Cell-Growth Regulation of the Hamster Dihydrofolate Reductase Gene Promoter by Transcription Factor Sp1

1997 ◽  
Vol 249 (1) ◽  
pp. 13-20 ◽  
Author(s):  
Veronique Noe ◽  
Chao Chen ◽  
Cristina Alemany ◽  
Marta Nicolas ◽  
Isabel Caragol ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Cell Growth ◽  
Dihydrofolate Reductase ◽  
Growth Regulation ◽  
Gene Promoter ◽  
Cell Growth Regulation ◽  
Transcription Factor Sp1 ◽  
Reductase Gene

The HIP1 binding site is required for growth regulation of the dihydrofolate reductase gene promoter

1992 ◽  
Vol 12 (3) ◽  
pp. 1054-1063
Author(s):  
A L Means ◽  
J E Slansky ◽  
S L McMahon ◽  
M W Knuth ◽  
P J Farnham
Keyword(s):  
Binding Site ◽  
Dihydrofolate Reductase ◽  
Growth Regulation ◽  
Gene Promoter ◽  
Nuclear Extract ◽  
Hela Nuclear Extract ◽  
Promoter Sequences ◽  
Reductase Gene ◽  
Protein Dna Interactions ◽  
Nih 3T3

The transcription rate of the dihydrofolate reductase (DHFR) gene increases at the G1/S boundary of the proliferative cell cycle. Through analysis of transiently and stably transfected NIH 3T3 cells, we have now demonstrated that DHFR promoter sequences extending from -270 to +20 are sufficient to confer similar regulation on a reporter gene. Mutation of a protein binding site that spans sequences from -16 to +11 in the DHFR promoter resulted in loss of the transcriptional increase at the G1/S boundary. Purification of an activity from HeLa nuclear extract that binds to this region enriched for a 180-kDa polypeptide (HIP1). Using this HIP1 preparation, we have identified specific positions within the binding site that are critical for efficient protein-DNA interactions. An analysis of association and dissociation rates suggests that bound HIP1 protein can exchange rapidly with free protein. This rapid exchange may facilitate the burst of transcriptional activity from the DHFR promoter at the G1/S boundary.

scholarly journals The HIP1 binding site is required for growth regulation of the dihydrofolate reductase gene promoter.

1992 ◽  
Vol 12 (3) ◽  
pp. 1054-1063 ◽  
Author(s):  
A L Means ◽  
J E Slansky ◽  
S L McMahon ◽  
M W Knuth ◽  
P J Farnham
Keyword(s):  
Binding Site ◽  
Dihydrofolate Reductase ◽  
Growth Regulation ◽  
Gene Promoter ◽  
Nuclear Extract ◽  
Hela Nuclear Extract ◽  
Promoter Sequences ◽  
Reductase Gene ◽  
Protein Dna Interactions ◽  
Nih 3T3

The transcription rate of the dihydrofolate reductase (DHFR) gene increases at the G1/S boundary of the proliferative cell cycle. Through analysis of transiently and stably transfected NIH 3T3 cells, we have now demonstrated that DHFR promoter sequences extending from -270 to +20 are sufficient to confer similar regulation on a reporter gene. Mutation of a protein binding site that spans sequences from -16 to +11 in the DHFR promoter resulted in loss of the transcriptional increase at the G1/S boundary. Purification of an activity from HeLa nuclear extract that binds to this region enriched for a 180-kDa polypeptide (HIP1). Using this HIP1 preparation, we have identified specific positions within the binding site that are critical for efficient protein-DNA interactions. An analysis of association and dissociation rates suggests that bound HIP1 protein can exchange rapidly with free protein. This rapid exchange may facilitate the burst of transcriptional activity from the DHFR promoter at the G1/S boundary.

MiR8181 is involved in the cell growth regulation of Saccharina japonica

2021 ◽  
pp. 153394
Author(s):  
Xiaoqi Yang ◽  
Xiuliang Wang ◽  
Jianting Yao ◽  
Wei Li ◽  
Delin Duan
Keyword(s):  
Cell Growth ◽  
Growth Regulation ◽  
Saccharina Japonica ◽  
Cell Growth Regulation

Transcription factor Sp1 recognizes a DNA sequence in the mouse dihydrofolate reductase promoter

Nature ◽  
1986 ◽  
Vol 319 (6050) ◽  
pp. 246-248 ◽  
Author(s):  
William S. Dynan ◽  
Shelley Sazer ◽  
Robert Tjian ◽  
Robert T. Schimke
Keyword(s):  
Transcription Factor ◽  
Dna Sequence ◽  
Dihydrofolate Reductase ◽  
Transcription Factor Sp1

Sphingolipid metabolism and cell growth regulation

1996 ◽  
Vol 10 (12) ◽  
pp. 1388-1397 ◽  
Author(s):  
Sarah Spiegel ◽  
Alfred H. Merrill
Keyword(s):  
Cell Growth ◽  
Growth Regulation ◽  
Sphingolipid Metabolism ◽  
Cell Growth Regulation
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