scholarly journals Relationship between the limited and wide host range octopine-type Ti plasmids of Agrobacterium tumefaciens.

1981 ◽  
Vol 146 (2) ◽  
pp. 484-493 ◽  
Author(s):  
M F Thomashow ◽  
V C Knauf ◽  
E W Nester
Plasmid ◽  
1993 ◽  
Vol 29 (2) ◽  
pp. 154-159 ◽  
Author(s):  
Léon Otten ◽  
Jean-Claude Gérard ◽  
Patrice De Ruffray

1984 ◽  
Vol 3 (13) ◽  
pp. 3043-3047 ◽  
Author(s):  
A. Hoekema ◽  
B.S. de Pater ◽  
A.J. Fellinger ◽  
P.J.J. Hooykaas ◽  
R.A. Schilperoort

1998 ◽  
Vol 11 (5) ◽  
pp. 429-433 ◽  
Author(s):  
B. Schrammeijer ◽  
J. Hemelaar ◽  
P. J. J. Hooykaas

Octopine and nopaline strains of Agrobacterium tumefaciens differ in their ability to induce tumors on Nicotiana glauca. The presence of a virF locus on the octopine Ti plasmid makes N. glauca a host plant for these strains, indicating that the VirF protein is a host-range determinant. Here we show the presence of a virF locus not only on the Agrobacterium vitis octopine/cucumopine plasmids pTiAg57 and pTiTm4, but also on the nopaline Ti plas-mids pTiAT1, pTiAT66a, and pTiAT66b. On the octopine Ti plasmids from A. tumefaciens the virF gene is located between the virE locus and the left border of the T-region. In contrast, the virF gene on Ti plasmids of A. vitis is located at the very left end of the vir-region near the virA locus. The virF gene of pTiAg57 has been sequenced and codes for a protein of 202 amino acids with a molecular mass of 22,280 Da. Comparison showed that the virF gene from A. vitis strain Ag57 is almost identical to that from A. tumefaciens octopine strains. The transcription of the pTiAg57 virF is inducible by the plant phenolic compound acetosyringone through the presence of a vir-box consensus sequence in its promoter region. The VirF protein from pTiAg57 can complement octopine A. tumefaciens strains deleted for virF as shown by tumor formation on N. glauca.


1973 ◽  
Vol 12 (3) ◽  
pp. 734-741 ◽  
Author(s):  
Johng S. Rhim ◽  
Mina L. Vernon ◽  
Fuw G. Duh ◽  
Robert J. Huebner

2009 ◽  
Vol 99 (1) ◽  
pp. 58-66 ◽  
Author(s):  
Yosuke Matsushita ◽  
Kumar K. R. Penmetcha

Chrysanthemum stunt viroid (CSVd), a noncoding RNA, is known to cause chrysanthemum stunt disease, which affects the yield of flowers. To gain insights into CSVd replication, infection, and the reasons for the spreading of CSVd disease in chrysanthemum plants, we prepared linear CSVd RNA and analyzed its ability to cause disease in chrysanthemum plants. We found that linear CSVd replicated as efficiently as CSVd RNA isolated from the infected chrysanthemum plants. Additionally, the linear CSVd RNA was evaluated for its ability to infect other plants as well, which revealed that CSVd has a wide host range for its replication. Importantly, the CSVd isolated from these hosts is infectious to chrysanthemum plants, and thus potentially contributes to the spreading of the disease to chrysanthemum plants.


1990 ◽  
Vol 92 (4) ◽  
pp. 1226-1232 ◽  
Author(s):  
Anne-Marie Stomp ◽  
Carol Loopstra ◽  
W. Scott Chilton ◽  
Ronald R. Sederoff ◽  
Larry W. Moore

Sign in / Sign up

Export Citation Format

Share Document