scholarly journals Chromosomal Localization of the Major Histocompatibility Complex in Cattle and River Buffalo by Fluorescent in Situ Hybridization

Hereditas ◽  
2004 ◽  
Vol 118 (2) ◽  
pp. 187-190 ◽  
Author(s):  
L. Iannuzzi ◽  
D. S. Gallagher ◽  
J. E. Womack ◽  
G. P. Di Meo ◽  
L. C. Skow ◽  
...  
Keyword(s):  
Major Histocompatibility Complex ◽  
In Situ Hybridization ◽  
Fluorescent In Situ Hybridization ◽  
Chromosomal Localization ◽  
River Buffalo ◽  
Major Histocompatibility ◽  
Histocompatibility Complex

scholarly journals Molecular analysis of mouse spermatogenesis: isolation of the t-complex polypeptide-1 gene and related sequences

Development ◽  
1986 ◽  
Vol 97 (Supplement) ◽  
pp. 151-156
Author(s):  
Keith Willison ◽  
Keith Dudley ◽  
Jean Potter ◽  
Rebecca Haffner ◽  
Christine Watson
Keyword(s):  
Major Histocompatibility Complex ◽  
In Situ Hybridization ◽  
Molecular Analysis ◽  
Wild Mouse ◽  
Chromosome 17 ◽  
Major Histocompatibility ◽  
T Complex ◽  
Histocompatibility Complex ◽  
Normal Laboratory

The mouse t-complex is a region of chromosome 17, found in wild mouse populations, which is grossly rearranged when compared to those of normal laboratory strains. So far, two large, independent inversions have been demonstrated. The distal inversion includes the entire Major Histocompatibility Complex (MHC) (Artzt, Shin & Bennett, 1982; Shin et al. 1983; Pla & Condamine, 1984) and the recently discovered proximal inversion (Herrmann et al. 1986) also contains many genes, including the t-complex polypeptide-1 gene (Tcp-1) discussed in this article. Using in situ hybridization, the MHC (Lader et al. 1985) and Tcp-1 (Lyon et al. 1986) genes have been positioned on chromosome 17 and the t-complex would appear to occupy Giemsa bands 17B and 17A3, representing roughly 15 % of the chromosome. Presumably, in addition to those already mapped, many hundreds of genes are located in this region.

scholarly journals Localization of major histocompatibility complex class I and II mRNA in human first-trimester chorionic villi by in situ hybridization.

1992 ◽  
Vol 175 (4) ◽  
pp. 1027-1032 ◽  
Author(s):  
J A Lata ◽  
R S Tuan ◽  
K J Shepley ◽  
M M Mulligan ◽  
L G Jackson ◽  
...  
Keyword(s):  
Major Histocompatibility Complex ◽  
In Situ Hybridization ◽  
Chorionic Villus ◽  
First Trimester ◽  
Class I ◽  
Immune Recognition ◽  
Major Histocompatibility ◽  
Histocompatibility Complex ◽  
Villous Trophoblast

Maternal immune recognition of pregnancy occurs despite the nonexpression of classical major histocompatibility complex (MHC) antigenic determinants by chorionic villous trophoblast, which comprise the major surface area where maternal blood contacts fetal-derived cells. cDNA-mRNA in situ hybridization was used to probe expression of transcripts corresponding to nonpolymorphic MHC determinants in first-trimester chorionic villus samples. The HLA-B7 probe hybridization signals were localized to syncytiotrophoblast and to cells of the mesenchyme but not to villous cytotrophoblast. HLA-G mRNA was found only in syncytiotrophoblast. A DR beta clone hybridized to both villous cytotrophoblast and syncytiotrophoblast. The results suggest that expression of trophoblast class I and class II determinants early in gestation (10 wk) may be regulated by posttranscriptional events. This also suggests the potential for maternal antifetal alloimmune responses.

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