Trypanosoma cruzi: Biological Characterization of Clones Derived from Chronic Chagasic Patients. II. Quantitative Analysis of the Intracellular Cycle1

1985 ◽  
Vol 32 (1) ◽  
pp. 80-83 ◽  
Author(s):  
Juan C. Engel ◽  
Patricia S. Doyle ◽  
James A. Dvorak
Keyword(s):  
Quantitative Analysis ◽  
Trypanosoma Cruzi ◽  
Biological Characterization

scholarly journals Molecular and biological characterization of Trypanosoma cruzi strains isolated from children from Jequitinhonha Valley, State of Minas Gerais, Brazil

2013 ◽  
Vol 46 (4) ◽  
pp. 433-440 ◽  
Author(s):  
Jaquelline Carla Valamiel de Oliveira e Silva ◽  
Girley Francisco Machado de Assis ◽  
Maykon Tavares de Oliveira ◽  
Helder Magno Silva Valadares ◽  
Italo Faria do Valle ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Minas Gerais ◽  
Biological Characterization

scholarly journals Molecular characterization of Trypanosoma cruzi Mexican strains and their behavior in the mouse experimental model

2011 ◽  
Vol 44 (6) ◽  
pp. 684-690 ◽  
Author(s):  
César Gómez-Hernández ◽  
Karine Rezende-Oliveira ◽  
Gabriel Antônio Nogueira Nascentes ◽  
Lara Rocha Batista ◽  
Henrique Borges Kappel ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
Random Amplified Polymorphic Dna ◽  
Geographic Origin ◽  
Pcr Amplification ◽  
Biological Characterization ◽  
Long Time ◽  
Intergenic Regions ◽  
Mexican Strains

INTRODUCTION: For a long time, the importance of Chagas disease in Mexico, where many regarded it as an exotic malady, was questioned. Considering the great genetic diversity among isolates of Trypanosoma cruzi, the importance of this biological characterization, and the paucity of information on the clinical and biological aspects of Chagas disease in Mexico, this study aimed to identify the molecular and biological characterization of Trypanosoma cruzi isolates from different endemic areas of this country, especially of the State of Jalisco. METHODS: Eight Mexican Trypanosoma cruzi strains were biologically and genetically characterized (PCR specific for Trypanosoma cruzi, multiplex-PCR, amplification of space no transcript of the genes of the mini-exon, amplification of polymorphic regions of the mini-exon, classification by amplification of intergenic regions of the spliced leader genes, RAPD - (random amplified polymorphic DNA). RESULTS: Two profiles of parasitaemia were observed, patent (peak parasitaemia of 4.6×10(6) to 10(7) parasites/mL) and subpatent. In addition, all isolates were able to infect 100% of the animals. The isolates mainly displayed tropism for striated (cardiac and skeletal) muscle. PCR amplification of the mini-exon gene classified the eight strains as TcI. The RAPD technique revealed intraspecies variation among isolates, distinguishing strains isolated from humans and triatomines and according to geographic origin. CONCLUSIONS: The Mexican T. cruzi strains are myotrophic and belong to group TcI.

Biochemical, Immunological, and Biological Characterization of Trypanosoma cruzi Populations of the Andean North of Chile

1995 ◽  
Vol 81 (1) ◽  
pp. 125-135 ◽  
Author(s):  
J. Gonzalez ◽  
S. Munoz ◽  
S. Ortiz ◽  
D. Anacona ◽  
S. Salgado ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Biological Characterization

scholarly journals Biological characterization of Trypanosoma cruzi strains

2001 ◽  
Vol 96 (1) ◽  
pp. 53-59 ◽  
Author(s):  
Rafael A Martínez-Díaz ◽  
José A Escario ◽  
Juan J Nogal-Ruiz ◽  
Alicia Gómez-Barrio
Keyword(s):  
Trypanosoma Cruzi ◽  
Biological Characterization

scholarly journals Biological aspects of the DM28C clone of Trypanosoma cruzi after metacylogenesis in chemically defined media

1988 ◽  
Vol 83 (1) ◽  
pp. 123-133 ◽  
Author(s):  
Victor T. Contreras ◽  
Tania C. de Araújo-Jorge ◽  
Myrna C. Bonaldo ◽  
Neide Thomaz ◽  
Helena S. Barbosa ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Biological Properties ◽  
Biological Characterization ◽  
Phagocytic Cells ◽  
Biological Behaviour ◽  
Metacyclic Trypomastigotes ◽  
Bona Fide ◽  
Biological Aspects

The biological characterization of the Trypanosoma cruzi clone Dm 28c in terms of its growth in LIT medium, cell-cycle, infectivity to mice and interaction with professional and non-professional phagocytic cells shows that it behaves as a bona fide T. cruzi representant. The biological properties of this myotropic clone do not change according to the origin of the trypomastigote forms (i. e., from triatomines, infected mice, cell-culture or from the chemically defined TAUP and TAU3AAG media). In addition Dm 28c metacyclic trypomastigotes from TAU3AAG medium display a high infectivity level to fibroblasts and muscle cells. Experiments on binding of cationized ferritin to trypomastigotes surface show the existence of cap-like structures of ferritin in regions near the kinetoplast. However the nature and role of these anionic sites remain to be determined. The results indicate that metacyclic trypomastigotes from Dm 28c clone obtained under chemically defined conditions reproduce the biological behaviour of T. cruzi, rendering this system very suitable for the study of cell-parasite interactions and for the isolation of trypanosome relevant macromolecules.

Biological and molecular characterization of a Trypanosoma cruzi isolate obtained from Panstrongylus megistus captured in Sao Paulo State, Brazil

2014 ◽  
Vol 60 (1) ◽  
Author(s):  
Luciamáre P.A. Martins ◽  
Roberto E.P. Castanho ◽  
Altino L.S. Therezo ◽  
Aline R. Ribeiro ◽  
Luciana Lima ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Rflp Analysis ◽  
Sao Paulo ◽  
Prepatent Period ◽  
São Paulo ◽  
High Homology ◽  
Biological Characterization ◽  
Inherent Difficulty ◽  
Paulo State

AbstractAn isolate of Trypanosoma cruzi obtained from P. megistus captured in the peridomicile area of a home in Santo Antonio do Jardim city in the State of Sao Paulo, denominated T. cruzi Mogi, was characterized biologically and molecularly. The RFLP analysis of the D7 divergent domain in the 24Sα rDNA and of the mini-exon positioned the T. cruzi isolate within the TcI group. Phylogenetic analysis performed with the trypanosomatid barcode confirmed that the isolate belongs to the TcI group, with high homology to the 3014 c1 T.cruzi strain. The biological characterization of the isolate in rats showed a prepatent period of about 8 days, low parasitemia and tropism for cardiac, skeletal and colonic muscles. In Swiss mice the T. cruzi Mogi isolate showed a prepatent period of about 22 days, intermittent parasitemia in some animals, and tropism for cardiac and colonic muscles. Despite the inherent difficulty of identifying correlations amongst the molecular and biological characteristics of different T. cruzi groups, the tropism for colonic muscle demonstrated by T. cruzi Mogi represented a peculiarity of this isolate within the TcI group.

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