scholarly journals Caveats of HLA antibody detection by solid‐phase assays

2019 ◽  
Vol 33 (1) ◽  
pp. 18-29 ◽  
Author(s):  
Caroline Wehmeier ◽  
Gideon Hönger ◽  
Stefan Schaub
Keyword(s):  
Solid Phase ◽  
Antibody Detection ◽  
Hla Antibody

HLA Antibody Detection and Characterization by Solid Phase Immunoassays: Methods and Pitfalls

2012 ◽  
pp. 289-308 ◽  
Author(s):  
Andrea A. Zachary ◽  
Renato M. Vega ◽  
Donna P. Lucas ◽  
Mary S. Leffell
Keyword(s):  
Solid Phase ◽  
Antibody Detection ◽  
Hla Antibody

7-P: Inter-laboratory comparison of HLA antibody detection by solid phase methods

2009 ◽  
Vol 70 ◽  
pp. S15
Author(s):  
Deborah O. Crowe ◽  
Sallyanne C. Fossey
Keyword(s):  
Solid Phase ◽  
Antibody Detection ◽  
Hla Antibody

Solid phase HLA antibody detection technology - challenges in interpretation

2010 ◽  
Author(s):  
B. D. Tait ◽  
F. Hudson ◽  
G. Brewin ◽  
L. Cantwell ◽  
R. Holdsworth
Keyword(s):  
Solid Phase ◽  
Antibody Detection ◽  
Detection Technology ◽  
Hla Antibody

scholarly journals To Evaluate Anti-HLA Antibodies Sensitization in Pre- and Post-renal Transplant Patient’s Serum: A Retrospective Case Series

2019 ◽  
pp. e000187
Author(s):  
Nishtha Agarwal ◽  
Sanjeev Goswami
Keyword(s):  
Acute Rejection ◽  
De Novo ◽  
Solid Phase ◽  
Case Series ◽  
Antibody Detection ◽  
Unrelated Donor ◽  
Retrospective Case ◽  
Post Transplant ◽  
Financial Condition ◽  
Hla Antibody

Introduction: In India, patients of renal failure are dependent on live related or unrelated donor. Because of poor financial condition patients do not go for DSA (Donor Specific Antibody) detection using Luminex. In absence of screening of de-novo production of DSA and don’t get proper management. As a result of which patient undergo acute rejection. Case Series: Here we are presenting 5 acute rejection cases comparing there DSA in pre-transplant and post-transplant sera using solid-phase assays.5 renal transplanted patients undergone acute and hyperacute rejection (Banff's classification) were considered for presented case series. Collected serum (pre and post-transplant on day of rejection) from each patient was subjected to detection of anti-HLA antibody using Luminex-PRA. Conclusion: Presence of donor-specific anti-HLA antibody with their titer was detected in pre and post-transplant serum. It is found that the strength of DSA is directly proportional to graft life.

Solid-phase HLA antibody detection methods and risk of renal allograft rejection in children

2001 ◽  
Vol 33 (1-2) ◽  
pp. 403-404 ◽  
Author(s):  
B.K Book ◽  
P Agrawal ◽  
L.K Dalla Vecchia ◽  
H.M Gebel ◽  
M.D Pescovitz ◽  
...  
Keyword(s):  
Allograft Rejection ◽  
Renal Allograft ◽  
Solid Phase ◽  
Detection Methods ◽  
Antibody Detection ◽  
Renal Allograft Rejection ◽  
Hla Antibody

Role of crossmatch testing when Luminex-SAB is negative in renal transplantation

2018 ◽  
Vol 90 (1) ◽  
pp. 41-46 ◽  
Author(s):  
Kumar Jayant ◽  
Isabella Reccia ◽  
Bridson M Julie ◽  
Ajay Sharma ◽  
Ahmed Halawa
Keyword(s):  
Solid Phase ◽  
Human Leukocyte ◽  
Hla Typing ◽  
Antibody Detection ◽  
Renal Transplant Recipients ◽  
Transplant Recipients ◽  
Detection Techniques ◽  
Leukocyte Antigen ◽  
Hla Antibody

The human leukocyte antigen (HLA) system plays an important role in the acceptance of renal graft. Long and better graft survival has been reported in patients with HLA-identical siblings and a nonreactive cytotoxicity assay (CDC). New methods of HLA-typing and anti-HLA antibody detection techniques such as flow cytometry, solid-phase immunoassays, or antigen bead assays have further improved the outcomes of renal transplant recipients. In the present review, the explicit details of these methodologies are discussed in detail.

Concordance with established tests and reproducibility of results obtained with a new single antigen chip array for HLA antibody detection (HISTO SPOT® HLA AB)

2021 ◽  
Vol 491 ◽  
pp. 112971
Author(s):  
Kristin Launhardt ◽  
Virginie Lefevre ◽  
Vianney Souplet ◽  
Livia Prantl ◽  
Matthias Marget ◽  
...  
Keyword(s):  
Reproducibility Of Results ◽  
Antibody Detection ◽  
Single Antigen ◽  
Hla Antibody

65-P: Solid phase anti-HLA antibody testing: Is a low level of antibody clinically relevant?

2007 ◽  
Vol 68 (1) ◽  
pp. S47
Author(s):  
Allen J. Norin ◽  
Ballabh Das ◽  
David Hochman ◽  
Andrew D’Onofrio ◽  
Louise Bergamini ◽  
...  
Keyword(s):  
Solid Phase ◽  
Antibody Testing ◽  
Low Level ◽  
Hla Antibody
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