Production of Native-Type Streptoverticillium mobaraense Transglutaminase in Corynebacterium glutamicum
2003 ◽
Vol 69
(5)
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pp. 3011-3014
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ABSTRACT We previously observed secretion of active-form transglutaminase in Corynebacterium glutamicum by coexpressing the subtilisin-like protease SAM-P45 from Streptomyces albogriseolus to process the prodomain. However, the N-terminal amino acid sequence of the transglutaminase differed from that of the native Streptoverticillium mobaraense enzyme. In the present work we have used site-directed mutagenesis to generate an optimal SAM-P45 cleavage site in the C-terminal region of the prodomain. As a result, native-type transglutaminase was secreted.
1974 ◽
Vol 39
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pp. 1933-1939
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1980 ◽
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pp. 1144-1154
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1991 ◽
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pp. 7044-7050
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1976 ◽
Vol 251
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pp. 4365-4371
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1984 ◽
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pp. 14928-14934
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1982 ◽
Vol 257
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pp. 4522-4528
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1976 ◽
Vol 251
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pp. 5901-5903
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1980 ◽
Vol 44
(8)
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pp. 1945-1947
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1990 ◽
Vol 93
(4)
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pp. 378-384
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