz-Perez ◽  
D Gonzalez-Pacanowska ◽  
P Prapunwattana ◽  
S.W. Cho ◽  
...  
Keyword(s):  
Thymidylate Synthase ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis

scholarly journals Probing Electrostatic Channeling in Protozoal Bifunctional Thymidylate Synthase-Dihydrofolate Reductase Using Site-directed Mutagenesis

2003 ◽  
Vol 278 (31) ◽  
pp. 28901-28911 ◽  
Author(s):  
Chloé E. Atreya ◽  
Eric F. Johnson ◽  
Jessica Williamson ◽  
Sing-Yang Chang ◽  
Po-Huang Liang ◽  
...  
Keyword(s):  
Dihydrofolate Reductase ◽  
Thymidylate Synthase ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis

scholarly journals Probing the Folate-binding Site of Human Thymidylate Synthase by Site-directed Mutagenesis

1998 ◽  
Vol 273 (47) ◽  
pp. 31209-31214 ◽  
Author(s):  
Youzhi Tong ◽  
Xinyue Liu-Chen ◽  
Emine A. Ercikan-Abali ◽  
Shi-Cheng Zhao ◽  
Debabrata Banerjee ◽  
...  
Keyword(s):  
Binding Site ◽  
Thymidylate Synthase ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis
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