scholarly journals Molecular characterization of variant infectious bronchitis virus in China, 2019: Implications for control programmes

2020 ◽  
Vol 67 (3) ◽  
pp. 1349-1355 ◽  
Author(s):  
Xiaozhan Zhang ◽  
Tongwei Deng ◽  
Jianzhou Lu ◽  
Pandeng Zhao ◽  
Lulu Chen ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Infectious Bronchitis Virus ◽  
Infectious Bronchitis ◽  
Control Programmes

Isolation and Molecular Characterization of Novel Infectious Bronchitis Virus Variants from Vaccinated Broiler Flocks in Egypt

2017 ◽  
Vol 61 (3) ◽  
pp. 307-310 ◽  
Author(s):  
Mohammed A. Abdel-Sabour ◽  
Emad M. Al-Ebshahy ◽  
Samy A. Khaliel ◽  
Nabil A. Abdel-Wanis ◽  
Tokuma Yanai
Keyword(s):  
Molecular Characterization ◽  
Infectious Bronchitis Virus ◽  
Infectious Bronchitis

scholarly journals Prevalence and molecular characterization of infectious bronchitis virus isolated from chicken in Bangladesh

2019 ◽  
Vol 12 (6) ◽  
pp. 909-915 ◽  
Author(s):  
Zafar Ahmed Bhuiyan ◽  
Md. Zulfekar Ali ◽  
Mohammad Moktader Moula ◽  
Md. Giasuddin ◽  
Zahed Uddin Mahmood Khan
Keyword(s):  
Molecular Characterization ◽  
Infectious Bronchitis Virus ◽  
Allantoic Fluid ◽  
Rt Pcr ◽  
Broiler Breeder ◽  
Infectious Bronchitis ◽  
Chicken Egg ◽  
Chicken Eggs ◽  
Allantoic Cavity

Aim: The present study was aimed to determine the prevalence of infectious bronchitis virus (IBV) as well as virus isolation, identification, and molecular characterization of various strains circulating in Bangladesh. Materials and Methods: A total of 371 swabs and organ samples were collected from four types of chicken including layer, Sonali (local), broiler, and broiler breeder under eight districts (Rangpur, Bogura, Tangail, Dhaka, Gazipur, Mymensingh, Jamalpur, and Cumilla) during 2014-2016 in Bangladesh. Results: Out of 371 samples, 65 samples were positive in reverse transcriptase polymerase chain reaction (RT-PCR) for molecular identification of IBV. The overall prevalence was 17.52% recorded and among the selected types of chicken, the highest prevalence of IBV was found in layer that was 42.22% followed by 17.24% in Sonali, 14.93% in broiler breeder, and lowest prevalence was 11.94% in broiler chicken, respectively. Moreover, the prevalence of IBV was recorded highest in aged chicken at 41-60 weeks, which was 54.55% in layer, 27.27% in Sonali, and, afterward, 14.68% was found in broiler breeder, respectively. Frequency of IBV more frequently in winter (22.67%) followed by rainy (15.87%) and summer season (11.58%). The highest prevalence of IBV was found Tangail district (41.67%) followed by Mymensingh (24.42%), Gazipur (19.32%), Dhaka (15.38%), Jamalpur (16.67%), Bogura (13.68%), Cumilla (5.88%), and Rangpur (9.26%), respectively. Samples that were found high positive in IBV RT-PCR (Ct value below 30) were subjected to inoculation into chicken egg embryo to observe characteristic changes in chicken embryo. Swabs and organ samples were processed and passaged in 9-day-old embryonated chicken eggs through allantoic cavity route. IBV virus suspected samples inoculated into chicken egg embryos after 3-5 passages showed dwarfing and curling of the embryos which are characteristic lesions of IBV. Allantoic fluid was collected from all inoculated eggs and performed partial sequencing of S1 gene for three isolates. After sequencing, the phylogenetic tree was constructed from the nucleotide sequences of IBV isolates. Two of the isolates are 4/91 IBV and another one matched with QX-like IBV. Conclusion: The results revealed that the three isolates from different places in Bangladesh were identified for the 1st time as which will help for IBV control strategy.

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