scholarly journals Rapid Fluorescein and Protein Assay Method for Fluorescent-Antibody Conjugates

1966 ◽  
Vol 14 (2) ◽  
pp. 271-275 ◽  
Author(s):  
Arthur F. Wells ◽  
Curtis E. Miller ◽  
Marvin K. Nadel
Keyword(s):  
Fluorescent Antibody ◽  
Assay Method ◽  
Protein Assay ◽  
Antibody Conjugates

scholarly journals Rapid Fluorescein and Protein Assay Method for Fluorescent-Antibody Conjugates

1966 ◽  
Vol 14 (2) ◽  
pp. 271-275
Author(s):  
Arthur F. Wells ◽  
Curtis E. Miller ◽  
Marvin K. Nadel
Keyword(s):  
Fluorescent Antibody ◽  
Assay Method ◽  
Protein Assay ◽  
Antibody Conjugates

scholarly journals Evaluation of Multivalent Leptospira Fluorescent Antibody Conjugates for General Diagnostic Use

1989 ◽  
Vol 1 (2) ◽  
pp. 146-149 ◽  
Author(s):  
David A. Miller ◽  
Mark A. Wilson ◽  
Clyde A. Kirkbride
Keyword(s):  
Fluorescent Antibody ◽  
Bacterial Species ◽  
Antibody Test ◽  
Additional Method ◽  
Diagnostic Laboratory ◽  
Bacterial Cultures ◽  
Typical Cell ◽  
Antibody Conjugates ◽  
Treponema Hyodysenteriae ◽  
Culture Positive

Four lots of conjugate were evaluated for optimal dilution and degree of fluorescence produced with reference cultures and bovine and porcine leptospira isolates. One lot that uniformly produced better fluorescence was evaluated for sensitivity and specificity with reference cultures, isolates, culture-positive tissues, and 13 other bacterial species. Further evaluation of the conjugates was done with bovine, porcine, and ovine specimens submitted to a diagnostic laboratory. Leptospires were detected with the fluorescent antibody test (FAT) in 9 of 21 culture-positive bovine kidneys and were detected in diluted cultures when present at concentrations of 102-1O3 organisms/ml. With the exception of Treponema hyodysenteriae, FAT's of other bacterial cultures produced minimal fluorescence or were negative. Positives were characterized by moderate to brilliant fluorescence of typical cell forms, and most nonspecific fluorescence was eliminated with a flazo-orange counter-stain. The results indicated that the FAT utilizing multivalent conjugates could be used successfully as an additional method for diagnosis of leptospira infections.

A sensitive protein assay method using micro-titer plates

1986 ◽  
Vol 42 (2) ◽  
pp. 161-162 ◽  
Author(s):  
K. Sorensen ◽  
U. Brodbeck
Keyword(s):  
Assay Method ◽  
Protein Assay

scholarly journals Preparation of Agglutinating Antisera and Fluorescent-Antibody Conjugates Against Pasteurella tularensis in Equines

1970 ◽  
Vol 19 (6) ◽  
pp. 894-897
Author(s):  
James H. Green ◽  
Richard C. Bolin ◽  
Russell K. Carver ◽  
Herman Gross ◽  
Nan Pigott ◽  
...  
Keyword(s):  
Fluorescent Antibody ◽  
Antibody Conjugates

scholarly journals Technical Considerations in the Preparation of Fluorescent-Antibody Conjugates

1964 ◽  
Vol 12 (4) ◽  
pp. 343-348 ◽  
Author(s):  
Vester J. Lewis ◽  
Wallis L. Jones ◽  
John B. Brooks ◽  
William B. Cherry
Keyword(s):  
Fluorescent Antibody ◽  
Antibody Conjugates ◽  
Technical Considerations

scholarly journals Technical Considerations in the Preparation of Fluorescent-Antibody Conjugates

1964 ◽  
Vol 12 (4) ◽  
pp. 343-348
Author(s):  
Vester J. Lewis ◽  
Wallis L. Jones ◽  
John B. Brooks ◽  
William B. Cherry
Keyword(s):  
Fluorescent Antibody ◽  
Antibody Conjugates ◽  
Technical Considerations
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