scholarly journals Calcium-activated potassium channels in isolated presynaptic nerve terminals from rat brain.

1985 ◽  
Vol 361 (1) ◽  
pp. 441-457 ◽  
Author(s):  
D K Bartschat ◽  
M P Blaustein
Keyword(s):  
Potassium Channels ◽  
Rat Brain ◽  
Nerve Terminals ◽  
Calcium Activated Potassium Channels ◽  
Presynaptic Nerve Terminals

scholarly journals Potassium channels in isolated presynaptic nerve terminals from rat brain.

1985 ◽  
Vol 361 (1) ◽  
pp. 419-440 ◽  
Author(s):  
D K Bartschat ◽  
M P Blaustein
Keyword(s):  
Potassium Channels ◽  
Rat Brain ◽  
Nerve Terminals ◽  
Presynaptic Nerve Terminals

scholarly journals Modulation of calcium-activated potassium channels from rat brain by protein kinase A and phosphatase 2A

1991 ◽  
Vol 11 (6) ◽  
pp. 1627-1635 ◽  
Author(s):  
PH Reinhart ◽  
S Chung ◽  
BL Martin ◽  
DL Brautigan ◽  
IB Levitan
Keyword(s):  
Protein Kinase ◽  
Potassium Channels ◽  
Protein Kinase A ◽  
Rat Brain ◽  
Phosphatase 2A ◽  
Calcium Activated Potassium Channels ◽  
Kinase A

Development of ?-Noradrenergic and Dopaminergic Receptor Systems Depends on Maturation of Their Presynaptic Nerve Terminals in the Rat Brain

1981 ◽  
Vol 36 (5) ◽  
pp. 1683-1690 ◽  
Author(s):  
R. Deskin ◽  
F. J. Seidler ◽  
W. L. Whitmore ◽  
T. A. Slotkin
Keyword(s):  
Rat Brain ◽  
Nerve Terminals ◽  
Dopaminergic Receptor ◽  
Presynaptic Nerve Terminals

scholarly journals The regulation of cytosolic pH in isolated presynaptic nerve terminals from rat brain.

1988 ◽  
Vol 91 (2) ◽  
pp. 289-303 ◽  
Author(s):  
D A Nachshen ◽  
P Drapeau
Keyword(s):  
Rat Brain ◽  
Parent Compound ◽  
Mammalian Brain ◽  
Fluorescence Signal ◽  
Nerve Terminals ◽  
Free Medium ◽  
Cytosolic Ph ◽  
Methyl Ester Derivative ◽  
Synaptosomal Membrane ◽  
Presynaptic Nerve Terminals

Cytosolic pH (pHi) was measured in presynaptic nerve terminals isolated from rat brain (synaptosomes) using a fluorescent pH indicator, 2',7'-bis(carboxyethyl)-5,6-carboxyfluorescein (BCECF). The synaptosomes were loaded with BCECF by incubation with the membrane-permanent acetoxy-methyl ester derivative of BCECF, which is hydrolyzed by intracellular esterases to the parent compound. pHi was estimated by calibrating the fluorescence signal after permeabilizing the synaptosomal membrane by two different methods. Synaptosomes loaded with 15-90 microM BCECF were estimated to have a pHi of 6.94 +/- 0.02 (mean +/- standard error; n = 54) if the fluorescence signal was calibrated after permeabilizing with digitonin; a similar value was obtained using synaptosomes loaded with 10 times less BCECF (6.9 +/- 0.1; n = 5). When the fluorescence signal was calibrated by permeabilizing the synaptosomal membrane to H+ with gramicidin and nigericin, pHi was estimated to be 7.19 +/- 0.03 (n = 12). With the latter method, pHi = 6.95 +/- 0.09 (n = 14) when the synaptosomes were loaded with 10 times less BCECF. Thus, pHi in synaptosomes was approximately 7.0 and could be more precisely monitored using the digitonin calibration method at higher BCECF concentrations. When synaptosomes were incubated in medium containing 20 mM NH4Cl and then diluted into NH4Cl-free medium, pHi immediately acidified to a level of approximately 6.6. After the acidification, pHi recovered over a period of a few minutes. The buffering capacity of the synaptosomes was estimated to be approximately 50 mM/pH unit. Recovery was substantially slowed by incubation in an Na-free medium, by the addition of amiloride (KI = 3 microM), and by abolition of the Nao/Nai gradient. pHi and its recovery after acidification were not affected by incubation in an HCO3-containing medium; disulfonic stilbene anion transport inhibitors (SITS and DIDS, 1 mM) and replacement of Cl with methylsulfonate did not affect the rate of recovery of pHi. It appears that an Na+/H+ antiporter is the primary regulator of pHi in mammalian brain nerve terminals.

scholarly journals Syntaxin 1A Co‐Associates with Native Rat Brain and Cloned Large Conductance, Calcium‐Activated Potassium Channels in situ

2003 ◽  
Vol 553 (1) ◽  
pp. 65-81 ◽  
Author(s):  
Shizhang Ling ◽  
Jian‐Zhong Sheng ◽  
Janice E. A. Braun ◽  
Andrew P. Braun
Keyword(s):  
Potassium Channels ◽  
Rat Brain ◽  
Syntaxin 1A ◽  
Calcium Activated Potassium Channels

Regulation of Cytosolic pH in Presynaptic Nerve Terminals Isolated from Rat Brain

1987 ◽  
Vol 494 (1 Third Colloqu) ◽  
pp. 240-242
Author(s):  
DANIEL A. NACHSHEN
Keyword(s):  
Rat Brain ◽  
Nerve Terminals ◽  
Cytosolic Ph ◽  
Presynaptic Nerve Terminals

scholarly journals Dual Effects of Nitric Oxide on the Large Conductance Calcium-activated Potassium Channels of Rat Brain

BMB Reports ◽  
2006 ◽  
Vol 39 (1) ◽  
pp. 91-96 ◽  
Author(s):  
Ji-Eun Lee ◽  
Ji-Yeon Kwak ◽  
Chang-Kook Suh ◽  
Jung-Hoon Shin
Keyword(s):  
Nitric Oxide ◽  
Potassium Channels ◽  
Rat Brain ◽  
Calcium Activated Potassium Channels

Binary mixtures of pyrethroids produce differential effects on Ca2+ influx and glutamate release at isolated presynaptic nerve terminals from rat brain

2011 ◽  
Vol 99 (2) ◽  
pp. 131-139 ◽  
Author(s):  
Steven B. Symington ◽  
Hilliary E. Hodgdon ◽  
Richard K. Frisbie ◽  
J. Marshall Clark
Keyword(s):  
Rat Brain ◽  
Binary Mixtures ◽  
Glutamate Release ◽  
Nerve Terminals ◽  
Differential Effects ◽  
Presynaptic Nerve Terminals
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