Relative efficiency of maize andImperata cylindricafor haploid induction inTriticum durumfollowing chromosome elimination-mediated approach of doubled haploid breeding

Doubled haploid technology is widely applied in maize. The haploid inducer lines play critical roles in doubled haploid breeding. We report the development of specialized haploid inducer lines that enhance the purple pigmentation of crossing immature embryos. During the development of haploid inducer lines, two breeding populations derived from the CAU3/S23 and CAU5/S23 were used. Molecular marker-assisted selection for both qhir1 and qhir8 was used from BC1F1 to BC1F4. Evaluation of the candidate individuals in each generation was carried out by pollinating to the tester of ZD958. Individuals with fast and clear pigmentation of the crossing immature embryos, high number of haploids per ear, and high haploid induction rate were considered as candidates. Finally, three new haploid inducer lines (CS1, CS2, and CS3) were developed. The first two (CS1 and CS2) were from the CAU3/S23, with a haploid induction rate of 8.29%–13.25% and 11.54%–15.54%, respectively. Meanwhile, the CS3 was from the CAU5/S23. Its haploid induction rate was 8.14%–12.28%. In comparison with the donor haploid inducer lines, the 24-h purple embryo rates of the newly developed haploid inducer lines were improved by 10%–20%, with a ~90% accuracy for the identification of haploid immature embryos. These new haploid inducer lines will further improve the efficiency of doubled haploid breeding of maize.

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Influence of different wheat and Imperata cylindrica genetic backgrounds on haploid induction efficiency in wheat doubled haploid breeding

Czech Journal of Genetics and Plant Breeding ◽

10.17221/105/2013-cjgpb ◽

2014 ◽

Vol 50 (No. 3) ◽

pp. 195-200 ◽

Cited By ~ 2

Author(s):

S.A. Rather ◽

H.K. Chaudhary ◽

V. Kaila

Keyword(s):

Bread Wheat ◽

Doubled Haploid ◽

Combining Ability ◽

General Combining Ability ◽

Imperata Cylindrica ◽

Haploid Induction ◽

Embryo Formation ◽

Haploid Production ◽

Formation Efficiency ◽

Doubled Haploid Breeding

Four Indian and one Japanese accession of Imperata cylindrica were assessed for their influence upon haploid production in F<sub>1</sub> generations of 21 wheat crosses (winter × spring, spring × spring and winter × winter) to find an efficient pollen source for haploid induction, which would enhance doubled haploid breeding in bread wheat. The frequency of haploid induction was influenced differently by the wheat and the I. cylindrica genotypes, indicating both maternal and paternal genetic influence on haploid induction. The gene actions controlling the inheritance of  haploid induction  appeared to be non-additive. Haploid formation efficiency was closely associated with other haploid induction parameters, i.e. pseudoseed formation, embryo formation and haploid regeneration. Amongst wheat F<sub>1</sub> groups, spring × spring wheats exhibited the highest potential for haploid induction. General combining ability for haploid production was highest for the, I. cylindrica genotype Ic-Aru, native to the northeastern Himalayas, which appears as a potential pollen source for efficient haploid induction in bread wheat.

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Relative efficiency of different Gramineae genera for haploid induction in triticale and triticale x wheat hybrids through the chromosome elimination technique

Plant Breeding ◽

10.1111/j.1439-0523.2004.01059.x ◽

2005 ◽

Vol 124 (2) ◽

pp. 147-153 ◽

Cited By ~ 29

Author(s):

A. Pratap ◽

G. S. Sethi ◽

H. K. Chaudhary

Keyword(s):

Relative Efficiency ◽

Chromosome Elimination ◽

Haploid Induction ◽

Wheat Hybrids

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Doubled haploid production in maize under Sub-montane Himalayan conditions using R1-nj-based haploid inducer TAILP1

Indian Journal of Genetics and Plant Breeding (The) ◽

10.31742/ijgpb.80.3.4 ◽

2020 ◽

Vol 80 (03) ◽

Author(s):

R. K Khulbe ◽

A. Pattanayak ◽

Lakshmi Kant ◽

G. S. Bisht ◽

M. C. Pant ◽

...

Keyword(s):

Doubled Haploid ◽

Sweet Corn ◽

Haploid Induction ◽

Maize Breeding ◽

Line Production ◽

Haploid Inducer ◽

Breeding Programmes ◽

Source Populations ◽

Developmental Phases

The use of in vivo haploid induction system makes the doubled haploid (DH) technology easier to adopt for the conventional maize breeders. However, despite having played an important role in the initial developmental phases of DH technology, Indian maize research has yet to harvest its benefits. Haploid Inducer Lines (HILs) developed by CIMMYT are being widely used in maize breeding programmes in many countries including India. There, however, is no published information on the efficiency of DH line production using CIMMYT HILs in Indian maize breeding programmes. In the present study, the efficiency of DH production using CIMMYT’s tropically adapted inducer line TAILP1 was investigated with eight source populations including two of sweet corn. The average haploid induction rate (HIR) of TAILP1 was 5.48% with a range of 2.01 to 10.03%. Efficiency of DH production ranged from 0.14 to 1.87% for different source populations with an average of 1.07%. The information generated will be useful for maize breeders intending to use DH technology for accelerated development of completely homozygous lines.

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Doubled haploid technology for line development in maize: technical advances and prospects

Theoretical and Applied Genetics ◽

10.1007/s00122-019-03433-x ◽

2019 ◽

Vol 132 (12) ◽

pp. 3227-3243 ◽

Cited By ~ 14

Author(s):

Vijay Chaikam ◽

Willem Molenaar ◽

Albrecht E. Melchinger ◽

Prasanna M. Boddupalli

Keyword(s):

Doubled Haploid ◽

Chromosome Doubling ◽

Production Costs ◽

Great Promise ◽

Success Rates ◽

Haploid Induction ◽

Maize Breeding ◽

Breeding Programs ◽

Line Production

Key Message Increased efficiencies achieved in different steps of DH line production offer greater benefits to maize breeding programs. Abstract Doubled haploid (DH) technology has become an integral part of many commercial maize breeding programs as DH lines offer several economic, logistic and genetic benefits over conventional inbred lines. Further, new advances in DH technology continue to improve the efficiency of DH line development and fuel its increased adoption in breeding programs worldwide. The established method for maize DH production covered in this review involves in vivo induction of maternal haploids by a male haploid inducer genotype, identification of haploids from diploids at the seed or seedling stage, chromosome doubling of haploid (D0) seedlings and finally, selfing of fertile D0 plants. Development of haploid inducers with high haploid induction rates and adaptation to different target environments have facilitated increased adoption of DH technology in the tropics. New marker systems for haploid identification, such as the red root marker and high oil marker, are being increasingly integrated into new haploid inducers and have the potential to make DH technology accessible in germplasm such as some Flint, landrace, or tropical material, where the standard R1-nj marker is inhibited. Automation holds great promise to further reduce the cost and time in haploid identification. Increasing success rates in chromosome doubling protocols and/or reducing environmental and human toxicity of chromosome doubling protocols, including research on genetic improvement in spontaneous chromosome doubling, have the potential to greatly reduce the production costs per DH line.

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