scholarly journals Erratum for Liu et al., “Ethanol Metabolism Dynamics in Clostridium ljungdahlii Grown on Carbon Monoxide”

2020 ◽  
Vol 86 (23) ◽  
Author(s):  
Zi-Yong Liu ◽  
De-Chen Jia ◽  
Kun-Di Zhang ◽  
Hai-Feng Zhu ◽  
Quan Zhang ◽  
...  
Keyword(s):  
Carbon Monoxide ◽  
Ethanol Metabolism ◽  
Clostridium Ljungdahlii

scholarly journals Ethanol Metabolism Dynamics in Clostridium ljungdahlii Grown on Carbon Monoxide

2020 ◽  
Vol 86 (14) ◽  
Author(s):  
Zi-Yong Liu ◽  
De-Chen Jia ◽  
Kun-Di Zhang ◽  
Hai-Feng Zhu ◽  
Quan Zhang ◽  
...  
Keyword(s):  
Carbon Monoxide ◽  
Ethanol Production ◽  
Biomass Production ◽  
Ethanol Oxidation ◽  
Ethanol Yield ◽  
Ethanol Metabolism ◽  
Content Type ◽  
Atp Production ◽  
Clostridium Ljungdahlii ◽  
Gas Fermentation

ABSTRACT Bioethanol production from syngas using acetogenic bacteria has attracted considerable attention in recent years. However, low ethanol yield is the biggest challenge that prevents the commercialization of syngas fermentation into biofuels using microbial catalysts. The present study demonstrated that ethanol metabolism plays an important role in recycling NADH/NAD+ during autotrophic growth. Deletion of bifunctional aldehyde/alcohol dehydrogenase (adhE) genes leads to significant growth deficiencies in gas fermentation. Using specific fermentation technology in which the gas pressure and pH were constantly controlled at 0.1 MPa and 6.0, respectively, we revealed that ethanol was formed during the exponential phase, closely accompanied by biomass production. Then, ethanol was oxidized to acetate via the aldehyde ferredoxin oxidoreductase pathway in Clostridium ljungdahlii. A metabolic experiment using 13C-labeled ethanol and acetate, redox balance analysis, and comparative transcriptomic analysis demonstrated that ethanol production and reuse shared the metabolic pathway but occurred at different growth phases. IMPORTANCE Ethanol production from carbon monoxide (CO) as a carbon and energy source by Clostridium ljungdahlii and “Clostridium autoethanogenum” is currently being commercialized. During gas fermentation, ethanol synthesis is NADH-dependent. However, ethanol oxidation and its regulatory mechanism remain incompletely understood. Energy metabolism analysis demonstrated that reduced ferredoxin is the sole source of NADH formation by the Rnf-ATPase system, which provides ATP for cell growth during CO fermentation. Therefore, ethanol production is tightly linked to biomass production (ATP production). Clarification of the mechanism of ethanol oxidation and biosynthesis can provide an important reference for generating high-ethanol-yield strains of C. ljungdahlii in the future.

scholarly journals Directing Clostridium ljungdahlii fermentation products via hydrogen to carbon monoxide ratio in syngas

2019 ◽  
Vol 124 ◽  
pp. 95-101 ◽  
Author(s):  
Joshua Jack ◽  
Jonathan Lo ◽  
Pin-Ching Maness ◽  
Zhiyong Jason Ren
Keyword(s):  
Carbon Monoxide ◽  
Fermentation Products ◽  
Clostridium Ljungdahlii

Tracing carbon monoxide uptake by Clostridium ljungdahlii during ethanol fermentation using 13C-enrichment technique

2012 ◽  
Vol 36 (5) ◽  
pp. 591-595 ◽  
Author(s):  
Seok-In Yun ◽  
Seong-Joo Gang ◽  
Hee-Myong Ro ◽  
Min-Jin Lee ◽  
Woo-Jung Choi ◽  
...  
Keyword(s):  
Carbon Monoxide ◽  
Ethanol Fermentation ◽  
Clostridium Ljungdahlii ◽  
Enrichment Technique

Carbon monoxide poisoning

2000 ◽  
Vol 12 (4) ◽  
pp. 354-357
Author(s):  
David R Smart ◽  
Paul D Mark
Keyword(s):  
Carbon Monoxide ◽  
Carbon Monoxide Poisoning

Transformation of Cooperative Free Energies between Ligation Systems of Hemoglobin:  Resolution of the Carbon Monoxide Binding Intermediates†

Biochemistry ◽  
1996 ◽  
Vol 35 (3) ◽  
pp. 704-718 ◽  
Author(s):  
Yingwen Huang ◽  
Gary K. Ackers
Keyword(s):  
Carbon Monoxide ◽  
Free Energies ◽  
Carbon Monoxide Binding

EPA Retains Limits On Carbon Monoxide

2011 ◽  
pp. 012511122338
Author(s):  
Glenn Hess
Keyword(s):  
Carbon Monoxide

Ethanol metabolism in suspension cultured carrot cells

1991 ◽  
Vol 82 (1) ◽  
pp. 103-108
Author(s):  
P. Perata ◽  
A. Alpi
Keyword(s):  
Ethanol Metabolism ◽  
Carrot Cells

Carbon monoxide in the home

2003 ◽  
Keyword(s):  
Carbon Monoxide

Deep-diving seals reveal secrets of carbon monoxide

Nature ◽  
2014 ◽  
Author(s):  
Jessica Marshall
Keyword(s):  
Carbon Monoxide ◽  
Deep Diving

Dangers of Carbon Monoxide

1889 ◽  
Vol 27 (703supp) ◽  
pp. 11238-11238
Keyword(s):  
Carbon Monoxide
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