Sodium ion-coupled uptake of taurocholate by rat-liver plasma membrane vesicles

2008 ◽  
Vol 2 (1) ◽  
pp. 28-34 ◽  
Author(s):  
Peter G. Ruifrok ◽  
Dirk K. F. Meijer
Keyword(s):  
Plasma Membrane ◽  
Rat Liver ◽  
Membrane Vesicles ◽  
Sodium Ion ◽  
Plasma Membrane Vesicles ◽  
Liver Plasma Membrane

Thiamine transport by basolateral rat liver plasma membrane vesicles

1992 ◽  
Vol 103 (3) ◽  
pp. 1056-1065 ◽  
Author(s):  
Richard H. Moseley ◽  
Pankaj G. Vashi ◽  
Suzanne M. Jarose ◽  
Chris J. Dickinson ◽  
Patricia A. Permoad
Keyword(s):  
Plasma Membrane ◽  
Rat Liver ◽  
Membrane Vesicles ◽  
Plasma Membrane Vesicles ◽  
Liver Plasma Membrane ◽  
Thiamine Transport

scholarly journals Evidence for carrier-mediated chloride/bicarbonate exchange in canalicular rat liver plasma membrane vesicles.

1985 ◽  
Vol 75 (4) ◽  
pp. 1256-1263 ◽  
Author(s):  
P J Meier ◽  
R Knickelbein ◽  
R H Moseley ◽  
J W Dobbins ◽  
J L Boyer
Keyword(s):  
Plasma Membrane ◽  
Rat Liver ◽  
Membrane Vesicles ◽  
Plasma Membrane Vesicles ◽  
Liver Plasma Membrane

Multispecific anion exchange in basolateral (sinusoidal) rat liver plasma membrane vesicles

1986 ◽  
Vol 251 (5) ◽  
pp. G656-G664 ◽  
Author(s):  
G. Hugentobler ◽  
P. J. Meier
Keyword(s):  
Plasma Membrane ◽  
Rat Liver ◽  
Driving Forces ◽  
Competitive Inhibition ◽  
Membrane Vesicles ◽  
Ph Gradient ◽  
Disulfonic Acid ◽  
Sulfate Uptake ◽  
Plasma Membrane Vesicles ◽  
Liver Plasma Membrane

The mechanisms and driving forces for hepatic uptake of sulfate were investigated in basolateral (sinusoidal) rat liver plasma membrane vesicles. A transmembrane pH difference (pH 8.0 inside, 6.0 outside) stimulated sulfate uptake above equilibrium (“overshoot”). This pH gradient-stimulated sulfate uptake was saturable with increasing concentrations of sulfate and could be inhibited by probenecid, 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS), carbonyl cyanide p-(trifluoromethoxy)-phenylhydrazone, and nigericin. At low buffer concentrations and pH 6.0 an inwardly directed sodium gradient also stimulated sulfate uptake. This sodium-dependent sulfate uptake could be inhibited by amiloride and DIDS, indicating indirect coupling of sodium and sulfate flux through concomitant sodium-proton and sulfate-hydroxyl exchange. Cisinhibition of initial pH gradient-stimulated sulfate uptake, as well as transstimulation of sulfate uptake under pH-equilibrated conditions (pH 7.5 inside and outside), were observed with sulfate, thiosulfate, oxalate, and succinate, but not with chloride, bicarbonate, acetate, lactate, pyruvate, p-aminohippurate, citrate, glutamate, aspartate, and taurocholate. Furthermore, cholate and sulfobromophthalein exhibited competitive inhibition of pH gradient-stimulated sulfate uptake. In addition, an inside-to-outside hydroxyl gradient also stimulated uptake of cholate and this pH gradient-sensitive portion of cholate uptake was inhibited by extravesicular sulfate. In contrast to basolateral membranes, no evidence for multispecific sulfate-hydroxyl exchange was found in canalicular plasma membrane vesicles.

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