Density-Gradient Centrifugation with Infectious Ribonucleic Acid of Foot-and-Mouth Disease Virus

Science ◽  
1959 ◽  
Vol 130 (3369) ◽  
pp. 217-217 ◽  
Author(s):  
K. STROHMAIER ◽  
M. MUSSGAY
Keyword(s):  
Density Gradient ◽  
Disease Virus ◽  
Ribonucleic Acid ◽  
Density Gradient Centrifugation ◽  
Gradient Centrifugation ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Mouth Disease Virus ◽  
Foot And Mouth

Foot and mouth disease virus I. Stability of its ribonucleic acid

Virology ◽  
1978 ◽  
Vol 84 (1) ◽  
pp. 230-235 ◽  
Author(s):  
Claudio D. Denoya ◽  
Eduardo A. Scodeller ◽  
Beatriz H. Gimenez ◽  
César Vásquez ◽  
JoséL. La Torre
Keyword(s):  
Disease Virus ◽  
Ribonucleic Acid ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Mouth Disease Virus ◽  
Foot And Mouth

Highly Purified Foot-and-Mouth Disease Virus: Optical and Biological Measurements during Zone Electrophoresis in a Glucose Density Gradient

1966 ◽  
Vol 21 (8) ◽  
pp. 774-782 ◽  
Author(s):  
H. D. Matheka ◽  
H. L. Bachrach ◽  
R. Trautman
Keyword(s):  
Density Gradient ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Optical Methods ◽  
High Passage ◽  
Free Zone ◽  
Mouth Disease Virus ◽  
Zone Electrophoresis ◽  
Foot And Mouth

Highly purified, concentrated foot-and-mouth disease virus, type A, was homogeneous and monodisperse by carrier-free zone electrophoresis in a glucose density gradient. Virus mobilities in veronal-acetate buffer, pH 8.6 and ionic strength 0.1, were identical whether determined by infectivity or optical methods. Low passage calf-kidney virus had a significantly higher mobility, 3.28 × 10-5 cm2/volt · sec, than high passage virus, 2.84 × 10-5 cm2/volt · sec. Single passages of the high passage virus in baby hamster kidney cells did not change its mobility. Regardless of passage history, storage at 4°C in 0.05 M phosphate at pH 7.5 increased mobilities to about 3.75 × 10-5 cm2/volt · sec with retention of infectivity; some virions broke down to protein and ribonucleic acid. These electrophoretically distinct viruses possessed different absorbance-temperature profiles but identical sedimentation constants.

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